Role of the Endothelin-1 System in the Luteolytic Process of Pseudopregnant Rabbits

Boiti, Cristiano; Guelfi, Gabriella; Brecchia, Gabriele; Dall’Aglio, Cecilia; Ceccarelli, Piero; Maranesi, Margherita; Mariottini, Chiara; Zampini, Danilo; Gobbetti, Anna; Zerani, Massimo

doi:10.1210/en.2004-1099

Cited by 34 publications

(41 citation statements)

References 46 publications

(67 reference statements)

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“…Several studies have indicated a role for NO in luteolysis, because NO inhibited progesterone secretion in cows and rats (Motta & Gimeno 1997, Skarzynski et al 2005. It has been suggested that during luteolysis NO may interact with such luteolytic factors as PGF 2a or endothelin 1 (Tognetti et al 2003, Boiti et al 2005. However, it has been demonstrated that NO can reverse PGF 2a -induced inhibition of progesterone secretion by rat and sheep CL, which suggests an anti-luteolytic role for NO (Dong et al 1999, Weems et al 2005.…”

Section: Discussionmentioning

confidence: 99%

Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle

Grazul-Bilska¹,

Navanukraw²,

Johnson³

et al. 2006

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This study was conducted to evaluate the expression of endothelial nitric oxide synthase (eNOS) in ovarian follicles and corpora lutea (CL) throughout the estrous cycle in sheep. Three experiments were conducted to (1) immunolocalize eNOS protein, (2) determine expression of mRNA for eNOS and its receptor guanylate cyclase 1 soluble b3 (GUCY1B3), and (3) co-localize eNOS and vascular endothelial growth factor (VEGF) proteins in the follicles and/or CL throughout the estrous cycle. In experiment 1, ovaries were collected from ewes treated with FSH, to induce follicular growth or atresia. In experiment 2, ovaries were collected from ewes treated with FSH and hCG to induce follicular growth and ovulation. In experiment 3, ovaries were collected from superovulated ewes to generate multiple CL on days 2, 4, 10, and 15 of the estrous cycle. In experiments 1 and 2, the expression of eNOS protein was detected in the blood vessels of the theca externa and interna of healthy ovarian follicles. However, in early and advanced atretic follicles, eNOS protein expression was absent or reduced. During the immediate postovulatory period, eNOS protein expression was detected in thecal-derived cells that appeared to be invading the granulosa layer. Expression of eNOS mRNA tended to increase in granulosa cells at 12 and 24 h, and in theca cells 48 h after hCG injection. In experiment 3, eNOS protein was located in the blood vessels of the CL during the estrous cycle. Dual localization of eNOS and VEGF proteins in the CL demonstrated that both were found in the blood vessels.

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Section: Discussionmentioning

confidence: 99%

Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle

Grazul-Bilska¹,

Navanukraw²,

Johnson³

et al. 2006

Reproduction

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“…This result can be explained by two possibilities. One is the low transfection rate and the other is that PGE2 stimulation may affect MUC8 gene expression not only through the MAP kinase pathway but also through other pathways, such as G-protein-activated pathway (32) and phospholipase C pathway (33).…”

Section: Discussionmentioning

confidence: 99%

Prostaglandin E2 Induces MUC8 Gene Expression via a Mechanism Involving ERK MAPK/RSK1/cAMP Response Element Binding Protein Activation in Human Airway Epithelial Cells

Cho

Choi

Kim

et al. 2005

Journal of Biological Chemistry

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MUC8 gene expression is overexpressed in nasal polyp epithelium and is also increased by treatment with inflammatory mediators in nasal epithelial cells. These data suggest that MUC8 may be one of important mucin genes expressed in human airway. However, the mechanisms of various inflammatory mediator-induced MUC8 gene expression in normal nasal epithelial cells remain unclear. We examined the mechanism by which prostaglandin E 2 (PGE2), an arachidonic acid metabolite, increases MUC8 gene expression levels. Here, we show that ERK mitogen-activated protein kinase is essential for PGE2-induced MUC8 gene expression in normal human nasal epithelial cells and that p90 ribosomal S 6 protein kinase 1 (RSK1) mediates the PGE2-induced phosphorylation of cAMP-response element binding protein. Our results also indicate that cAMP-response element at the ؊803 region of the MUC8 promoter is an important site of PGE2-induced MUC8 gene expression. In conclusion, this study gives insights into the molecular mechanism of PGE2-induced MUC8 gene expression in human airway epithelial cells.

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“…Total RNA was extracted from CL of three rabbits for each luteal stage as described previously (Boiti et al 2005). According to the protocol provided by the manufacturer, 5 mg total RNA were reverse transcribed in 20 ml of iSCRIPT cDNA using random hexamer.…”

Section: Gnrhr-i Fshr and Lhr Real-time Rt-pcrmentioning

confidence: 99%

Expression of type I GNRH receptor and in vivo and in vitro GNRH-I effects in corpora lutea of pseudopregnant rabbits

Zerani¹,

Parillo²,

Brecchia³

et al. 2010

Journal of Endocrinology

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The expression of type I GNRH receptor (GNRHR-I) and the direct role of GNRH-I on corpora lutea (CL) function were studied in the pseudopregnant rabbit model. Immunohistochemistry evidenced GNRHR-I and GNRH-I in luteal cells at early (day 4 pseudopregnancy)-, mid (day 9)-, and late (day 13)-luteal stages. Real-time RT-PCR and western blotting revealed GNRHR-I mRNA and protein at the three luteal stages. Buserelin in vivo treatment at days 9 and 13 decreased plasma progesterone levels for 48 and 24 h respectively. In in vitro cultured CL, buserelin reduced progesterone secretion, increased prostaglandin F 2a (PGF 2a ) secretion and cyclooxygenase-2 (COX-2) and nitric oxide synthase (NOS) activities at days 9 and 13, and decreased PGE 2 at day 13. Co-incubation with antagonists for GNRH-I (antide), inositol 1,4,5-trisphosphate (IP 3 , 2-amino-ethoxydiphenylborate), and diacylglycerol (DAG, 1-hexadecyl-2-acetyl glycerol) or inhibitors for phospholipase C (PLC, compound 48/80), and protein kinase C (PKC, staurosporine) counteracted the buserelin effects. Buserelin co-incubated with COX inhibitor (acetylsalicylic acid) increased progesterone and decreased PGF 2a and NOS activity at days 9 and 13, whereas co-incubation with NOS inhibitor (N-nitro-L-arginine methyl ester) increased progesterone at the same luteal stages. These results suggest that GNRHR-I is constitutively expressed in rabbit CL independently of luteal stage, whereas GNRH-I down-regulates directly CL progesterone production via PGF 2a at mid-and late-luteal stages of pseudopregnancy, utilizing its cognate type I receptor with a post-receptorial mechanism that involves PLC, IP 3 , DAG, PKC, COX-2, and NOS.

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Role of the Endothelin-1 System in the Luteolytic Process of Pseudopregnant Rabbits

Cited by 34 publications

References 46 publications

Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle

Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle

Prostaglandin E2 Induces MUC8 Gene Expression via a Mechanism Involving ERK MAPK/RSK1/cAMP Response Element Binding Protein Activation in Human Airway Epithelial Cells

Expression of type I GNRH receptor and in vivo and in vitro GNRH-I effects in corpora lutea of pseudopregnant rabbits

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