Role of the cofilin 2 gene in regulating the myosin heavy chain genes in mouse myoblast C2C12 cells

Zhu, Haibo; Yang, Huixin; Zhao, Song; Zhang, Junfeng; Liu, Dan; Tian, Yuan; Zhi-yi, Shen; Su, Yuhong

doi:10.3892/ijmm.2017.3272

Cited by 5 publications

(6 citation statements)

References 28 publications

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“…CFL2 localized between Z-discs in myofibrils, which affected cell proliferation, apoptosis, migration, and invasion by regulating actin filaments [51]. In addition, the expression of four MyHC isoforms in undifferentiated myoblast cells with CFL2 RNAi was found to be significantly decreased compared with that in differentiated myoblast cells [52]. Our result indicated that the down-regulated expression of miR-192 may be involved in the regulation of MyHC via the up-regulated expression of CFL2.…”

Section: Discussionmentioning

confidence: 67%

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Jia

Liu

et al. 2020

Genes

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Studies of the gene and miRNA expression profiles associated with the postnatal late growth, development, and aging of skeletal muscle are lacking in sika deer. To understand the molecular mechanisms of the growth and development of sika deer skeletal muscle, we used de novo RNA sequencing (RNA-seq) and microRNA sequencing (miRNA-seq) analyses to determine the differentially expressed (DE) unigenes and miRNAs from skeletal muscle tissues at 1, 3, 5, and 10 years in sika deer. A total of 51,716 unigenes, 171 known miRNAs, and 60 novel miRNAs were identified based on four mRNA and small RNA libraries. A total of 2,044 unigenes and 11 miRNAs were differentially expressed between adolescence and juvenile sika deer, 1,946 unigenes and 4 miRNAs were differentially expressed between adult and adolescent sika deer, and 2,209 unigenes and 1 miRNAs were differentially expressed between aged and adult sika deer. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that DE unigenes and miRNA were mainly related to energy and substance metabolism, processes that are closely associate with the growth, development, and aging of skeletal muscle. We also constructed mRNA–mRNA and miRNA–mRNA interaction networks related to the growth, development, and aging of skeletal muscle. The results show that mRNA (Myh1, Myh2, Myh7, ACTN3, etc.) and miRNAs (miR-133a, miR-133c, miR-192, miR-151-3p, etc.) may play important roles in muscle growth and development, and mRNA (WWP1, DEK, UCP3, FUS, etc.) and miRNAs (miR-17-5p, miR-378b, miR-199a-5p, miR-7, etc.) may have key roles in muscle aging. In this study, we determined the dynamic miRNA and unigenes transcriptome in muscle tissue for the first time in sika deer. The age-dependent miRNAs and unigenes identified will offer insights into the molecular mechanism underlying muscle development, growth, and maintenance and will also provide valuable information for sika deer genetic breeding.

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Section: Discussionmentioning

confidence: 67%

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Jia

Liu

et al. 2020

Genes

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“…This fact may be related to the myogenic regulatory roles of the CFL2 gene in myoblasts; therefore, we intend to develop a new system for myogenic differentiation monitored by CFL2 from the perspective of molecular genetics and epigenetics. CFL2 depletion inactivated cell differentiation progression and inhibited the expressions of myogenic transcription factors, thereby eventually leading to impaired myogenic differentiation in myoblasts [ 11 , 42 ]. In light of this, we observed that decreased expression of CFL2 significantly suppressed myoblast differentiation.…”

Section: Discussionmentioning

confidence: 99%

New Insight into Muscle-Type Cofilin (CFL2) as an Essential Mediator in Promoting Myogenic Differentiation in Cattle

Sun

Zhao

et al. 2022

Bioengineering

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Meat quality and meat composition are not separated from the influences of animal genetic improvement systems; the growth and development of skeletal muscle are the primary factors in agricultural meat production and meat quality. Though the muscle-type cofilin (CFL2) gene has a crucial influence on skeletal muscle fibers and other related functions, the epigenetic modification mechanism of the CFL2 gene regulating meat quality remains elusive. After exploring the spatiotemporal expression data of CFL2 gene in a group of samples from fetal bovine, calf, and adult cattle, we found that the level of CFL2 gene in muscle tissues increased obviously with cattle age, whereas DNA methylation levels of CFL2 gene in muscle tissues decreased significantly along with cattle age by BSP and COBRA, although DNA methylation levels and mRNA expression levels basically showed an opposite trend. In cell experiments, we found that bta-miR-183 could suppress primary bovine myoblast differentiation by negatively regulated CFL2. In addition, we packaged recombinant adenovirus vectors for CFL2 gene knockout and overexpression and found that the CFL2 gene could promote the differentiation of primary bovine myoblasts by regulating marker genes MYOD, MYOG and MYH3. Therefore, CFL2 is an essential mediator for promoting myogenic differentiation by regulating myogenic marker genes in cattle myoblasts.

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“…The remodeling of the actin cytoskeleton is an essential step for efficient myoblast fusion (37) and for AchR clustering at the NMJ (52,53). While vinculin, filamin-C, cofilin-2, and myotilin have been associated with myoblast fusion and differentiation (54)(55)(56)(57) and NMJ (58,59), little is known about the role of ACTN1 in muscles.…”

Section: Discussionmentioning

confidence: 99%