Role of the embryo number in the development of in-vitro produced bovine embryos

Palma, G.A.; Clement-Sengewald, A.; Berg, U.; Brem, Gottfried

doi:10.1016/0093-691x(92)90340-w

Cited by 22 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although embryos fertilized in vitro are capable of developing into normal healthy offspring, these finding suggest that the optimal environment for gamete maturation, fertilization and embryonic development is important for quality of embryos derived in vitro. Zygotes cultured individually result in inferior development compared to the ones co-cultured with adequate numbers of embryos, suggesting that for optimum development embryos need some undefined paracrine factors that are absent from the culture medium (Palma et al 1992;Ferry et al 1994;O'Doherty et al 1997). Zygotes cultured individually result in inferior development compared to the ones co-cultured with adequate numbers of embryos, suggesting that for optimum development embryos need some undefined paracrine factors that are absent from the culture medium (Palma et al 1992;Ferry et al 1994;O'Doherty et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Effect of co‐culture with intact embryos on development of bovine separated blastomeres

Nagai¹,

Hori²,

Miyamoto³

et al. 2013

Animal Science Journal

View full text Add to dashboard Cite

To improve embryo development in bovine separated blastomeres, we evaluated applicability of co-culture with intact embryos. The morphological quality of blastocysts derived from separated blastomeres and rate of blastocyst formation were only slightly increased when the cells were co-cultured with intact embryos, which did not provide significant differences when statistically analyzed. However, the cell count of inner cell mass (ICM), trophectoderm (TE) and total number of cells in Day 8 blastocysts were significantly higher when the cells were co-cultured with the intact embryos than those with the cells cultured individually (P<0.05). Transfer of four monozygotic pairs of blastocysts derived from the cells co-cultured with intact embryos led to three pregnancies even when the blastomeres were produced by in vitro maturation and in vitro fertilization of oocytes collected by ovum pick-up from elite cows. These results suggest that co-culturing with intact embryos may enhance development of bovine separated blastomere.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of co‐culture with intact embryos on development of bovine separated blastomeres

Nagai¹,

Hori²,

Miyamoto³

et al. 2013

Animal Science Journal

View full text Add to dashboard Cite

show abstract

“…Embryos cultured in groups may secrete such factors in the medium, creating a suitable microenvironment for developing embryos. Palma et al (1992) demonstrated that, after group maturation and fertilization, the number of embryos placed in development influenced the rate of embryos that reached the blastocyst stage, so that the more embryos were cultured together, the greater the percentage of blastocysts obtained. Grouping the oocytes possibly rescued those that would not have developed if cultured individually because of the presence of other oocytes (of the same class) that were developmentally competent.…”

Section: Discussionmentioning

confidence: 99%

Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes

Blondin

Sirard

1995

Molecular Reproduction Devel

418

249

View full text Add to dashboard Cite

Follicular size, follicular atresia, and oocyte morphology were investigated for the possible relation of these characteristics to the developmental competence of bovine oocytes. Ovaries from a local slaughterhouse were dissected to obtain a heterogeneous population of follicles. Half of each follicle was fixed for histological analysis, and the oocytes were detached carefully and cultured individually. Before in vitro maturation, the oocytes were grouped into six different classes based on the morphology of the cumulus and the ooplasm: classes 1 and 2 represent oocytes with a homogeneous ooplasm plus a compact and complete cumulus, and classes 3-6 represent oocytes with a granulated ooplasm and an incomplete and/or expanded cumulus. Oocytes from class 3 (beginning of expansion in outer cumulus layers and slight granulations in the ooplasm) developed past the 16-cell stage significantly (P < 0.05) more than oocytes with a compact and complete cumulus (classes 1 and 2) and oocytes from classes 4-6 (incomplete and/or expanded cumulus) after 5 days of in vitro culture. Oocytes from follicles measuring 3 mm or less did not develop past the 16-cell stage, whereas follicles of 3-5 mm and 5 mm or larger developed at similar rates (17% and 21% morulae, respectively). The state of the follicle did not affect whether an embryo reached at least the 16-cell stage, as comparable rates were obtained in all three groups of follicles: nonatretic (20%), intermediate (14%), and slightly atretic (16%).(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

