Role of the extracytoplasmic function sigma factor CarQ in oxidative response of Bradyrhizobium japonicum

Thaweethawakorn, Anchana; Parks, Dylan; So, Jae-Seong; Chang, Woo Suk

doi:10.1007/s12275-015-5308-9

Cited by 3 publications

(2 citation statements)

References 43 publications

(13 reference statements)

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“…viciae , perhaps due to redundant regulatory systems ( 20 ). In contrast, B. diazoefficiens ecfG mutants show severe nodulation defects because a functional GSR is critical in early symbiosis ( 71 ), a group ECF16 σ (EcfS) is required for effective symbiosis in B. diazoefficiens ( 55 ), and ECF σs (EcfF and EcfQ/CarQ) also play a prominent role in the B. diazoefficiens oxidative stress response ( 54 , 72 ).…”

Section: Resultsmentioning

confidence: 99%

MostSinorhizobium melilotiExtracytoplasmic Function Sigma Factors Control Accessory Functions

Lang

Barnett

Fisher

et al. 2018

mSphere

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Fixed (reduced) soil nitrogen plays a critical role in soil fertility and successful food growth. Much soil fertility relies on symbiotic nitrogen fixation: the bacterial partner infects the host plant roots and reduces atmospheric dinitrogen in exchange for host metabolic fuel, a process that involves complex interactions between the partners mediated by changes in gene expression in each partner. Here we test the roles of a family of 11 extracytoplasmic function (ECF) gene regulatory proteins (sigma factors [σs]) that interact with RNA polymerase to determine if they play a significant role in establishing a nitrogen-fixing symbiosis or in responding to various stresses, including cell envelope stress. We discovered that symbiotic nitrogen fixation occurs even when all 11 of these regulatory genes are deleted, that most ECF sigma factors control accessory functions, and that none of the ECF sigma factors are required to survive envelope stress.

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Section: Resultsmentioning

confidence: 99%

MostSinorhizobium melilotiExtracytoplasmic Function Sigma Factors Control Accessory Functions

Lang

Barnett

Fisher

et al. 2018

mSphere

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“…The association between ECF and oxidative stress adaptation has been widely reported in several microorganisms, such as Porphyromonas gingivalis (σ E ), Bacteroides fragilis (EcfO), Bradyrhizobium japonicum (CarQ), and Shewanella oneidensis (σ E2 ) ( 13 , 48 – 50 ). ECF acts as a positive regulator that protects bacteria from oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Implication of the σ ^E Regulon Members OmpO and σ ^N in the Δ ompA _299–356 -Mediated Decrease of Oxidative Stress Tolerance in Stenotrophomonas maltophilia

Liu

et al. 2023

Microbiol Spectr

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Identification and Validation of Reference Genes for Expression Analysis in Nitrogen-Fixing Bacteria under Environmental Stress

Parks

Peterson

Chang

2022

Life

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Reference genes, also referred to as housekeeping genes (HKGs), play an important role in gene expression analysis by serving as an internal control. These HKGs are usually involved in basic cellular functions and their expression should remain at relatively constant levels. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) has been used to measure gene expression. Since the normalization of gene expression data depends on baseline expression of HKGs, it is important to identify and verify true HKGs for the qRT-PCR analysis. The goal of this study is to identify and confirm HKGs in Bradyrhizobium diazoefficiens, a nitrogen fixing bacterium which forms a symbiotic relationship with soybean. By revealing such HKGs, the normalization of gene expression would be more robust, reliable, and consistent. Here, we analyzed previous gene expression data for B. diazoefficiens under multiple environmental conditions. As a result, we identified seven constitutively expressed genes among 8453 genes across all conditions. Their fold-change values were within a range of −1.25-fold < x < 1.25-fold. We adopted GeNorm, NormFinder, and comparative ∆Ct methods to rank the seven candidate genes based on their expression stability. To validate these potential HKGs, we measured their expression in various experimental conditions, such as heat, pH, and heavy metal stress. The HKGs that were found in B. diazoefficiens were also applied in closely related species by identifying their homologs.

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Role of the extracytoplasmic function sigma factor CarQ in oxidative response of Bradyrhizobium japonicum

Cited by 3 publications

References 43 publications

MostSinorhizobium melilotiExtracytoplasmic Function Sigma Factors Control Accessory Functions

MostSinorhizobium melilotiExtracytoplasmic Function Sigma Factors Control Accessory Functions

Implication of the σ ^E Regulon Members OmpO and σ ^N in the Δ ompA _299–356 -Mediated Decrease of Oxidative Stress Tolerance in Stenotrophomonas maltophilia

Identification and Validation of Reference Genes for Expression Analysis in Nitrogen-Fixing Bacteria under Environmental Stress

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Role of the extracytoplasmic function sigma factor CarQ in oxidative response of Bradyrhizobium japonicum

Cited by 3 publications

References 43 publications

MostSinorhizobium melilotiExtracytoplasmic Function Sigma Factors Control Accessory Functions

MostSinorhizobium melilotiExtracytoplasmic Function Sigma Factors Control Accessory Functions

Implication of the σ E Regulon Members OmpO and σ N in the Δ ompA 299–356 -Mediated Decrease of Oxidative Stress Tolerance in Stenotrophomonas maltophilia

Identification and Validation of Reference Genes for Expression Analysis in Nitrogen-Fixing Bacteria under Environmental Stress

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Product

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Implication of the σ ^E Regulon Members OmpO and σ ^N in the Δ ompA _299–356 -Mediated Decrease of Oxidative Stress Tolerance in Stenotrophomonas maltophilia