Role of the Pentose Phosphate Pathway in Pasteurella multocida

Issaly, Inda M.; Issaly, A. S.; Stoppani, A. O. M.

doi:10.1038/191727b0

Cited by 3 publications

(3 citation statements)

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“…The upregulation of genes in the pentose phosphate pathways suggested that P. multocida had to adjust its metabolic pathways during the growth in conditioned media to earn necessary nutrients (sugars and amino acids) for survival. This pentose phosphate pathway is the main path of carbohydrate oxidation in P. multocida, involving the growth and resting stages of the bacterial cells [44]. The dhaKL genes were part of the phosphotransferase system (PTS), which controlled carbohydrate uptake and carbon metabolism.…”

Section: Discussionmentioning

confidence: 99%

Effect of conditioned media from Aeromonas caviae on the transcriptomic changes of the porcine isolates of Pasteurella multocida

2022

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Background Pasteurella multocida is an opportunistic pathogen causing porcine respiratory diseases by co-infections with other bacterial and viral pathogens. Various bacterial genera isolated from porcine respiratory tracts were shown to inhibit the growth of the porcine isolates of P. multocida. However, molecular mechanisms during the interaction between P. multocida and these commensal bacteria had not been examined. Methods This study aimed to investigate the interaction between two porcine isolates of P. multocida (PM2 for type D and PM7 for type A) with Aeromonas caviae selected from the previously published work by co-culturing P. multocida in the conditioned media prepared from A. caviae growth and examining transcriptomic changes using RNA sequencing and bioinformatics analysis. Results In total, 629 differentially expressed genes were observed in the isolate with capsular type D, while 110 genes were significantly shown in type A. High expression of genes required for energy metabolisms, nutrient uptakes, and quorum sensing were keys to the growth and adaptation to the conditioned media, together with the decreased expression of those in the unurgent pathways, including translation and antibacterial resistance. Conclusion This transcriptomic analysis also displayed the distinct capability of the two isolates of P. multocida and the preference of the capsular type A isolate in response to the tough environment of the A. caviae conditioned media. Therefore, controlling the environmental sensing and nutrient acquisition mechanisms of P. multocida would possibly prevent the overpopulation of these bacteria and reduce the chance of becoming opportunistic pathogens.

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Section: Discussionmentioning

confidence: 99%

Effect of conditioned media from Aeromonas caviae on the transcriptomic changes of the porcine isolates of Pasteurella multocida

2022

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“…Materials and methods, unless stated otherwise were as described previously ( 1 ) . Preparation of disrupted-cell suspensions.…”

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“…The serine group (glycine, serine, cystine and cysteic acid) fixed 17-13% of total C14 in this fraction. multocida for synthesis of amino acids (Table VI) and purine bases ( 13). Alanine was labeled also by formate-C14 in a significant proportion especially in the presence of glucose.…”

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confidence: 99%