Role of the Nitrogen‐fixing Bacterial Microflora in the Epiphytism of Tillandsia (Bromeliaceae)

Brighigna, Luigi; Montaini, P.; Favilli, Franco; Trejo, Alfonso

doi:10.1002/j.1537-2197.1992.tb13646.x

Cited by 42 publications

(29 citation statements)

References 24 publications

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“…Nitrogen is a limited factor for tank bromeliads. The plants receive nitrogen by mineralization of organic material from the canopy, from atmospheric sources (Stewart et al 1995), by interactions with animals (Davidson and Epstein 1989;Leroy et al 2013) or probably by microbial N 2 fixation (Brighigna et al 1992).…”

Section: Discussionmentioning

confidence: 99%

Bromeliad tanks are unique habitats for microbial communities involved in methane turnover

2016

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Background and aims Tank bromeliads collect organic matter and rainwater (= tank slurry) between their densely arranged leaf axils for their nutrient demand. Diverse communities of microorganisms inhabit these tanks and are responsible for the breakdown of organic matter. Anaerobic degradation results in the release of substantial amounts of methane. We hypothesized that each individual bromeliad harbors its own microbial community, which is affected by chemical tank-slurry properties. We further hypothesized that methanotrophic bacteria inhabit bromeliad tank slurries, potentially able to oxidize the produced CH 4 . Methods We investigated communities of Bacteria, Archaea, methanogenic and methanotrophic microorganisms measuring their abundance (qPCR) and composition (TRFLP) within eight bromeliad tanks of the species Werauhia gladioliflora sampled in a Costa Rican lowland forest. Tank slurries were analyzed for pH, carbon, nitrogen, oxygen and fatty acid concentrations. Methane oxidation rates were determined in five bromeliad tank slurries.Results Our results showed that microbial communities differed between plants and were affected by chemical tank slurry properties. Further, not only methanogenic archaea but also methanotrophic bacteria were detected in the tanks of all bromeliad plants, the latter being potentially able to aerobically oxidize between 25 and 62 μg CH 4 gdw. Conclusion Our results indicate that every bromeliad tank is a unique island with respect to its resident microbial community. The presence of methanogens and active methanotrophs in all tank slurries further indicates the potential for both methane formation and methane oxidation.

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Section: Discussionmentioning

confidence: 99%

Bromeliad tanks are unique habitats for microbial communities involved in methane turnover

2016

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“…In procedure C, semisolid NFCC medium prepared in culture tubes was inoculated with previously used soil dilutions and incubated under 21% O 2 for 7 days, followed by two other transfers of 1 ml of inoculum to fresh medium. After the third incubation, plating was carried out on solid NFCC medium containing 50 mM NH 4 Cl. Plates were incubated under atmospheric conditions for 21 days.…”

Section: Methodsmentioning

confidence: 99%

“…(A) Strategy A, direct plating on solid N-free medium incubated inside a hypoxic chamber (2% O 2 ); (B) strategy B, inoculation into semisolid N-free medium with incubation in air for a week, followed by serial dilution and plating on solid N-free medium and incubation inside a hypoxic chamber 2% O 2 ; (C) strategy C, inoculation into semisolid N 2 -free medium with incubation in air with three subsequent transfers in the same semisolid medium before plating on solid medium amended with NH 4 Cl. The boxed area represents the 2% O 2 condition in the hypoxic chamber.…”

Section: Fig 1 Different Strategies Used For the Isolation Of Free-limentioning

confidence: 99%

Development of a Direct Isolation Procedure for Free-Living Diazotrophs under Controlled Hypoxic Conditions

Mirza

Rodrigues

2012

Appl Environ Microbiol

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Free-living diazotrophs are diverse and ubiquitous in soil, contributing the nitrogen pool in natural ecosystems. The isolation of nitrogen-fixing microorganisms has relied on semisolid nitrogen-free medium enrichment, followed by multiple subculturing steps. These procedures limit the diversity of recovered isolates. In the current study, we investigated three different isolation strategies for free-living diazotrophs using a soil sample from the Amazon forest. The methods were (i) direct plating on solid nitrogen-free medium under a 2% O 2 concentration, (ii) enrichment in semisolid nitrogen-free medium before plating on solid nitrogen-free medium under 2% O 2 , and (iii) enrichment followed by subculturing in the semisolid nitrogen-free medium before plating on nitrogen containing medium under a 21% O 2 concentration. A total of 794 isolates were differentiated by their genomic fingerprinting patterns, and strains with unique profiles were identified on the basis of sequencing of their 16S rRNA gene. Isolates belonged to four bacterial phyla: Proteobacteria, Firmicutes, Actinobacteria, and Bacteriodetes. The novel strategy of combining a solid N-free medium and hypoxic conditions showed an increase of 62.6% in the diversity of diazotrophs in comparison to that obtained by the conventional semisolid medium-based methods. All isolates grew on the nitrogen-free medium under a 2% O 2 concentration, 78% of them showed the presence of the nifH gene, and 39% tested positive for acetylene reduction activity. Our results suggest that direct plating of soil dilutions on nitrogen-free solid medium under a 2% O 2 concentration is a useful strategy for the isolation of the diverse diazotrophic communities.

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“…aquatilis (YL-1 and ML-1), Pantoea agglomerans (SL-1), Klebsiella terrigena (tentative, SL-2), and Erwinia rhapontici (SL-3), respectively, all of which had been reported as phylloplane W phyllosphere or rhizoplane bacteria. [6][7][8][9] In a comparison of phenotypic and physiological characteristics with those of the standard species in Bergey's Manual of Determinative Bacteriology, 9th Edition, 10)ˆv e isolates had certain divergences in their cell size, oxidase activity, catalase activity, H2S production, and so on (See footnotes in Table 1). However, these characteristics showed clear convergence amongst the isolates of four diŠerent species inhabiting the Ro.…”

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confidence: 99%

Characterization of Five Phyllosphere Bacteria Isolated fromRosa rugosaLeaves, and Their Phenotypic and Metabolic Properties

Hashidoko

Itoh

Yokota

et al. 2002

Bioscience, Biotechnology, and Biochemistry

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Role of the Nitrogen‐fixing Bacterial Microflora in the Epiphytism of Tillandsia (Bromeliaceae)

Cited by 42 publications

References 24 publications

Bromeliad tanks are unique habitats for microbial communities involved in methane turnover

Bromeliad tanks are unique habitats for microbial communities involved in methane turnover

Development of a Direct Isolation Procedure for Free-Living Diazotrophs under Controlled Hypoxic Conditions

Characterization of Five Phyllosphere Bacteria Isolated fromRosa rugosaLeaves, and Their Phenotypic and Metabolic Properties

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