Role of the H subunit C-terminal domain in the assembly of the vacuolar H+-ATPase

Sharma, Stuti; Ra, Oot; Wilkens, Stephan

doi:10.1101/391656

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“…Helicoverpa armigera (Lepidoptera: Noctuidae; Hübner) is a devastating pest of many important crop plants throughout the world and thus responsible for heavy economic losses. 18 This pest feeds actively on various plants for development and progression into the reproductive adult phase. However, current strategies for the control of H. armigera have relied heavily on conventional chemical control, resulting in the development of resistance in H. armigera to almost all of the insecticides (; ).…”

Section: Introductionmentioning

confidence: 99%

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Azadirachtin-A from Azadirachta indica Impacts Multiple Biological Targets in Cotton Bollworm Helicoverpa armigera

et al. 2019

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Azadirachtin-A (AzaA) from the Indian neem tree ( Azadirachta indica ) has insecticidal properties; however, its molecular mechanism remains elusive. The “targeted and nontargeted proteomic profiling”, metabolomics, matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) imaging, gene expression, and in silico analysis provided clues about its action on Helicoverpa armigera . Fourth instar H. armigera larvae fed on AzaA-based diet (AzaD) suffered from significant mortality, growth retardation, reduced larval mass, complications in molting, and prolonged development. Furthermore, death of AzaD-fed larvae was observed with various phenotypes like bursting, blackening, and half-molting. Liquid chromatography–mass spectrometry (LC–MS) data showed limited catabolic processing of ingested AzaA and dramatic alternations of primary metabolism in H. armigera . MALDI-TOF imaging indicated the presence of AzaA in midgut of H. armigera . In the gut, out of 79 proteins identified, 34 were upregulated, which were related to digestion, immunity, energy production, and apoptosis mechanism. On the other hand, 45 proteins were downregulated, including those from carbohydrate metabolism, lipid metabolism, and energy transfer. In the hemolymph, 21 upregulated proteins were reported to be involved in immunity, RNA processing, and mRNA-directed protein synthesis, while 7 downregulated proteins were implicated in energy transfer, hydrolysis, lipid metabolism, defense mechanisms, and amino acid storage-related functions. Subsequently, six target proteins were identified using labeled AzaA that interacted with whole insect proteins. In silico analysis suggests that AzaA could be efficiently accommodated in the hydrophobic pocket of juvenile hormone esterase and showed strong interaction with active site residues, indicating plausible targets of AzaA in H. armigera . Quantitative polymerase chain reaction analysis suggested differential gene expression patterns and partly corroborated the proteomic results. Overall, data suggest that AzaA generally targets more than one protein in H. armigera and hence could be a potent biopesticide.

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Section: Introductionmentioning

confidence: 99%

“…Helicoverpa armigera (Lepidoptera: Noctuidae; Hübner) is a devastating pest of many important crop plants throughout the world and thus responsible for heavy economic losses . This pest feeds actively on various plants for development and progression into the reproductive adult phase.…”

Section: Introductionmentioning

confidence: 99%

Azadirachtin-A from Azadirachta indica Impacts Multiple Biological Targets in Cotton Bollworm Helicoverpa armigera

et al. 2019

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Role of the H subunit C-terminal domain in the assembly of the vacuolar H+-ATPase

Abstract: The vacuolar H + -ATPase (V-ATPase) is regulated by reversible disassembly into autoinhibited V1-ATPase and Vo proton channel sectors, a process that is poorly understood on the molecular level. V-

Cited by 1 publication

References 52 publications

Azadirachtin-A from Azadirachta indica Impacts Multiple Biological Targets in Cotton Bollworm Helicoverpa armigera

Azadirachtin-A from Azadirachta indica Impacts Multiple Biological Targets in Cotton Bollworm Helicoverpa armigera

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