Role of the Drosophila genome in Sigma virus multiplication I. Role of the ret(2)P gene; Selection of host-adapted mutants at the nonpermissive allele Pp

Contamine, Didier

doi:10.1016/0042-6822(81)90227-0

Cited by 20 publications

(27 citation statements)

References 10 publications

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“…According to this hypothesis, in a fly population infected by a virus sensitive to a restrictive allele, the frequency of restrictive alleles would increase and the frequency of the sensitive virus decrease until the fly population is cured. It is worth noting that even if a viral clone multiplies in homozygous permissive flies, the resulting viral population contains spontaneous viral mutants able to multiply efficiently in restrictive flies (Contamine 1981). In this case, it should be possible to isolate viral strains displaying a different sensitivity to a particular restrictive allele.…”

Section: Discussionmentioning

confidence: 99%

“…Viral strains are natural strains and have been previously described (Gay 1978;Contamine 1981). The viral suspensions used were from stocks kept in 10% calf serum at À80°.…”

Section: Construction Of the Recombinant Alleles Of Ref(2)pmentioning

confidence: 99%

“…Flies that became paralyzed were considered as infected. Determination of viral titer, measurement of efficiencies of the infecting unit according to different host genotypes, as well as statistical tests, were performed as described in Contamine (1981).…”

Section: Construction Of the Recombinant Alleles Of Ref(2)pmentioning

confidence: 99%

“…Along generations, such modifications of the hereditary transmission rules would lead to a cure of infected fly populations. However, viruses that have become adapted to multiply in restrictive flies may appear in viral populations (Contamine 1981) and can eventually invade the fly population, as observed in Languedoc populations (Fleuriet and Periquet 1993).…”

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confidence: 99%

“…Two classes of alleles have been found in natural populations: permissive alleles that permit sigma virus multiplication and restrictive alleles that slow down viral multiplication. The efficiency of a particular restrictive allele in restraining the virus depends on the viral strain (Contamine 1981).…”

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confidence: 99%

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Control of Sigma Virus Multiplication by the ref(2)P Gene of Drosophila melanogaster: An in Vivo Study of the PB1 Domain of Ref(2)P

et al. 2007

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Ref(2)P has been described as one of the Drosophila proteins that interacts with the sigma virus cycle. We generated alleles to identify critical residues involved in the restrictive (inhibiting viral multiplication) or permissive (allowing viral multiplication) character of Ref (2)P. We demonstrate that permissive alleles increase the ability of the sigma virus to infect Drosophila when compared to null alleles and we confirm that restrictive alleles decrease this capacity. Moreover, we have created alleles unfunctional in viral cycling while functional for Ref(2)P fly functions. This type of allele had never been observed before and shows that fly-and virus-related activities of Ref (2)P are separable. The viral status of Ref (2)P variants is determined by the amino-terminal PB1 domain polymorphism. In addition, an isolated PB1 domain mimics virus-related functions even if it is similar to a loss of function toward fly-related activities. The evolutionary tree of the Ref(2)P PB1 domain that we could build on the basis of the natural allele sequences is in agreement with an evolution of PB1 domain due to successive transient selection waves.

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Section: Discussionmentioning

confidence: 99%

“…Viral strains are natural strains and have been previously described (Gay 1978;Contamine 1981). The viral suspensions used were from stocks kept in 10% calf serum at À80°.…”

Section: Construction Of the Recombinant Alleles Of Ref(2)pmentioning

confidence: 99%

Section: Construction Of the Recombinant Alleles Of Ref(2)pmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Control of Sigma Virus Multiplication by the ref(2)P Gene of Drosophila melanogaster: An in Vivo Study of the PB1 Domain of Ref(2)P

et al. 2007

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Rhabdovirus Genetics

Pringle¹

1987

The Rhabdoviruses

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There are five families of RNA viruses in which the negative strand is sequestered in the extracellular virion. Viruses of two of these families, the Rhabdoviridae and the Paramyxoviridae, have unitary linear genomes, whereas viruses of the other three families, Arenaviridae, Bunyaviridae, and Orthomyxoviridae, have segmented genomes comprising, respectively, two, three, and seven or eight subunits. The informational macromolecules that comprise the genomes of rhabdoviruses and paramyxoviruses are among the largest functional RNA molecules and are exceeded in size only by those of the plus-strand coronaviruses. Reanney (1982Reanney ( , 1984 has calculated that the upper size limit for any RNA virus genome cannot be much in excess of 17,600 nucleotides (mol. wt. =5.7 x 10 6 ) as a consequence of the low copying fidelity of RNA polymerases. The segmentation of the genomes of the other negative-strand viruses may be a consequence of such constraints on molecular size or a device for decoupling the transcription of !ndividual genes. Whatever the reason, the genetic properties of the segmented-genome viruses differ substantially from those of the unsegmented-genome viruses, because variation in the former is generated by reassortment of genome subunits as well as by mutation. Mutation is the sole mechanism of variation in unsegmented-genome viruses, since the intramolecular recombination observed with positive-

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Study of the ref(2)P locus of Drosophila melanogaster

Contamine

1993

Molec. Gen. Genet.

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The ref(2)P gene of Drosophila melanogaster is implicated in sigma rhabdovirus multiplication. Two common alleles of ref(2)P are known, ref(2)P0 which permits sigma virus multiplication and ref(2)Pp which is restrictive for most sigma virus strains. This gene maps to the cytogenetic region 37E3-F3. Using Df(2L)E55 (= Df(2L)37D2-E1;37F5-38A1), we have screened for lethal, semi-lethal and visible mutations following diepoxybutane (DEB) or ethyl methanesulfonate (EMS) mutagenesis. Our data confirm than DEB is more efficient than EMS at inducing deletions. The mutations obtained in this region define 14 complementation groups. One of them, l(2)37Dh, appears to be a general enhancer of Minute and Minute-like mutations. None of the mutations were allelic to the ref(2)P locus. Loss-of-function alleles of ref(2)P (called null) were selected following DEB mutagenesis. Homozygous or hemizygous ref(2)Pnull flies are male sterile. These flies, like homozygous or hemizygous ref(2)P0 flies, are fully permissive for sigma virus replication. We suggest that the ref(2)P products interact with viral products, but that this interaction is not necessary for an efficient viral cycle.

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Role of the Drosophila genome in Sigma virus multiplication I. Role of the ret(2)P gene; Selection of host-adapted mutants at the nonpermissive allele Pp

Cited by 20 publications

References 10 publications

Control of Sigma Virus Multiplication by the ref(2)P Gene of Drosophila melanogaster: An in Vivo Study of the PB1 Domain of Ref(2)P

Control of Sigma Virus Multiplication by the ref(2)P Gene of Drosophila melanogaster: An in Vivo Study of the PB1 Domain of Ref(2)P

Rhabdovirus Genetics

Study of the ref(2)P locus of Drosophila melanogaster

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