Role of the humoral immune system in Salmonella enteritidis phage type four infection in chickens

Desmidt, Maria; Ducatelle, Richard; Mast, Jan; Goddeeris, Bruno; Kaspers, Bernd; Haesebrouck, Freddy

doi:10.1016/s0165-2427(98)00112-3

Cited by 71 publications

(44 citation statements)

References 24 publications

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“…However, secretory IgA responses which are thought to play a central role in mucosal surface protection cannot be elicited by killed Salmonellae vaccine [57,58].…”

Section: Atmentioning

confidence: 99%

“…In chickens vaccination may reduce the severity and period of infection and help avoid re-infection [59], which indirectly should reduce the number of human food-borne salmonellosis cases [60]. It has been shown that inactivated vaccines of S. Enteritidis can induce a good humoral immune response, and induce some reduction in intestinal colonization by S. Enteritidis [57,[61][62][63].…”

Section: Atmentioning

confidence: 99%

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Untitled

2017

JMEN

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Salmonella enterica can be considered as one of the most important causes of foodpoisoning with poultry thought to be the main source. Although S. Typhimurium, S. Enteritidis and the vast majority of other Salmonella serovars generally produce little systemic disease in adult chickens, they are able to colonize the alimentary tract of poultry. The two caeca are the main sites of the colonization of Salmonellae in chickens, and the bacteria can be easily harvested from the caeca for analysis. Bacterial proteins analyses utilizing SDS-PAGE showed differences between in vitro and in vivo that out of about 40 protein bands of in vitro preparation only a few (3-5) bands can be visualized from in vivo preparations. We suggested that some avian proteases might be responsible. Accordingly, and to investigate the hypothesis that an inactivated vaccine harvested from chickens is thought to be more protective than bacteria grown in broth culture, the immunogenicity of inactivated vaccines, either proteins or formalin killed bacteria (S. Enteritidis) harvested from chicken intestine and those from broth culture (in vitro), were compared using bacterial proteins or formalin killed bacteria as an orally inoculated vaccine candidate in chicken. The results demonstrated that sonicated proteins extracts in general have a stronger effect as vaccines against S. Enteritidis colonization in chickens than formalin killed bacteria. In contrast, the in vitro sonicated proteins obtained from a nutrient broth culture had a much better protective vaccine effect than the in vivo sonicated proteins preparations harvested from bacteria grown in chickens. IntroductionMany bacterial pathogens such as Clostridium, Staphylococcus, Campylobacter and many other bacterial strains are capable of causing food-poisoning, and Salmonella enterica can be considered as one of the most important causes with poultry thought to be the main source. Although S. Typhimurium, S. Enteritidis and the vast majority of other Salmonella serovars generally produce little systemic disease in adult chickens, they are able to colonize the alimentary tract of poultry, resulting in contamination of poultry carcasses and entry into the human food chain. However, there is a great demand to control food-poisoning salmonellosis at both breeder and layer levels at the national and global level in order to produce Salmonelle-free poultry products, due to the current correlation between S. Enteritidis PT4 and poultry products. Salmonellosis costs the European Union a minimum of 500-900 million Euros annually. Salmonellosis in food animals is a major target for reduction of human infection by the European Union. Legislation has been introduced to monitor the most important Salmonellae serovars. The major Salmonellae serovars of public health consequence are S. Typhimurium and S. Enteritidis (causing 15% and 60% respectively of all cases in Europe in 2002).Elazomi and Dhawi studied newly-hatched chickens infected with S. Enteritidis. They analyzed proteins of S. Enteritidis...

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“…However, secretory IgA responses which are thought to play a central role in mucosal surface protection cannot be elicited by killed Salmonellae vaccine [57,58].…”

Section: Atmentioning

confidence: 99%

Section: Atmentioning

confidence: 99%

Untitled

2017

JMEN

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“…However, the occurrence of the clinical disease associated with mortality has been observed in birds that are submitted to stress conditions and young broiler chicks that have an immature immune system (Akthar et al, 2013). Studies based on experimental inoculation of these bacteria in the first week of life demonstrate that both S. Enteritidis and S. Typhimurium cause infections that can lead to the development of acute septicemia, characterized by the presence of lesions such as peritonitis, perihepatitis, onfalitis, pericarditis, tiflitis, aerossaculitis and pneumonia (Desmidt et al, 1997;Dhillon, et al, 2001;Roy et al, 2001). Similarly, high mortality taxes are mentioned after experimental inoculation of those serovars in one-day-old chicks (Smith and Tucker, 1980).…”

Section: Most Birds Infected By S Enteritidis and Smentioning

confidence: 99%

Establishment of a pathogenicity index in Salmonella Enteritidis and Salmonella Typhimurium strains inoculated in one-day-old broiler chicks

Lima

Furian

Pillati

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Salmonella Enteritidis and Salmonella Typhimurium are responsible for causing huge economic loses in aviculture, as they lead young broiler chicks to develop clinical disease and thus increase mortality. Salmonella's pathogenicity is considered complex and multifactorial, demanding more studies that could elucidate the interaction between host and pathogen. The present study aims to evaluate the virulence of 130S. Enteritidis isolates and 70S. Typhimurium inoculated in one-day-old chicks through the establishment of a pathogenicity index. For each strain, 10 commercial chicks from the Cobb lineage were used. Then, 200µL of a solution containing 2x10 8 CFU of S. Enteritidis or S. Typhimurium were inoculated in the birds by intraperitoneal via. Mortality and presence of lesions such as aerosaculitis (A), perihepatitis (Ph), pericarditis (Pc), peritonitis (Pt), onfalitis (O) and cellulitis (C) were registered daily for seven days. From the second to the seventh day there was a proportional decrease in the punctuation of the time of death (TD) for each day that the bird had survived. The pathogenicity index was calculated using the following formula: PI = (TD x 5) + A + Ph + Pc + Pt + O + C. The obtainment of the PI of each bacterial sample was achieved by calculating the rate of the ten inoculated birds. Based on the obtained results, it was possible to attribute the pathogenicity value for each strain, which enabled us to classify them in groups of low (27/200), intermediate (95/200) and high (78/200) pathogenicity. The utilization of standards like time of death and presence of septicemic lesions made it possible to determine the pathogenicity rate for each strain. Besides that, the proposed model has presented dramatic differences between the high, intermediate and low pathogenicity groups, which makes this mechanism useful for further classification of strains isolated in poultry farms.

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“…It is believed that both CD4+ and CD8+ T cells are required for immune clearance ) and despite the fact that on theoretical grounds, clearance from the intestine should involve primarily a humoral response there is mixed evidence that this is the major factor (Brownwell et al, 1970;Corrier et al, 1991;Desmidt et al, 1998b).…”

Section: Scientific Bases For Vaccinationmentioning

confidence: 99%

Opinion of the Scientific Panel on biological hazards (BIOHAZ) related to the use of vaccines for the control of Salmonella in poultry

2004

EFSA Journal

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Role of the humoral immune system in Salmonella enteritidis phage type four infection in chickens

Cited by 71 publications

References 24 publications

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Establishment of a pathogenicity index in Salmonella Enteritidis and Salmonella Typhimurium strains inoculated in one-day-old broiler chicks

Opinion of the Scientific Panel on biological hazards (BIOHAZ) related to the use of vaccines for the control of Salmonella in poultry

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