Role of the N-linked glycans of the prM and E envelope proteins in tick-borne encephalitis virus particle secretion

Goto, Akiko; Yoshii, Kentarou; Obara, Mayumi; Ueki, Tomotaka; Mizutani, Tetsuya; Kariwa, Hiroaki; Takashima, Ikuo

doi:10.1016/j.vaccine.2004.11.068

Cited by 61 publications

(62 citation statements)

References 34 publications

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“…We previously examined the role of the N-linked glycans of E protein in tick-borne encephalitis-virus particle secretion using subviral particles. 13 Secretion of virus particles was greatly reduced in culture cells transfected with mutant vectors that have an amino acid substitution of T156A in the E protein, and the study also suggested that the reduced particle secretion is caused by glycan loss rather than to the amino acid substitution per se. The amino acid substitution of T156A is similar to that of S156P in our current study in terms of amino acid characteristics, and both mutations altered the protein such that it would not be recognized by oligosaccharyl-transferase.…”

Section: Discussionmentioning

confidence: 93%

“…23 -25 But there was no report studying the relationship among temperature sensitivity of WN virus, the glycosylation of envelope protein, and the intracellular viral protein maturation and trafficking. Our previous study 13 using a subviral system of tick-borne encephalitis virus showed that a mutant lacking E-protein glycosylation has a large reduction in the level of secretion of the E protein; the E protein is retained at the endoplasmic reticulum and is rarely present in the Golgi complex. In the dengue virus, this glycosylation at aa154 occurs in E-protein domain I, close to the center of the fusion peptide of E-protein domain II, and glycosylation of the E protein is considered to increase the stability of the protein.…”

Section: Discussionmentioning

confidence: 99%

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Glycosylation of the West Nile Virus Envelope Protein Increases In Vivo and In Vitro Viral Multiplication in Birds

Murata¹,

Eshita²,

Maeda³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. Many West Nile (WN) virus isolates associated with significant outbreaks possess a glycosylation site on the envelope (E) protein. E-protein glycosylated variants of New York (NY) strains of WN virus are more neuroinvasive in mice than the non-glycosylated variants. To determine how E protein glycosylation affects the interactions between WN virus and avian hosts, we inoculated young chicks with NY strains of WN virus containing either glycosylated or nonglycosylated variants of the E protein. The glycosylated variants were more virulent and had higher viremic levels than the non-glycosylated variants. The glycosylation status of the variant did not affect viral multiplication and dissemination in mosquitoes in vivo . Glycosylated variants showed more heat-stable propagation than non-glycosylated variants in mammalian (BHK) and avian (QT6) cells but not in mosquito (C6/36) cells. Thus, E-protein glycosylation may be a requirement for efficient transmission of WN virus from avian hosts to mosquito vectors.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Glycosylation of the West Nile Virus Envelope Protein Increases In Vivo and In Vitro Viral Multiplication in Birds

Murata¹,

Eshita²,

Maeda³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…Although glycosylation of flavivirus premembrane proteins appears to be absolutely required for efficient virion assembly and maturation, 16,[25][26][27][28] WNV strains lacking a glycosylation site in the E protein have been isolated from naturally infected vertebrates and mosquitoes. [8][9][10][11][12][13] Previously, we have shown that WNV lacking the E protein glycan is attenuated in the natural vector species Cx.…”

Section: Discussionmentioning

confidence: 99%

Requirement of Glycosylation of West Nile Virus Envelope Protein for Infection of, but Not Spread within, Culex quinquefasciatus Mosquito Vectors

Moudy¹,

Payne²,

Dodson³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Most of sequenced West Nile virus (WNV) genomes encode a single N-linked glycosylation site on their envelope (E) proteins. We previously found that WNV lacking the E protein glycan was severely inhibited in its ability to replicate and spread within two important mosquito vector species, Culex pipiens and Cx. tarsalis. However, recent work with a closely related species, Cx. pipiens pallens, found no association between E protein glycosylation and either replication or dissemination. To examine this finding further, we expanded upon our previous studies to include an additional Culex species, Cx. quinquefasciatus. The non-glycosylated WNV-N154I virus replicated less efficiently in mosquito tissues after intrathoracic inoculation, but there was little difference in replication efficiency in the midgut after peroral infection. Interestingly, although infectivity was inhibited when WNV lacked the E protein glycan, there was little difference in viral spread throughout the mosquito. These data indicate that E protein glycosylation affects WNV–vector interactions in a species-specific manner.

