Role of the expansin genes NtEXPA1 and NtEXPA4 in the regulation of cell extension during tobacco leaf growth

Kuluev, B. R.; Kniazev, Andrey; Никоноров, Ю М; Чемерис, А. В.

doi:10.1134/s1022795414040061

Cited by 29 publications

(21 citation statements)

References 22 publications

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“…To date, a large number of expansin gene families has been identified and characterized in both model and crop plants, including Arabidopsis, rice and maize, to mention but a few. However, no such kind of analysis has been done in tobacco, except expression studies for a few NtEXPs genes (Kuluev et al 2013(Kuluev et al , 2014. Therefore, it is of great importance to identify the expansin gene family in the tobacco genome and explore the characteristics and functions of each expansin gene.…”

Section: Discussionmentioning

confidence: 99%

“…They are considered to bind to glucan-coated cellulose in the cell wall causing a reversible disruption of hydrogen bonding between cellulose microfibrils and glucan matrix and thus loosening the cell wall in a pH-dependent manner Cosgrove 2015). Expansins are commonly found in plants and are well known to play important roles in diverse biological processes related to cell wall modification, including seed germination , root growth and development Yu et al 2011), stem growth and internode elongation (Cho and Kende 1997;Kuluev et al 2014), leaf initiation and expansion (Fleming et al 1997;Kuluev et al 2013), flowering and flower size (Kuluev et al 2012), pollen germination and fertilization (Cosgrove 2000a), fruit growth and/or ripening (Rose et al 1997;Brummell et al 1999;Civello et al 1999;Ishimaru et al 2007). They have also been associated with nutrient uptake and efficiency (Zhou et al 2014), abiotic and biotic stress tolerance Zhao et al 2011;Lü et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Although genome-wide studies on expansins have been reported in many plant species, a systematic analysis of this gene family in tobacco (Nicotiana tabacum) has not yet been published to date, except expression studies for a few tobacco expansin genes (Kuluev et al 2013(Kuluev et al , 2014. The completion of high-quality draft genomes of tobacco (Sierro et al 2014) provides us the opportunity to uncover the expansin gene family and its characteristics in this allotetraploid crop.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum)

2016

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Expansins are pH-dependent cell wall loosening proteins which form a large family in plants. They have been shown to be involved in various developmental processes and been implicated in enabling plants' ability to absorb nutrients from the soil as well as conferring biotic and abiotic stress resistances. It is therefore clear that they can be potential targets in genetic engineering for crop improvement. Tobacco (Nicotiana tabacum) is a major crop species as well as a model organism. Considering that only a few tobacco expansins have been studied, a genome-wide analysis of the tobacco expansin gene family is necessary. In this study, we identified 52 expansins in tobacco, which were classified into four subfamilies: 36 NtEXPAs, 6 NtEXPBs, 3 NtEXLAs and 7 NtEXLBs. Compared to other species, the NtEXLB subfamily size was relatively larger. Phylogenetic analysis showed that the 52 tobacco expansins were divided into 13 subgroups. Gene structure analysis revealed that genes within subfamilies/subgroups exhibited similar characteristics such as gene structure and protein motif arrangement. Whole-genome duplication and tandem duplication events may have played important roles in the expanding of tobacco expansins. Cis-Acting element analysis revealed that each expansin gene was regulated or several expansin genes were co-regulated by both internal and environmental factors. 35 of these genes were identified as being expressed according to a microarray analysis. In contrast to most NtEXPAs which had higher expression levels in young organs, NtEXLAs and NtEXLBs were preferentially expressed in mature or senescent tissues, suggesting that they might play different roles in different organs or at different developmental stages. As the first step towards genome-wide analysis of the tobacco expansin gene family, our work provides solid background information related to structure, evolution and expression as well as regulatory cis-acting elements of the tobacco expansins. This information will provide a strong foundation for cloning and functional exploration of expansin genes in tobacco.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum)

