Role of the CD1a molecule in the superantigen-induced activation of MHC class II negative human thymocytes

Gregory, Stéphane; Zilber, Marie-Thérèse; Choqueux, Christine; Mooney, Nuala; Charron, Dominique; Gélin, Catherine

doi:10.1016/s0198-8859(00)00107-5

Cited by 7 publications

(5 citation statements)

References 36 publications

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“…The CD1 family is composed of five glycosylated proteins showing limited polymorphism (3). The CD1a, CD1b, and CD1c isoforms, are included in the CD1 group 1 based on their sequence homology (4), are detected on activated human monocytes (5,6), and are highly expressed on monocyte-derived immature dendritic cells (iDCs) (3) and on dermal dendritic calls (DCs) in vivo.…”

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confidence: 99%

Regulation of CD1a Surface Expression and Antigen Presentation by Invariant Chain and Lipid Rafts

Sloma

Zilber

Vasselon

et al. 2008

The Journal of Immunology

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In immature dendritic cells (DCs), CD1a is almost exclusively expressed at the cell surface and its membrane organization is poorly understood. In this study, we report that MHC class II, invariant chain (Ii), and CD9 molecules are coimmunoprecipitated with CD1a in immature DCs, and that CD1a/Ii colocalization is dependent on lipid raft integrity. In HeLa-CIITA cells CD1a expression leads to increased Ii trafficking to the cell surface, confirming the relevance of this association. Furthermore, silencing of Ii in DCs induces significant CD1a accumulation on the plasma membrane whereas the total CD1a expression remains similar to that of control cells. These data suggest that CD1a recycling is facilitated by the association with the Ii. The CD1a localization in lipid rafts has functional relevance as demonstrated by inhibition of CD1a-restricted presentation following raft disruption. Overall, these findings identify Ii and lipid rafts as key regulators of CD1a organization on the surface of immature DCs and of its immunological function as Ag-presenting molecule.

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mentioning

confidence: 99%

Regulation of CD1a Surface Expression and Antigen Presentation by Invariant Chain and Lipid Rafts

Sloma

Zilber

Vasselon

et al. 2008

The Journal of Immunology

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“…The need of monocyte to T cell contact has also been established by separating the cells by a semi-permeable membrane which allows the diffusion of macromolecules [ 50 ]. Novel pathway of monocyte dependent regulation of T cells has been reported and the relationship between monocytes and T cells is complicated because the molecules expressed on monocytes can be stimulatory, such as CD1a [ 51 ], or inhibitory, such as CD98 [ 52 ]. In this study, purified autologous CD14+ monocytes added to purifed CD3+ T cells promoted the PHA-induced T cell maturation and further increased the percentage of CD45RA-CD45RO+ cells in Day 6 culture.…”

Section: Discussionmentioning

confidence: 99%

Insulin-like growth factor I promotes cord blood T cell maturation through monocytes and inhibits their apoptosis in part through interleukin-6

Law

Tu²,

Liu

et al. 2008

BMC Immunol

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Background: The functional immaturity of T cells contributes to the susceptibility of neonates to infections and the less severe graft-versus-host disease associated with cord blood (CB) transplantation. We have previously reported that insulin-like growth factor -I (IGF-I) promotes the phytohaemagglutinin (PHA)-induced CB T cell maturation and inhibits their apoptosis in mononuclear cell (MC) culture. We hypothesized that the effects of IGF-I may be mediated by accessory cells and soluble factors.

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“…For example, SEB or SEB-derived peptides could be presented in the context of CD1 instead of the conventional MHC class II molecules, as suggested for other superantigens. 34 In addition, one may not rule out that the expression of The titre for each sample was expressed as the reciprocal of the highest dilution that presented an absorbance value higher than the cut-off value. The columns represent the geometric means of serum titres (reciprocal of serum dilution) of six to eight mice for each experimental group, and the vertical bars represent the standard errors of the means (n ¼ 4 mice/group).…”

Section: Discussionmentioning

confidence: 99%

“…For example, SEB or SEB-derived peptides could be presented in the context of CD1 instead of the conventional MHC class II molecules, as suggested for other superantigens. 34 In addition, one may not rule out that the expression of Va14-Ja281 by most of the NK1.1 + T cells diminishes the overall affinity of the Vb8 chain for the SEB-MHC class II complex. Such experimental evidence has already been provided in other models, indicating that the Va chain might also be of importance in such an interaction.…”

Section: Discussionmentioning

confidence: 99%

Natural killer T cells are required for the development of a superantigen‐driven T helper type 2 immune response in mice

et al. 2005

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SummaryWe show, here, that one single injection or weekly injections of staphylococcal enterotoxin B (SEB), starting in 1-day-old newborn mice, induced a powerful immune response with a T helper type 2 (Th2) pattern, as judged by the isotype and cytokine profile, with the production of large amounts of SEB-specific immunoglobulin G1 (IgG1), detectable levels of SEB-specific IgE and increased production of interleukin-4 by spleen cells. These protocols also induced an increase in the levels of total IgE in the serum. Memory of SEB was transferred to secondary recipients by using total spleen cells from primed animals. The secondary humoral response in transferred mice was diminished if spleen cells from SEB-treated mice were previously depleted of CD3 + or Vb8 + T cells or NK1.1 + cells. In vivo depletion of NK1.1 + cells in adult mice resulted in a marked reduction in the SEB-specific antibody response in both the primary and secondary immune responses. Additionally, purified NK1.1 + T cells were able to perform SEB-specific helper B-cell actions in vitro and in vivo. These results suggest that NK1.1 + T cells are required for the full development of humoral immunological memory, whilst making neonatal tolerance to SEB unachievable.

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Role of the CD1a molecule in the superantigen-induced activation of MHC class II negative human thymocytes

Cited by 7 publications

References 36 publications

Regulation of CD1a Surface Expression and Antigen Presentation by Invariant Chain and Lipid Rafts

Regulation of CD1a Surface Expression and Antigen Presentation by Invariant Chain and Lipid Rafts

Insulin-like growth factor I promotes cord blood T cell maturation through monocytes and inhibits their apoptosis in part through interleukin-6

Natural killer T cells are required for the development of a superantigen‐driven T helper type 2 immune response in mice

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