Role of the MHC2TA gene in autoimmune diseases

Specific association of a CLEC16A/KIAA0350 polymorphism with NOD2/CARD15− Crohn's disease patients

“…20 Various microbial pathogens use the host's inhibitory SOCS proteins for manipulating cytokine receptor signaling, a helpful strategy to evade otherwise detrimental immune responses. Interestingly, located upstream of the CLEC16A gene is the human class II transactivator MHC2TA gene, and a haplotype conformed by two polymorphisms in the latter (rs3087456 and rs4774) was recently found associated with rheumatoid arthritis and MS. 21 Although no overall association with IBD was detected, stratification of CD patients by NOD2/CARD15 polymorphisms could evidence an effect. The obvious question that arises is why this locus was overlooked in the recent largest GWA study for CD.…”

Influence of Periostin on Synoviocytes in Knee Osteoarthritis

“…The PCR assays were performed as follows: denaturation for 10 min at 95˚C, 40 cycles of denaturation at 95˚C for 15 s, and annealing and extension at 60˚C for 1 min. Samples were analysed using an ABI Prism 7900HT Fast Real-Time PCR System (Applied Biosystems) (21,22). Relative quantification (23) was performed using the collected data (threshold cycle numbers, referred to as Ct) with an ABI Prism 7900HT Sequence-Detection System (SDS) software 2.3 (Applied Biosystems).…”

Influence of Mechanical Force on Bone Matrix Proteins in Ovariectomised Mice and Osteoblast-like MC3T3-E1 Cells

“…PCR assays were conducted as follows: 10 min denaturation at 95˚C, 40 cycles of 15 s, denaturation at 95˚C, and 1 min annealing and extension at 60˚C. Samples were analysed using an ABI Prism 7900HT Fast RT-PCR System (Applied Biosystems) (28,29). Relative quantification Statistical analysis.…”