Role of the interaction between puerarin and the erythrocyte membrane in puerarin-induced hemolysis

Hou, Shao Zhen; Su, Zi-Ren; Chen, Shu Xian; Ye, Mu Rong; Huang, Song; Liu, Liang; Zhou, Hua; Lai, Xiao Ping

doi:10.1016/j.cbi.2011.03.007

Cited by 35 publications

(20 citation statements)

References 37 publications

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“…11 In experimental models it is also suggested to be used in the prevention and treatment of cardiovascular diseases, diabetes, cancer and osteoporosis. 12 Ursolic acid (3β-hydroxy-urs-12-en-28-oic acid) is a pentacyclic triterpenoid obtained from plants. It has long been used in traditional Chinese medicine because of its anti-inflammatory, anti-arthritic, cytostatic and anti-proliferative, hepatoprotective effects.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Cytotoxicity Profiles of Different Phytochemicals: Comparison of Neutral Red and MTT Assays in Different Cells in Different Time Periods

Bacanlı

Anlara

Başaran

et al. 2017

tjps

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ÖZAmaç: Fenolik bileşikler sağlığı koruyucu farklı özellikler gösterir. Galangin, kurkumin, piknogenol, puerarin ve ursolik asit halk tıbbında yaygın olarak kullanılan bitkisel fenoliklerdendir. Bu çalışmanın amacı, nötral kırmızı alım (NKA) ve MTT yöntemleri arasındaki farkı sağlıklı hücreler ve kanser hücrelerinde farklı bitkisel fenoliklerin (galangin, kurkumin, piknogenol, puerarin ve ursolik asit) farklı zaman aralıklarında farklı zaman aralıklarında belirlemektir. Gereç ve Yöntemler: Bu çalışmada, bu fenolik bileşiklerin sitotoksik etkileri sağlıklı hücreler (Çin hamster fibroblast hücre hattı, V79) ve kanser [insan serviks epitelyal adenokarsinoma hücre hattı, Henrietta Lacks (HeLa) ve insan meme karsinoma hücre hattı (BT-474)] hücrelerinde 18, 24 ve 48 saatlik inkübasyon sürelerinde NKA ve MTT yöntemleriyle değerlendirilmiştir. Bulgular: Bulgularımız galangin, kurkumin, piknogenol, puerarin ve ursolik asitin V79, HeLa ve BT-474 hücre canlılıklarını 18, 24 ve 48 saatlik inkübasyon sürelerinde doza bağımlı olarak azalttığını göstermiştir. Ancak en az hücre canlılık oranı 48 saatlik inkübasyon sonrası görülmüştür. NKA ile MTT yöntemlerinin sonuçları arasında fark görülmemiştir. Sonuç: Sitotoksisite analizinde kullanılacak yöntem ve inkübasyon süresinin belirlenmesi için maddelerin sitotoksisite mekanizması bilinmelidir. Anahtar kelimeler: MTT, nötral kırmızı, bitkisel fenoliklerObjectives: Phenolic compounds exhibit several health protective properties. Galangin, curcumin, pycnogenol, puerarin and ursolic acid are commonly used plant phenolics in folk medicine. The aim of our study was to evaluate the difference between neutral red uptake (NRU) and MTT assays using different plant phenolics (galangin, curcumin, pycnogenol, puerarin and ursolic acid) in healthy and cancer cells in different time periods. Materials and Methods: In this study, the cytotoxic effects of these phenolic compounds were investigated by NRU and MTT assays in healthy (V79, Chinese hamster fibroblast cell line) and cancer [human cervix epithelial adenocarcinoma cell line Henrietta Lacks (HeLa) and human mammary carcinoma cell line (BT-474)] in 18, 24 and 48 h incubation periods. Results: Our results demonstrated that galangin, curcumin, pycnogenol, puerarin and ursolic acid decreased cell viability of V79, HeLa and BT-474 cells in a dose-dependent manner in 18, 24 and 48 h incubation periods. However, the cell survival rate was much lower in 48 h incubation period. There was no difference between the results from NRU and MTT assays. Conclusion: To decide which incubation period and which cytotoxicity study to be used, the cytotoxicity mechanism of the compound must be known.

