Role of the Botrytis cinerea FKBP12 ortholog in pathogenic development and in sulfur regulation

Meléndez, Heber Gamboa; Billon-Grand, Geneviève; Fèvre, Michel; Mey, Géraldine

doi:10.1016/j.fgb.2008.11.011

Cited by 32 publications

(32 citation statements)

References 91 publications

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“…Our previous study also indicated that tomato FKBP12 could restore the inhibitory effect of rapamycin to TOR (Xiong et al , ). Likewise, in B. cinerea , FKBP12 homologous protein was found with conserved function in bridging rapamycin and FK506, and it was also reported that BcFKBP12 is involved in T4 strain virulence and sulphur regulation (Gioti et al , ; Melendez et al , ). Here, to further confirm BcFKBP12 function in TOR signalling, we cloned and expressed BcFKBP12 in Arabidopsis .…”

Section: Resultsmentioning

confidence: 95%

“…has been reported. TOR signalling was particularly well characterized in F. graminearum (Bastidas et al, 2012;Lopez-Berges et al, 2010;Melendez et al, 2009;Teichert et al, 2006;Yu et al, 2014), suggesting that a conserved TOR pathway also exists in plant pathogenic fungi.…”

Section: Discussionmentioning

confidence: 99%

“…Rapamycin was discovered in the early 1970s as an antifungal agent against the pathogenic yeast Candida albicans (Sehgal et al ., ). Subsequent studies also found that rapamycin is effective against many phytopathogenic fungi, such as Botrytis cinerea , Mucor circinelloides , Fusarium fujikuroi , Fusarium oxysporum and Fusarium graminearum (Bastidas et al , ; Lopez‐Berges et al , ; Melendez et al , ; Teichert et al , ; Yu et al , ), indicating that a conserved TOR pathway also exists in plant pathogenic fungi. In addition, the research on TOR signalling in F. graminearum elucidated the TOR components and conserved functions in growth and development as well as its role in virulence (Yu et al , ).…”

Section: Introductionmentioning

confidence: 98%

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Host‐induced gene silencing of BcTOR in Botrytis cinerea enhances plant resistance to grey mould

Xiong

Liu

Zhuo

et al. 2019

Molecular Plant Pathology

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Summary Botrytis cinerea is the causal agent of grey mould for more than 200 plant species, including economically important vegetables, fruits and crops, which leads to economic losses worldwide. Target of rapamycin (TOR) acts a master regulator to control cell growth and proliferation by integrating nutrient, energy and growth factors in eukaryotic species, but little is known about whether TOR can function as a practicable target in the control of plant fungal pathogens. Here, we characterize TOR signalling of B. cinerea in the regulation of growth and pathogenicity as well as its potential value in genetic engineering for crop protection by bioinformatics analysis, pharmacological assays, biochemistry and genetics approaches. The results show that conserved TOR signalling occurs, and a functional FK506‐binding protein 12 kD (FKBP12) mediates the interaction between rapamycin and B. cinerea TOR (BcTOR). RNA sequencing (RNA‐Seq) analysis revealed that BcTOR displayed conserved functions, particularly in controlling growth and metabolism. Furthermore, pathogenicity assay showed that BcTOR inhibition efficiently reduces the infection of B. cinerea in plant leaves of Arabidopsis and potato or tomato fruits. Additionally, transgenic plants expressing double‐stranded RNA of BcTOR through the host‐induced gene silencing method could produce abundant small RNAs targeting BcTOR, and significantly block the occurrence of grey mould in potato and tomato. Taken together, our results suggest that BcTOR is an efficient target for genetic engineering in control of grey mould, and also a potential and promising target applied in the biocontrol of plant fungal pathogens.

