Role of the Halloween genes, Spook and Phantom in ecdysteroidogenesis in the desert locust, Schistocerca gregaria

Marchal, Elisabeth; Badisco, Liesbeth; Verlinden, Heleen; Vandersmissen, Tim; Soest, Sofie Van; Wielendaele, Pieter Van; Broeck, Jozef Vanden

doi:10.1016/j.jinsphys.2011.05.009

Cited by 82 publications

(94 citation statements)

References 37 publications

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“…After oviposition, these pots were collected once a week and set apart in empty cages, resulting in pools of hatched first instar hoppers, which differed by no more than 7 days in age. Depending on the experimental conditions, the locusts were further synchronized at the time of ecdysis (Badisco et al, 2011a; Marchal et al, 2011). …”

Section: Methodsmentioning

confidence: 99%

Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria

et al. 2015

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Allatotropins (ATs) are pleiotropic neuropeptides initially isolated from the tobacco hornworm, Manduca sexta. In 2008, the first receptor for AT-like peptides (ATR) was characterized in Bombyx mori. Since then, ATRs have also been characterized in M. sexta, Tribolium castaneum, Aedes aegypti and Bombus terrestris. These receptors show sequence similarity to vertebrate orexin (ORX) receptors. When generating an EST-database of the desert locust (Schistocerca gregaria) central nervous system, we found cDNA sequences encoding the Schgr-AT precursor and a fragment of its putative receptor. This receptor cDNA has now been completed and functionally expressed in mammalian cell lines. Activation of this receptor, designated as Schgr-ATR, by Schgr-AT caused an increase in intracellular calcium ions, as well as cyclic AMP (cAMP), with an EC50 value in the nanomolar range. In addition, the transcript distribution of both the Schgr-AT precursor and Schgr-ATR was investigated by means of quantitative real-time PCR. Moreover, we found more evidence for the myotropic and allatostimulatory actions of Schgr-AT in the desert locust. These data are discussed and situated in a broader context by comparison with literature data on AT and ATR in insects.

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Section: Methodsmentioning

confidence: 99%

Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria

et al. 2015

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“…Insect larvae synthesise ecdysone in the prothoracic gland although it is destined to degenerate in the adult of most species [20]. The synthetic organ in decapods is the Y-organ.…”

Section: Introductionmentioning

confidence: 99%

Roles of ecdysteroids for progression of reproductive cycle in the fresh water crustacean Daphnia magna

et al. 2014

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Introduction: Daphnia magna exhibits a parthenogenetic reproductive cycle linked to a moulting cycle, but regulatory mechanisms of neither moulting nor reproductive cycle are understood in daphnids. Moulting is regulated by ecdysteroids in insects. A previous study showed that a titre of ecdysteroids changed during the reproductive cycle in D. magna; however, no clear correlation among titre, moulting and reproductive cycles has been proved in daphnids. To understand endocrine mechanisms underlying the coordinated reproductive cycle, we analysed the expression of genes coding for enzymes in ecdysteroids synthesis or inactivation pathways, and the effects of 20-hydroxyecdysone (20E) on moulting and ovulation in D. magna.

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“…RNA interference of insulin-related peptide and neuroparsins revealed a possible role for the latter peptides in vitellogenesis (Badisco et al 2011b). The same technique also revealed a role of the Halloween genes, spook and phantom, in ecdysteroidogenesis in S. gregaria (Marchal et al 2011). In T. castaneum, it was demonstrated by RNAi experiments that the Knickkopf (Knk) protein is involved in the insensitivity of newly formed cuticle against molting enzymes and that Knk plays a role in the proper organization and layering of the exoskeletal laminae (Chaudhari et al 2011).…”

Section: Rna Interferencementioning

confidence: 85%

Insect omics research coming of age¹This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.

et al. 2012

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As more and more insect genomes are fully sequenced and annotated, omics technologies, including transcriptomic, proteomic, peptidomics, and metobolomic profiling, as well as bioinformatics, can be used to exploit this huge amount of sequence information for the study of different biological aspects of insect model organisms. Omics experiments are an elegant way to deliver candidate genes, the function of which can be further explored by genetic tools for functional inactivation or overexpression of the genes of interest. Such tools include mainly RNA interference and are currently being developed in diverse insect species. In this manuscript, we have reviewed how omics technologies were integrated and applied in insect biology.Résumé : À mesure que de plus en plus de génomes d'insectes sont complètement séquencés et annotés, les technologies « omiques », en particulier la transcriptomique, la protéomique, la peptidomique et le profilage métobolomique, de même que la bioinformatique, peuvent servir à exploiter cet immense quantité d'information séquencée pour l'étude de différents aspects biologiques des organismes modèles entomologiques. Les expériences omiques représentent une voie élégante pour fournir des gènes candidats dont la fonction peut être encore explorée plus avant à l'aide des outils génétiques d'inactivation fonctionnelle ou de surexpression des gènes retenus. Ces outils comprennent surtout l'ARN interférent et sont en train d'être mis au point pour diverses espèces d'insectes. Nous passons en revue dans notre présentation comment les technologies omiques ont été intégrées dans la biologie des insectes et comment on les a utilisées.

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Role of the Halloween genes, Spook and Phantom in ecdysteroidogenesis in the desert locust, Schistocerca gregaria

Cited by 82 publications

References 37 publications

Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria

Molecular cloning and characterization of the allatotropin precursor and receptor in the desert locust, Schistocerca gregaria

Roles of ecdysteroids for progression of reproductive cycle in the fresh water crustacean Daphnia magna

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