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Section: Introductionmentioning

confidence: 99%

Assessment of Cytotoxicity Profiles of Different Phytochemicals: Comparison of Neutral Red and MTT Assays in Different Cells in Different Time Periods

Bacanlı

Anlara

Başaran

et al. 2017

tjps

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“…46 Membrane fluidity is an important parameter relating to the structure and functional state of the cell membrane. 21 The fluorescent probes, DPH and TMA-DPH, were incorporated into the rat erythrocyte membranes to detect the changes in membrane fluidity in the region near the center of the bilayer and in the outer leaflet of the membrane, respectively.…”

Section: Effect Of Surfactant Treatment On Membrane Fluiditymentioning

confidence: 99%

Phospholipid Bilayer-Perturbing Properties Underlying Lysis Induced by pH-Sensitive Cationic Lysine-Based Surfactants in Biomembranes

Nogueira¹,

Mitjans

Busquets³

et al. 2012

Langmuir

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Amino acid-based surfactants constitute an important class of natural surface-active biomolecules with unpredictable number of industrial applications. To gain better mechanistic understanding of surfactant-induced membrane destabilization, we assessed the phospholipid bilayer-perturbing properties of new cationic lysine-based surfactants. We used erythrocytes as biomembrane models to study the hemolytic activity of surfactants and their effects on cells' osmotic resistance and morphology, as well as on membrane fluidity and membrane protein profile with varying pH. The antihemolytic capacity of amphiphiles correlated negatively with the length of the alkyl chain.Anisotropy measurements showed that the pH-sensitive surfactants, with the positive charge on the α-amino group of lysine, significantly increased membrane fluidity at acidic conditions. SDS-PAGE analysis revealed that surfactants induced significant degradation of membrane proteins in hypo-osmotic medium and at pH 5.4. By scanning electron microscopy examinations, we corroborated the interaction of surfactants with lipid bilayer. We found that varying the surfactant chemical structure is a way to modulate the positioning of the molecule inside bilayer and, thus, the overall effect on the membrane. Our work showed that pH-sensitive lysine-based surfactants significantly disturb the lipid bilayer of biomembranes especially at acidic conditions, which suggests that these compounds are promissing as a new class of multifunctional bioactive excipients for active intracellular drug delivery.

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“…The viability of acute myeloid leukemia cell lines, including U937, Kasumi-1, HL-60, and NB4 cells, was considerably decreased after treatment with puerarin via inhibition of the cell cycle. Further studies confirmed the role of puerarin in the reduction of motility migration, adhesion, and invasion of human HO-8910 omophoria cells [19]. More recently, puerarin has been shown to decrease the apoptosis of retinal pigment epithelial cells by preventing damage in peroxynitrites [20].…”

Section: Discussionmentioning

confidence: 71%

Inhibitory effect of puerarin on proliferation of retinoblastoma cells: An <i>in-vitro</i> study

Zhan¹,

Zhu²,

Zhang³

2017

Trop. J. Pharm Res

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Purpose: To investigate the anti-proliferative effect of puerarin on retinoblastoma cells. Methods: The effect of puerarin was examined on human retinoblastoma Y79 cells using cell proliferation assays and reverse transcription-polymerase chain reaction (RT-PCR). The effect of puerarin on the cell cycle was also investigated. Western blot and RT-PCR analyses were also performed to identify the putative mechanism of action. Results: The results showed that cell viability was suppressed by puerarin in a concentrationdependent manner with a half-maximal inhibitory concentration (IC50)

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Role of the interaction between puerarin and the erythrocyte membrane in puerarin-induced hemolysis

Cited by 35 publications

References 37 publications

Assessment of Cytotoxicity Profiles of Different Phytochemicals: Comparison of Neutral Red and MTT Assays in Different Cells in Different Time Periods

Assessment of Cytotoxicity Profiles of Different Phytochemicals: Comparison of Neutral Red and MTT Assays in Different Cells in Different Time Periods

Phospholipid Bilayer-Perturbing Properties Underlying Lysis Induced by pH-Sensitive Cationic Lysine-Based Surfactants in Biomembranes

Inhibitory effect of puerarin on proliferation of retinoblastoma cells: An <i>in-vitro</i> study

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