Role of the midgut gland in metabolism and excretion of ecdysteroids by lobsters, Homarus americanus

Snyder, Mark J.; Chang, Ernest S.

doi:10.1016/0016-6480(92)90013-a

Cited by 17 publications

(13 citation statements)

References 36 publications

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“…These conjugates can be formed from ecdysteroids in the hemolymph [63,65,66,84,97] or from ecdysteroids ingested with food [97]. When lobsters were fed [ 3 H]ecdysone, less than 0.4% of the radioactivity appeared in the hemolymph and urine; essentially all of the [ 3 H]ecdysone was in the form of apolar conjugates in the feces [97].…”

Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

“…Various tissues can convert ecdysteroids and their metabolites to highly polar (HP) conjugates, which can be excreted in the urine [61,63,65,66,97]. The levels of HP conjugates vary over the molt cycle and can at times be the predominant ecdysteroid in the hemolymph [61,77,82,87,88,93].…”

Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

“…Oxidation at the C26 forms ecdysonoic acids (EAs). For example, 20E and PA are metabolized to 20-hydroxyecdysonoic acid (20EA) and 25-deoxy-20-hydroxyecdysonoic acid (25d20EA), respectively, and are excreted in the urine [65][66][67]97,102]. Ecdysteroids can also be converted to their 3␣-hydroxy epimers by peripheral tissues.…”

Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

“…The hepatopancreas is the primary site for the production of apolar conjugates (perhaps fatty acyl esters), which are then eliminated in the feces [65,66,99]. These conjugates can be formed from ecdysteroids in the hemolymph [63,65,66,84,97] or from ecdysteroids ingested with food [97].…”

Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

“…In insects, 20E is inactivated by a cytochrome P450 26-hydroxylase [100,101]. In decapod crustaceans, a 26-hydroxylase activity in various tissues converts 20E to 20,26-dihydroxyecdysone (20,26E) and PA to inokosterone (25-deoxy-20,26-dihydroxyecdysone or 25d20,26E) [63,[65][66][67]97]. Oxidation at the C26 forms ecdysonoic acids (EAs).…”

Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

See 4 more Smart Citations

Ecdysteroid metabolism in crustaceans

Mykles

2011

The Journal of Steroid Biochemistry and Molecular Biology

173

121

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Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

Section: Ecdysteroid Metabolism and Excretionmentioning

confidence: 99%

See 3 more Smart Citations

Ecdysteroid metabolism in crustaceans

Mykles

2011

The Journal of Steroid Biochemistry and Molecular Biology

173

121

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Identification of a New Cytochrome P450 Family, CYP45, from the Lobster,Homarus americanus,and Expression Following Hormone and Xenobiotic Exposures

Snyder

1998

Archives of Biochemistry and Biophysics

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An eclosion hormone-like gene participates in the molting process of Palaemonid shrimp Exopalaemon carinicauda

Zhou

Wang

et al. 2017

Dev Genes Evol

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Molting behavior is an important physiological process related to metamorphosis, growth, and reproduction in crustaceans. Previous studies indicated that the molting process was controlled by 20-hydroxyecdysone (20E) and upstream hormones, peptides, and environmental factors, which regulate 20E function. Eclosion hormone (EH) in insect is a kind of neuropeptide that is regulated by 20E and triggers ecdysis behavior at the end of molting process. However, the function of eclosion hormone gene during the molting process in crustaceans is still largely unknown. In the present study, an eclosion hormone-like gene EcEHL was identified from Exopalaemon carinicauda. The deduced amino acid sequence of EcEHL contained a signal peptide, a typical eclosion domain, and six conserved cysteine residues forming three putative disulfide bonds. EcEHL was predominantly expressed in the epidermis, gill, and eyestalk of shrimp. In situ hybridization analysis showed that EcEHL transcripts were localized in gill cells and in medulla externa X-organ, medulla terminalis X-organ, sinus gland, and lamina ganglionaris of eyestalks. During the molting process of shrimp, EcEHL showed the highest expression level in shrimp at the premolt stage. The expression level of EcEHL in shrimp at mid premolt stage was up-regulated by injection of exogenous 20E. Silencing of EcEHL using double-stranded RNA delayed both the molting process and ecdysis rate of E. carinicauda. Furthermore, injection of exogenous 20E to shrimp at mid premolt stage (D2) could remarkably speed up the molting process and also raise the ecdysis rate of E. carinicauda. The results revealed that EcEHL might participate in the molting process of shrimp and its expression was regulated by 20E. These data will help us to understand the molecular mechanism of molting in crustacean.

show abstract

Role of the midgut gland in metabolism and excretion of ecdysteroids by lobsters, Homarus americanus

Cited by 17 publications

References 36 publications

Ecdysteroid metabolism in crustaceans

Ecdysteroid metabolism in crustaceans

Identification of a New Cytochrome P450 Family, CYP45, from the Lobster,Homarus americanus,and Expression Following Hormone and Xenobiotic Exposures

An eclosion hormone-like gene participates in the molting process of Palaemonid shrimp Exopalaemon carinicauda

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