Role of the plasmid-encoded tet(O) gene in tetracycline-resistant clinical isolates of Campylobacter jejuni and Campylobacter coli

Abstract

The prevalence of tetracycline resistance, tetracycline MICs and tet(O) gene localization were investigated in 83 Campylobacter isolates from patients suffering from acute gastroenteritis in Germany. Combined biochemical and molecular markers identified 74 isolates (89 %) as Campylobacter jejuni, including seven atypical isolates that failed to hydrolyse hippurate, and nine isolates (11 %) as Campylobacter coli. Tetracycline resistance was detected in six out of nine Campylobacter coli isolates (67 %) and 13 out of 74 C. jejuni isolates (18 %). Low-level tetracycline resistance was observed for C. coli (MIC 16 microg ml(-1) for all strains), whereas C. jejuni showed high-level resistance (MIC >256 microg ml(-1) for all strains). Both low- and high-level tetracycline resistance was associated with the presence of the tet(O) gene. In C. jejuni, tet(O) was plasmid-encoded in 54 % of tetracycline-resistant isolates, whereas in C. coli, tet(O) appeared to be located on the chromosome.

Campylobacter jejuni proteins Cj0952c and Cj0951c affect chemotactic behaviour towards formic acid and are important for invasion of host cells

“…The C. jejuni strains used in this study were B2, which was isolated in the University Medical Center Göttingen from a patient suffering from gastroenteritis (Schmidt-Ott et al, 2005;Dasti et al, 2007), and the C. jejuni strains 81-176 and NCTC 11168. Bacteria were routinely grown on Columbia blood agar supplemented with 5 % defibrinated sheep blood under microaerophilic conditions (85 % N 2 , 10 % CO 2 , 5 % O 2 ) at 42 uC for 24 h. When needed, appropriate antibiotics were supplemented at the following concentrations: kanamycin (50 mg ml 21 ) or chloramphenicol (30 mg ml 21 ).…”

“…Random insertion of the transposons was verified for a fraction of 19 mutants. After preparation of genomic DNA and digestion with BspHI, a Southern blot analysis was carried out with a digoxigenin-11-UTP-probed kanamycin-resistance gene under conditions described previously (Dasti et al, 2007).…”

Sulphite : cytochrome c oxidoreductase deficiency in Campylobacter jejuni reduces motility, host cell adherence and invasion

“…C. jejuni strain B2, isolated from a patient suffering from gastroenteritis, was grown on Columbia agar supplemented with 5 % defibrinated sheep blood at 42 uC in microaerobic conditions (85 % N 2 , 10 % CO 2 , 5 % O 2 ) (Schmidt-Ott et al, 2005;Dasti et al, 2007). When needed, kanamycin and chloramphenicol were added at concentrations of 50 mg ml 21 and 30 mg ml 21 , respectively.…”

Livestock Production and Foodborne Diseases from Food Animals in Thailand

“…S in c e mo s t fluoroquinolone resistance in C. Jejuni in Thailand was mediated by mutation in the gyrA gene [32], which can not transfer horizontally, it is likely that Campylobacter develops resistance in food animals during the raising period on farms, and subsequently infects humans. However, for other antimicrobial agents with mobile resistance genes, Campylobacter may develop resistance in food animals and transfer the resistance gene to other human pathogens similar to Salmonella [4,12].…”