2016

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“…Recent examples include the diverse expression of EXPB genes in soybean, particularly during Rhizobium infection and mycorrhiza associations [53], the expression of expansin genes in the wheat peduncle with modulation by Rht alleles for dwarfism, the involvement of multiple expansin genes in Arabidopsis leaf growth [54], and the complex regulation of expansin gene expression in grape during berry ripening and during phylloxera-induced gall formation [17,55]. Building on earlier studies (reviewed in [56]), recent work has also documented expression of specific expansin genes in response to various hormones [57–60]. Wherever cells are growing or modifying their cell walls, one or more expansin genes are usually involved.…”

Section: Developmental Expressionmentioning

confidence: 99%

Plant expansins: diversity and interactions with plant cell walls

Cosgrove

2015

Current Opinion in Plant Biology

373

370

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Expansins were discovered two decades ago as cell wall proteins that mediate acid-induced growth by catalyzing loosening of plant cell walls without lysis of wall polymers. In the interim our understanding of expansins has gotten more complex through bioinformatic analysis of expansin distribution and evolution, as well as through expression analysis, dissection of the upstream transcription factors regulating expression, and identification of additional classes of expansin by sequence and structural similarities. Molecular analyses of expansins from bacteria have identified residues essential for wall loosening activity and clarified the bifunctional nature of expansin binding to complex cell walls. Transgenic modulation of expansin expression modifies growth and stress physiology of plants, but not always in predictable and even understandable ways.

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“…It means that new leaf primordia appear in the places with local weakening of cell walls, which can be caused not only by expansins, but also xyloglucan endotransglucosylases (Miedes et al 2010). In literature there is information about ARGOS gene homologs overexpression being able to induce expression of both expansins (Wang et al 2009a, Kuluev et al 2013) and xyloglucan endotransglucosylases (Hu et al 2006, Kuluev et al 2014. So it can be suggested that overexpression of PnARGOS-LIKE gene also induces the expression of certain proteins, influencing the weakening of the cell wall.…”

Section: Resultsmentioning

confidence: 94%

The poplar ARGOS-LIKE gene promotes leaf initiation and cell expansion, and controls organ size

Kuluev¹,

Knyazev²,

Mikhaylova³

et al. 2016

Biol. plant.

Self Cite

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We identified Populus nigra auxin-regulated gene involved in organ size (PnARGOS)-LIKE encoding one of organ size related (OSR)-proteins in black poplar which is homologous to AtARGOS and AtARGOS-LIKE genes of Arabidopsis thaliana. ABRE-like, G-box, GATA and Ibox motifs were discovered in the promoter region of poplar ARGOS-LIKE gene. In wild type aspen (Populus tremula) plants, ortholog of PnARGOS-LIKE gene (PtrARGOS-LIKE) was noticeably expressed in the actively dividing and expanding young leaves and calli, whereas its mRNA content increased in response to exogenous 6-benzylaminopurine, 1-naphthaleneacetic acid, and 24-epibrassinolide. The expression of PtrARGOS-LIKE gene was reduced under salinity treatment. In addition, we generated transgenic tobacco and aspen plants with up-regulated expression of PnARGOS-LIKE gene. Constitutive expression of the gene contributed to an increase in size of stems and leaves of transgenic tobacco plants. In transgenic aspen constitutive expression of PnARGOS-LIKE gene promoted an increase in the frequency of leaf initiation and in the leaf length and area. The size of transgenic tobacco and aspen leaves increased due to the enlargement of individual cells. The results show the significance of PnARGOS-LIKE gene for the control of leaf initiation and organ growth by cell expansion in poplar.

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Role of the expansin genes NtEXPA1 and NtEXPA4 in the regulation of cell extension during tobacco leaf growth

Cited by 29 publications

References 22 publications

Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum)

Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum)

Plant expansins: diversity and interactions with plant cell walls

The poplar ARGOS-LIKE gene promotes leaf initiation and cell expansion, and controls organ size

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