“…However, no experiments were conducted in the present study to clarify the optimal embryo-culture density. Several reports have suggested optimal culture densities between 1 and 13 µl/embryo [8,9,15]. We used a culture density of 5-8 µl/embryo in the present study according to our usual OPU-IVF and culture protocol.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies in mice [4, 5], sheep [6], and cattle [7][8][9] have demonstrated that group cultures promote embryo development compared with single-culture or co-culture with small numbers of embryos. In murine embryos, the blastocyst formation rate is promoted by an increase of embryo density in culture drops [10], suggesting that specific factor(s) are secreted from the embryos to influence embryo growth and development in an autocrine or paracrine manner.…”

Section: Introductionmentioning

confidence: 99%

“…In our field data, the blastocyst formation rate decreased when the number of in vitro fertilized oocytes collected from individual heifers was less than 3. Therefore, it is important to increase the efficiency of blastocyst production from small numbers of in vitro fertilized oocytes collected by OPU.Previous studies in mice [4, 5], sheep [6], and cattle [7][8][9] have demonstrated that group cultures promote embryo development compared with single-culture or co-culture with small numbers of embryos. In murine embryos, the blastocyst formation rate is promoted by an increase of embryo density in culture drops [10], suggesting that specific factor(s) are secreted from the embryos to influence embryo growth and development in an autocrine or paracrine manner.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effect of Group Culture and Embryo-culture Conditioned Medium on Development of Bovine Embryos

Fujita

Umeki

Shimura

et al. 2006

Journal of Reproduction and Development

View full text Add to dashboard Cite

Abstract.We investigated the effect of group culture on bovine embryo development, and also investigated the effect of embryo-culture conditioned medium on developmental competence of individually cultured bovine embryos. Slaughterhouse-derived bovine oocytes were matured and fertilized in vitro. The presumptive zygotes were cultured individually or cultured in groups of 2 to 5 embryos with a constant culture density (5 µl/embryo). After 7 days of culture, the rates of embryos developed to the blastocyst stage were significantly higher (P<0.05) in group cultures of more than 3 embryos/drop than for embryo culture of 1 or 2 embryos/drop. These results suggest a beneficial effect of group culture may be exerted by possible growth promoting factors secreted by embryos. In the next experiment, we investigated the effect of timing of fresh medium replacement on the development of embryos cultured in groups. The blastocyst formation rate was lower when culture medium was replaced freshly on days 2-4 after fertilization than on days 5-6. The blastocyst formation rates of single-cultured embryos were significantly (p<0.05) increased by the addition of conditioned medium derived from multiple-embryo culture. These results indicate that group culture promotes embryo development and that embryo culture-derived conditioned medium is effective for supporting development of single cultured embryos. Key words: Bovine, Conditioned medium, Development, Embryo, Single embryo (J. Reprod. Dev. 52: [137][138][139][140][141][142] 2006) ecently, ultrasound-guided ovum pick-up (OPU) has enabled repeat collection of oocytes from live cattle for genetic improvement [1][2][3]. However, the efficiency of embryo production by OPU is still low because of the limited number of oocytes collected and used for in vitro fertilization (IVF) and culture (IVC). In our field data, the blastocyst formation rate decreased when the number of in vitro fertilized oocytes collected from individual heifers was less than 3. Therefore, it is important to increase the efficiency of blastocyst production from small numbers of in vitro fertilized oocytes collected by OPU.Previous studies in mice [4, 5], sheep [6], and cattle [7][8][9] have demonstrated that group cultures promote embryo development compared with single-culture or co-culture with small numbers of embryos. In murine embryos, the blastocyst formation rate is promoted by an increase of embryo density in culture drops [10], suggesting that specific factor(s) are secreted from the embryos to influence embryo growth and development in an autocrine or paracrine manner. In the non-coculture system, embryo development is promoted by adding conditioned medium derived from bovine oviductal epithelial cells [11,12], buffalo/rat hepatocytes [13], bovine granulosa cells, or Vero

show abstract

Role of the embryo number in the development of in-vitro produced bovine embryos

Cited by 22 publications

References 0 publications

Effect of co‐culture with intact embryos on development of bovine separated blastomeres

Effect of co‐culture with intact embryos on development of bovine separated blastomeres

Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes

Effect of Group Culture and Embryo-culture Conditioned Medium on Development of Bovine Embryos

Contact Info

Product

Resources

About