Role of the Cell Wall Integrity and Filamentous Growth Mitogen-Activated Protein Kinase Pathways in Cell Wall Remodeling during Filamentous Growth

Birkaya, Barbara; Maddi, Abhiram; Joshi, Jyoti; Free, Stephen J.; Cullen, Paul J.

doi:10.1128/ec.00006-09

Cited by 49 publications

(49 citation statements)

References 175 publications

(130 reference statements)

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“…Thus, WSC1, WSC2 and MID2 were found in screens for highcopy suppressors of mutants defective in the heatshock response (hsf1 [20]). The same three sensors were also found in a screen for genes affecting filamentous growth in S. cerevisiae, a pathway working in concert with CWI signalling but opposite to the high osmolarity glycerol (HOG) pathway [3]. The latter crosstalk was to be expected, since CWI signalling is induced under conditions of low-medium osmolarity, in contrast to the HOG pathway, which, for instance, is induced under high-salt conditions [18].…”

Section: Sensor Distribution and Dynamicsmentioning

confidence: 90%

“…Multicopy suppressor of the colony formation defect of a sly1 ts mutant defective in vesicular trafficking; probably caused by an indirect effect [27] Multicopy suppressor of the cell integrity defect associated with the mitotic arrest of spc110 mutants, which are defective in a component of the spindle pole body; depends on Pkc1 but not on the downstream MAPK module [49] Mid2 is involved in the filamentous growth (FG) response, also requiring the mucin Msb2; mediated by Slt2/Mpk1 [3] Mid2 signalling is involved in the unfolded protein response (UPR) and CWI signals to regulate UPR components through Swi6 [46] Mid2 signalling is involved in the response to acidic stress; mediated through Bck1 and Mpk1/Slt2; Mid2 and Rgd1 (a Rho3/Rho4 GTPase activating protein) independently mediate acidic stress signalling and converge on CWI activation [4] Multicopy MID2 suppresses the cell viability defect of an rgd1 mutant, encoding a Rho-GTPase which regulates Rho3/Rho4…”

Section: Discussionmentioning

confidence: 99%

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Together we are strong—cell wall integrity sensors in yeasts

Rodicio

Heinisch

2010

Yeast

107

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The integrity of the fungal cell wall is ensured by a signal transduction pathway, the so-called CWI pathway, which has best been studied in the model yeast Saccharomyces cerevisiae. In this context, environmental stress and other perturbations at the cell surface are detected by a small set of plasma membrane-spanning sensors, viz. Wsc1, Wsc2, Wsc3, Mid2 and Mtl1. This review covers the recent advances in sensor structure, sensor mechanics, their cellular distribution and their in vivo functions, obtained from genetic, biochemical, cell biological and biophysical investigations.

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Section: Sensor Distribution and Dynamicsmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Together we are strong—cell wall integrity sensors in yeasts

Rodicio

Heinisch

2010

Yeast

107

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“…Yet another approach has been to treat the cell wall with trypsin, and to use the released tryptic peptides for proteomic analyses. Recently we introduced the use of trifluoromethanesulfonic acid (TFMS), which digests the glycosidic bonds found in the glucan and chitin cell wall components and releases deglycosylated cell wall proteins for proteomic analysis (Birkaya et al, 2009; Bowman et al, 2006; Liu and Free, 2015; Maddi et al, 2009; Maddi and Free, 2010; Maddi et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

A proteomic and genetic analysis of the Neurospora crassa conidia cell wall proteins identifies two glycosyl hydrolases involved in cell wall remodeling

Aldabbous

Notaro

et al. 2016

Fungal Genetics and Biology

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A proteomic analysis of the conidial cell wall identified 35 cell wall proteins. A comparison with the proteome of the vegetative hyphae showed that 16 cell wall proteins were shared, and that these shared cell wall proteins were cell wall biosynthetic proteins or cell wall structural proteins. Deletion mutants for 34 of the genes were analyzed for phenotypes indicative of conidial cell wall defects. Mutants for two cell wall glycosyl hydrolases, the CGL-1 β-1,3-glucanase (NCU07523) and the NAG-1 exochitinase (NCU10852), were found to have a conidial separation phenotype. These two enzymes function in remodeling the cell wall between adjacent conidia to facilitate conidia formation and dissemination. Using promoter::RFP and promoter::GFP constructs, we demonstrated that the promoters for 15 of the conidia-specific cell wall genes, including cgl-1 and nag-1, provided for conidia-specific gene expression or for a significant increase in their expression during conidiation.

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“…Mig1 has been implicated in regulating cell-wall integrity (Krause et al 2008), which is controlled by the protein kinase C (PKC) pathway (Levin 2005). In some settings, the PKC pathway can stimulate the filamentous growth pathway (Yashar et al 1995;Birkaya et al 2009). The activity of the PKC pathway, as measured by phosphorylation of the MAPK Slt2, Slt2P, did not correlate with filamentous growth pathway activity in the snf1D or mig1D mig2D double mutants (data not shown).…”

Section: Snf1 Regulates Starvation-dependent Induction Of the Filamenmentioning

confidence: 99%

The Filamentous Growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 Transcriptional Repressors in Saccharomyces cerevisiae

Karunanithi

Cullen

2012

Genetics

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In the budding yeast S. cerevisiae, nutrient limitation induces a MAPK pathway that regulates filamentous growth and biofilm/mat formation. How nutrient levels feed into the regulation of the filamentous growth pathway is not entirely clear. We characterized a newly identified MAPK regulatory protein of the filamentous growth pathway, Opy2. A two-hybrid screen with the cytosolic domain of Opy2 uncovered new interacting partners including a transcriptional repressor that functions in the AMPK pathway, Mig1, and its close functional homolog, Mig2. Mig1 and Mig2 coregulated the filamentous growth pathway in response to glucose limitation, as did the AMP kinase Snf1. In addition to associating with Opy2, Mig1 and Mig2 interacted with other regulators of the filamentous growth pathway including the cytosolic domain of the signaling mucin Msb2, the MAP kinase kinase Ste7, and the MAP kinase Kss1. As for Opy2, Mig1 overproduction dampened the pheromone response pathway, which implicates Mig1 and Opy2 as potential regulators of pathway specificity. Taken together, our findings provide the first regulatory link in yeast between components of the AMPK pathway and a MAPK pathway that controls cellular differentiation.

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Role of the Cell Wall Integrity and Filamentous Growth Mitogen-Activated Protein Kinase Pathways in Cell Wall Remodeling during Filamentous Growth

Cited by 49 publications

References 175 publications

Together we are strong—cell wall integrity sensors in yeasts

Together we are strong—cell wall integrity sensors in yeasts

A proteomic and genetic analysis of the Neurospora crassa conidia cell wall proteins identifies two glycosyl hydrolases involved in cell wall remodeling

The Filamentous Growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 Transcriptional Repressors in Saccharomyces cerevisiae

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