Role of the dissolved zinc ion and reactive oxygen species in cytotoxicity of ZnO nanoparticles

Song, Wei; Zhang, Jinyang; Guo, Jing; Zhang, Jinhua; Ding, Feng; Li, Liying; Sun, Zengtian

doi:10.1016/j.toxlet.2010.10.003

Cited by 565 publications

(399 citation statements)

References 35 publications

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“…Thus, the almost generic oxidative stress that has been observed with zinc oxide 5,14 , can be attributed to a more general perturbation leading to an imbalance in cellular redox cycles and thus to ROS production.…”

Section: Discussionmentioning

confidence: 99%

Analysis of cellular responses of macrophages to zinc ions and zinc oxide nanoparticles: a combined targeted and proteomic approach

et al. 2014

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Two different zinc oxide nanoparticles, as well as zinc ion, are used to study the cellular responses of the RAW264 macrophage cell line. A proteomic screen is used to provide a wide view of the molecular effects of zinc, and the most prominent results are crossvalidated by targeted studies. Furthermore, the alteration of important macrophage functions (e.g. phagocytosis) by zinc is also investigated. The intracellular dissolution/uptake of zinc is also studied to further characterize zinc toxicity. Zinc oxide nanoparticles dissolve readily in the cells, leading to high intracellular zinc concentrations, mostly as protein-bound zinc. The proteomic screen reveals a rather weak response in the oxidative stress response pathway, but a strong response both in the central metabolism and in the proteasomal protein degradation pathway. Targeted experiments confirm that carbohydrate catabolism and proteasome are critical determinants of sensitivity to zinc, which also induces DNA damage. Conversely, glutathione levels and phagocytosis appear unaffected at moderately toxic zinc concentrations.3

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Section: Discussionmentioning

confidence: 99%

Analysis of cellular responses of macrophages to zinc ions and zinc oxide nanoparticles: a combined targeted and proteomic approach

et al. 2014

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“…Previous studies described different methods to prepare NP suspension, either directly in the medium by simple agitation (Yang et al 2009;Yuan et al 2010), by using bath sonication (Huang et al 2010;Pujalté et al 2011;Song et al 2010;Xiong et al 2011) or stabilisers such as bovine serum albumin (Tantra et al 2010;Yang et al 2009) to eliminate possible aggregates. However, it was recently recommended to not discard these aggregates since their implication in the toxicity of ZnO NPs in embryo and adult zebrafish (Bai et al 2010;Xiong et al 2011) and human cells (Yuan et al 2010) was demonstrated.…”

Section: Discussionmentioning

confidence: 99%

“…ZnO NPs have been shown to produce in vitro cytotoxic, genotoxic, inflammatory and oxidative stress responses in different mammalian cells (Xia et al 2008;Yang et al 2009;Huang et al 2010;Song et al 2010;De Berardis et al 2010;Yuan et al 2010;Hsiao & Huang 2011;Pujalté et al 2011). Studies in mammalian cell lines also attributed the toxicity of ZnO NPs, at least in part, to their solubility, the latter leading to Zn ion formations (Xia et al 2008;De Berardis et al 2010;Song et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Studies in mammalian cell lines also attributed the toxicity of ZnO NPs, at least in part, to their solubility, the latter leading to Zn ion formations (Xia et al 2008;De Berardis et al 2010;Song et al 2010). It was also proposed that the production of reactive oxygen species (ROS) is the main factor responsible for the observed cytotoxicity (Xia et al 2008;Sharma et al 2009;De Berardis et al 2010;Huang et al 2010;Song et al 2010). The contribution of the size and shape to the mammalian cytotoxicity of different ZnO NPs has also been reported, but different conclusions have been reached (Lin et al 2009;Deng et al 2009;Hsiao & Huang 2011).…”

Section: Introductionmentioning

confidence: 99%

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Comparative cytotoxicity induced by bulk and nanoparticulated ZnO in the fish and human hepatoma cell lines PLHC-1 and Hep G2

Fernández‐Cruz¹,

Lammel²,

Connolly³

et al. 2012

Nanotoxicology

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Navas (2013) Comparative cytotoxicity induced by bulk and nanoparticulated ZnO in the fish and human hepatoma cell lines PLHC-1 and Hep G2, Nanotoxicology, 7:5, 935-952, DOI: 10.3109/17435390.2012 AbstractThe increasing presence of ZnO nanoparticles (NPs) in consumer products may be having a dramatic impact in aquatic environments. The evaluation of ZnO NP toxicity represents a great challenge. This study aimed at evaluating the cytotoxic effect of micro-and nanosized ZnO in a fish and a mammalian hepatoma cell line. A detailed characterisation of the particles in exposure media showed that ZnO NPs formed large aggregates. ZnO cytotoxicity was evaluated with a battery of in vitro assays including LUCS, a new approach based on DNA alteration measurements. In fish cells, ZnO NP aggregates contributed substantially to the cytotoxic effects whereas toxicity in the human cells appeared to be mainly produced by the dissolved fraction. ROS production did not contribute to the observed cytotoxicity. This work also showed that measuring concentrations of NPs is essential to understand the mechanisms underlying their toxicity.

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“…Quando em excesso ele parece estar associado com uma exacerbação da resposta inflamatória (SONG et al, 2010;TSOU et al, 2010), redução da absorção do Cu, declive no teor sérico de HDL (BARCELOUX, 1999) e disfunção da função renal e hepática (MIRAZI et al, 2012). …”

Section: Zinco (Zn)unclassified

Efeito da sazonalidade na exposição ao mercúrio e nos marcadores do estresse oxidativo e de inflamação em populações ribeirinhas da Amazônia

Valentini¹

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Role of the dissolved zinc ion and reactive oxygen species in cytotoxicity of ZnO nanoparticles

Cited by 565 publications

References 35 publications

Analysis of cellular responses of macrophages to zinc ions and zinc oxide nanoparticles: a combined targeted and proteomic approach

Analysis of cellular responses of macrophages to zinc ions and zinc oxide nanoparticles: a combined targeted and proteomic approach

Comparative cytotoxicity induced by bulk and nanoparticulated ZnO in the fish and human hepatoma cell lines PLHC-1 and Hep G2

Efeito da sazonalidade na exposição ao mercúrio e nos marcadores do estresse oxidativo e de inflamação em populações ribeirinhas da Amazônia

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