Role of the net electrical charge of the complete antigen in determining the chemical nature of anti-p-azobenzenearsonate antibodies

Rüde, Erwin; Mozes, E; Sela, Michael

doi:10.1021/bi00848a038

Cited by 20 publications

(1 citation statement)

References 26 publications

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“…Much work has been done in an attempt to elucidate the mechanism of antibody formation. Rude et al (1968), for example, have reported the correlation between net electrical charge on the antigen to that on the antibody it produces. Based on these observations, antibodies to acidic and basic antigens could be separated by ion exchange chromatography.…”

Section: Discussionmentioning

confidence: 99%

Effect of heat on peanut proteins. I. Solubility properties and immunochemical-electrophoretic modifications

Neucere¹

1972

J. Agric. Food Chem.

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Section: Discussionmentioning

confidence: 99%

Effect of heat on peanut proteins. I. Solubility properties and immunochemical-electrophoretic modifications

Neucere¹

1972

J. Agric. Food Chem.

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Antibodies against polyalanyl determinants attached to negatively or positively charged carriers

1971

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This specificity is also shown b y absorption data, results that, in addition, confirmed the basic concept of anti-RS antiserum formation. That lymphoid cells from animals that were not expected to possess a particular RS, such as those from, for instance, (A x C57BL/6) F1 hybrids or from A mice in the case of RS(A), failed to neutralize serum anti-RS(A), strongly favors the view that animals lacking a particular RS may be provoked to produce an antiserum to that RS. As far as neutralization of anti-RS activity by absorption with lymphoid cells is concerned, failure t o neutralize extended even t o lymphoid cells from specifically tolerant animals. This result merely reflects classical ideas on transplantation tolerance implying central failure of t h e immune system t o the tolerated antigens [ 11. Of course, neutralization of a particular murine anti-RS serum should be possible b y absorption with any mouse lymphoid cells carrying corresponding RS. Results t o b e published will demonstrate the validity of this fact. A complication of these experiments stems from the fact that parental strain lymphoid cells used t o immunize F 1 hybrids are, for genetic reasons, accepted by the hosts and are thus able to form classical alloantibodies against the transplantation antigens of the other parental strain present in the hybrids. Such antibodies could have an "enhancementlike" effect o n the recognition process by masking of the antigenic target. Appropriate experiments have shown that this is indeed the case. The effect can be easily distinguished from the anti-RS activity discussed here: it addresses itself t o the target cell of immunological one-way reactions and obeys opposite laws of absorption. The Weizmann Institute of Science, Rehovot Antibodies against polyalanyl determinants attached to negatively or positively charged carriers Polyalanyl determinants, when attached to acidic or basic carriers, provoke a specific anti-polyalanyl antibody population, the majority of which possesses an electrical charge which is inversely related t o that of the carrier. The .alanine chains in the immunogens form complete determinants and the contribution of the carrier t o the specificity of the antibodies is minimal. The antipolyalanyl antibodies, when fractionated into a more acidic and a more basic fraction, possess similar properties of their combining sites, as judged from hapten inhibition and equilibrium dialysis studies. Thus, the specificity of the antibodies and the combining site features are determined by the hapten determinants, whereas the net charge of the antibodies is determined by the net charge of the immunogen. The difference in the net electrical charge of the antibodies may reflect differences in areas of the molecule other than the combining site. A comparison of anti-polyalanyl antibodies obtained with different carriers has shown that the specificity and size of the combining sites are similar regardless of the nature of the carrier molecule, whereas different affinities of the antibodies are obtained...

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The influence of the net charge of antigen on the distribution of bovine IgG class antibodies

et al. 1973

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It has previously been shown that bovine immunoglobulins of the more positively charged IgGz class are confined exclusively to the serum and are incapable Max-Planck-lnstitut fur I mmunbiologie, Freiburg/Brsg. of being transferred t o the colostrum by t h e dry mammary gland. Since recent studies have demonstrated that antigens with a negative net charge preferentially provoke the synthesis of more positively charged antibodies, it could be expected that antibodies against such antigens would not be transferred t o the colostrum. In the present study, however, acidic antigens (p-azobenzenearsonate edestin and its maleylated derivative) failed t o stimulate predominantly the synthesis of precipitating, binding and phage neutralizing antibodies of the positively charged IgC, class. As expected, immunization of cattle with iysozyme, (a basic antigen), resulted in the production of antibodies almost exclusively confined to the more negatively charged IgG, fraction. In analyzing the precipitin reaction of IgG, class antibodies, it could be shown that maximum precipitation was achieved at pH 5.0 and decreased significantly at higher pH values.

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Role of the net electrical charge of the complete antigen in determining the chemical nature of anti-p-azobenzenearsonate antibodies

Cited by 20 publications

References 26 publications

Effect of heat on peanut proteins. I. Solubility properties and immunochemical-electrophoretic modifications

Effect of heat on peanut proteins. I. Solubility properties and immunochemical-electrophoretic modifications

Antibodies against polyalanyl determinants attached to negatively or positively charged carriers

The influence of the net charge of antigen on the distribution of bovine IgG class antibodies

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