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Section: Discussionmentioning

confidence: 99%

“…In one study, the correlation between the presence of such sites and antigenic properties of flavivirus was not established because deglycosylated viruses could maintain the same antigenicity (Winkler et al, 1987). On the other hand, in other more recent studies, glycosylation was found to be very important for viral replication, virulence, maturation or release of viral-like particles (Beasley et al, 2005;Crabtree et al, 2005;Goto et al, 2005;Li et al, 2006).Our analysis of the conserved motifs showed that ROCV shares numerous conserved amino acid and/or peptides with several flaviviruses across different groups, each with a varied host range. Interestingly, in the E protein, the tripeptide (TGP) of ROCV within domain III was found to be different from those observed in all other JEV members (RGX, where X is D, E or T).…”

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confidence: 99%

Complete genome characterization of Rocio virus (Flavivirus: Flaviviridae), a Brazilian flavivirus isolated from a fatal case of encephalitis during an epidemic in São Paulo state

Medeiros

Nunes

Vasconcelos

et al. 2007

Journal of General Virology

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The flaviviruses of major medical importance in South American countries are yellow fever, dengue, Saint Louis encephalitis, West Nile and Rocio viruses. Rocio virus (ROCV) has been responsible for epidemics of severe encephalitis in Brazil with a case-fatality rate of 10 % and development of sequelae in 20 % of the survivors. We have sequenced and characterized the entire genome of ROCV for the first time, by determining the general traits of the open reading frame and the characteristics of viral genes including the potential cleavage sites, conserved or unique motifs, cysteine residues and potential glycosylation sites. The conserved sequences in the 39-non-coding region were identified, and the predicted secondary structures during cyclization between 59-and 39-non-coding regions were studied. Multiple protein and phylogenetic analyses based on antigenically important and phylogenetically informative genes confirmed a close relationship between ROCV and Ilheus virus (ILHV), together constituting a unique and distinct phylogenetic subgroup as well as the genetic relationship of ROCV with several members of the Japanese encephalitis group. Although ROCV is phylogenetically related to ILHV, our study shows that it is still a virus distinct from the latter virus. This is the first flavivirus uniquely indigenous to Brazil that has been sequenced completely and the genome characterized. The data should be useful for further studies at the molecular level, including construction of infectious clone, identification of gene function, improved disease surveillance based on molecular diagnostic tools and vaccine development. INTRODUCTIONIn South America, until the mid-1970s, the two neurotropic flaviviruses had been Saint Louis encephalitis virus (SLEV) and Ilheus virus (ILHV). However, in contrast to North America, an epidemic of encephalitis in humans by SLEV in South America was not recorded for many years until the first outbreak in Argentina in 2005(Diaz et al., 2006, despite accumulated records of virus isolation from mosquitoes and vertebrate hosts throughout the continent (Spence, 1980). As for the other virus, ILHV, human infection was sporadic and development of neurotropic manifestations has been even more infrequent. Thus, the sudden emergence of an outbreak of encephalitis caused by a flavivirus in 1974-75 in Brazil was unexpected.Rocio virus (ROCV) belongs to the genus Flavivirus in the family Flaviviridae. Its prototype (strain SPH 34675) was isolated in Ribeira Valley in the south-eastern São Paulo state of Brazil in 1975 from the cerebellum of a fatal human case during an epidemic of encephalitis that could not be readily diagnosed with the reagents then available for known encephalitic viruses. The epidemic, which began in 1974, spread to more than 20 municipalities over the following 2 years causing approximately 1000 diagnosed cases.Members of the genus Flavivirus (hereafter called flaviviruses) are positive-sense, single-stranded RNA viruses that infect humans and other vertebrates. They are mainly...

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Role of the N-linked glycans of the prM and E envelope proteins in tick-borne encephalitis virus particle secretion

Cited by 61 publications

References 34 publications

Glycosylation of the West Nile Virus Envelope Protein Increases In Vivo and In Vitro Viral Multiplication in Birds

Glycosylation of the West Nile Virus Envelope Protein Increases In Vivo and In Vitro Viral Multiplication in Birds

Requirement of Glycosylation of West Nile Virus Envelope Protein for Infection of, but Not Spread within, Culex quinquefasciatus Mosquito Vectors

Complete genome characterization of Rocio virus (Flavivirus: Flaviviridae), a Brazilian flavivirus isolated from a fatal case of encephalitis during an epidemic in São Paulo state

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