Role of the hamster ovum penetration test in infertility diagnosis

Zenzes, Maria Teresa; Kan, I.; Hano, R.; Schubeus, P.; Nieschlag, Eberhard

doi:10.1111/j.1365-2605.1986.tb00914.x

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…A positive correlation ( r = 0.78, P < 0.01) between penetration rate and the post-irradiation time was found. These rates, which ranged in our patients with seminoma from 14 to 73%, were within the normal range (15-100%) for 31 fertile men tested in our laboratory Zenzes et al, 1986). Cancer patients treated with higher testicular doses (40 to 500 rad) showed lower penetration rates (Martin et al, 1985) even 24 months after radiotherapy.…”

Section: Discussionsupporting

confidence: 67%

“…Polyspermy is assessed by the penetration index (p.i.). This parameter measures all of the penetrating sperm and gives information additional to the penetration rate, which only measures the first penetrating sperm (Zenzes et al, 1986). In the present study, a lack of polyspermy (i.e.…”

Section: Discussionmentioning

confidence: 88%

“…The average penetration index was 0.41 and ranged from 0.14 to 0.73. Controls were 31 fertile donors tested in our laboratory with an average penetration rate of 52.1+3.2% and a range of 15-100%; their mean penetration index was 0.80+0.08 with a range of 0.15-1.80 (Zenzes et al, 1986). There was no significant difference in penetration rate comparing these two groups.…”

Section: Hamster Ovum Penetration Testmentioning

confidence: 99%

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Testicular function in eight patients with seminoma after unilateral orchidectomy and radiotherapy

et al. 1987

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Testicular function was monitored in eight patients with low stage seminoma who were treated with radiotherapy following unilateral orchidectomy. The absorbed gonadal radiation dose ranged from 15 to 157.5 rad. At 10-24 months after radiotherapy, serum hormone levels, sperm analysis, sperm penetration into zona-free hamster ova (HOP-test) and lymphocyte chromosome abnormalities were evaluated. Two patients were azoospermic with elevated serum levels of LH and FSH. The remaining six patients had slightly decreased (n = 3) or normal (n = 3) seminal parameters. Their HOP rates were within the normal range. A low incidence of polyspermy (i.e. only one penetrating sperm per ovum) was found in the patients, suggesting low penetrability of motile sperm. A highly significant correlation was found between sperm count or sperm penetrability and time post-irradiation. The results indicate that restitution of testicular function is time-dependent.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 88%

Section: Hamster Ovum Penetration Testmentioning

confidence: 99%

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Testicular function in eight patients with seminoma after unilateral orchidectomy and radiotherapy

et al. 1987

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“…Results from such groups have also been used to compare the semen parameters of an infertile population (MacLeod & Gold,195 1 a & b). Furthermore "fertile men" have been used to set standards for biological sperm function tests, such as the zona-free hamster egg penetration test (Peter et al, 1986;Zenzes et al, 1986). Surprisingly there are not standardised criteria for the recruitment of these men.…”

Section: Introductionmentioning

confidence: 99%

The importance of employing stringent methods to recruit fertile male controls

et al. 2009

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Two methods of recruiting fertile male controls were evaluated and compared. The first group was recruited from the partners of women attending an antenatal clinic without obtaining details of their reproductive history. The second group was recruited after obtaining a detailed reproductive history from the couple and employing stringent entry criteria. Entry criteria for the second group included a length of exposure to the risk of pregnancy of not more than 12 months and no previous episode of involuntary infertility for either partner. There were significant differences between the distributions of semen parameters obtained from the two groups, indicating that the selection criteria for "fertile" men significantly influence results obtained and therefore that it is important to employ stringent criteria for the recruitment of fertile male controls. The group which was recruited by stringent criteria (mean length of exposure to the risk of pregnancy of 3 months) was characterised by a significantly higher median concentration of spermatozoa which exhibited slow linear or nonlinear motility. This confirms the findings of a previous study which suggested that slow linear or nonlinear motility are superior forms of spermatozoal motion.

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