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Section: Resultsmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Host‐induced gene silencing of BcTOR in Botrytis cinerea enhances plant resistance to grey mould

Xiong

Liu

Zhuo

et al. 2019

Molecular Plant Pathology

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“…S3). Resistance of FKBP12 deletion mutants to rapamycin has been documented in several other fungi, including A. nidulans , B. cinerea , Candida albicans , Cryptococcus neoformans , Fusarium fujikuroi and Mucor circinelloides (Cruz et al ., , ; Fitzgibbon et al ., ; Teichert et al ., ; Melendez et al ., ; Bastidas et al ., ). In addition, rapamycin affects sexual development rather than vegetative growth, and this development is mediated by Fkbp12 via a mechanism independent of the TOR pathway, as shown in fission yeast (Weisman et al ., ).…”

Section: Discussionmentioning

confidence: 98%

The TOR signaling pathway regulates vegetative development and virulence in Fusarium graminearum

et al. 2014

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Summary The target of rapamycin (TOR) signaling pathway plays critical roles in controlling cell growth in a variety of eukaryotes. However, the contribution of this pathway in regulating virulence of plant pathogenic fungi is unknown. We identified and characterized nine genes encoding components of the TOR pathway in Fusarium graminearum. Biological, genetic and biochemical functions of each component were investigated. The FgFkbp12‐rapamycin complex binds to the FgTor kinase. The type 2A phosphatases FgPp2A, FgSit4 and FgPpg1 were found to interact with FgTap42, a downstream component of FgTor. Among these, we determined that FgPp2A is likely to be essential for F. graminearum survival, and FgSit4 and FgPpg1 play important roles in cell wall integrity by positively regulating the phosphorylation of FgMgv1, a key MAP kinase in the cell wall integrity pathway. In addition, the FgPpg1 interacting protein, FgTip41, is involved in regulating mycelial growth and virulence. Notably, FgTip41 does not interact with FgTap42 but with FgPpg1, suggesting the existence of FgTap42:FgPpg1:FgTip41 heterotrimer in F. graminearum, a complex not observed in the yeast model. Collectively, we defined a genetic regulatory framework that elucidates how the TOR pathway regulates virulence and vegetative development in F. graminearum.

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“…The first genome assemblies of two B. cinerea strains, B05.10 and T4, were sequenced using Sanger technology at low coverage [12,13]. Genome sequences of B. cinerea have played a major role in facilitating genetic manipulations and analyzing the genetic basis of pathogenicity [14]. Moreover, high-coverage de novo assemblies of genome sequences have promoted the development of genome-wide transcriptomic and proteomic techniques in B. cinerea [5].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide Transcriptomic Analysis of the Response ofBotrytis cinereato Wuyiencin

Shi

Liu

Wei

et al. 2019

Preprint

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AbstractGrey mould is caused by the ascomycetes Botrytis cinerea in a range of crop hosts. As a biological control agent, the nucleoside antibiotic wuyiencin has been industrially produced and widely used as an effective fungicide. To elucidate the effects of wuyiencin on the transcriptional regulation in B. cinerea, we, for the first time, report a genome-wide transcriptomic analysis of B. cinerea treated with wuyiencin. We could identify 2067 differentially expressed genes (DEGs); of them, 886 and 1181 genes were significantly upregulated and downregulated, respectively. Functional categorization indicated that genes involved in amino acid metabolism and those encoding putative secreted proteins were remarkably influenced in response to wuyiencin treatment. Moreover, the expression of genes involved in protein synthesis and energy metabolism (oxidative phosphorylation) and of those encoding ATP-binding cassette transporters was markedly upregulated, whereas that of genes participating in DNA replication, cell cycle, and stress response was downregulated. Furthermore, wuyiencin resulted in mycelial malformation and negatively influenced cell growth rate and conidial yield in B. cinerea. Our results suggest that this nucleoside antibiotic regulates all aspects of cell growth and differentiation in B. cinerea. To summarize, we identified candidate pathways and target genes that may offer insights into the protective and antagonistic mechanisms underlying the action of biological control agents.

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Role of the Botrytis cinerea FKBP12 ortholog in pathogenic development and in sulfur regulation

Cited by 32 publications

References 91 publications

Host‐induced gene silencing of BcTOR in Botrytis cinerea enhances plant resistance to grey mould

Host‐induced gene silencing of BcTOR in Botrytis cinerea enhances plant resistance to grey mould

The TOR signaling pathway regulates vegetative development and virulence in Fusarium graminearum

Genome-wide Transcriptomic Analysis of the Response ofBotrytis cinereato Wuyiencin

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