Role of the Ascorbate-Glutathione Antioxidant System in Chilling Resistance of Tomato

Walker, Mark; McKersie, Bryan D.

doi:10.1016/s0176-1617(11)80766-2

Cited by 180 publications

(115 citation statements)

References 17 publications

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“…The reduction in peroxidation and cell viability towards the end of the cold exposure observed in AGB002 may be due to the better antioxidant enzyme machinery. The present data support the contention that cold stress induced generation of ROS may be responsible for cellular damage and induction of antioxidant enzymes (Kendall and Mckersie, 1989;Kuroda and Sagisaka, 1992;Walker and McKersie, 1993;Vyas and Kumar, 2005).…”

Section: Cell Damage and Oxidative Stresssupporting

confidence: 90%

“…Greater increase in GR would certainly help in maintaining a higher GSH/GSSG ratio, which is essential in ensuring the correct folding and appropriate activity of the proteins and redox signaling of various defense genes (Polidoros and Scandalios, 1999). High GR activities have been shown in various chilling tolerant genotypes (Walker and McKersie, 1993;Kocsy et al, 1996). It is quite likely that increased levels of GR keep glutathione in the reduced state and play a significant role in protecting cellular homeostasis against cold stress damage in W. somnifera.…”

Section: Antioxidant Enzymesmentioning

confidence: 99%

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Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Mir

Khazir

et al. 2015

Industrial Crops and Products

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Withania somnifera (L.) Dunal (Indian ginseng) is a high value medicinal plant. It synthesizes a large array of biologically active withanolides. In this study, two month old seedlings of AGB002 (wild genotype) and AGB025 (cultivated genotype) of W. somnifera were subjected to cold stress (4 • C) under controlled envi-ronment. Plants were analyzed for three medicinally important secondary metabolites (withanolide A, withanone and withaferin A), lipid peroxidation (MDA), cell injury, superoxide radical (O 2 •− ) accumula-tion and anti-oxidative enzymes activities such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR). Increases in the titers of superoxide anion and MDA were observed from day 1 to day 7 in both genotypes, although the increase on the first day of exposure was significantly higher. Enzymatic activities of SOD, CAT, APX and GR also showed an increasing trend in both genotypes and reached a maximum on day 7 of the cold temperature exposure; however, this increase was higher in AGB002 than AGB025. Withanolide A (WS-1) in the roots of both genotypes sig-nificantly decreased on the first day of cold exposure and then showed a recovery until day 7. WS-1 was not detected in the leaves of either genotype. Withanone (WS-2) content in the leaves also decreased towards the end of the cold period in both genotypes. Cold stress also elicited the accumulation of WS-2 in AGB025, but was not detectable in control seedlings. At maturity, WS-2 was also detected in control plants. Furthermore, a significant increase in the leaf withaferin A (WS-3) content was recorded from day 1 to day 7 of the cold exposure in both the genotypes, suggesting the possible involvement of with-anolides in cold-protection. AGB002 showed comparatively higher accumulation of antioxidant enzymes and selected marker withanolides than AGB025, indicating that AGB002 is better adapted to cold than AGB025. It could be inferred from these observations that cold stress induces bioactive withanolide accu-mulation in W. somnifera as a mechanism for scavenging reactive oxygen species (ROS). These studies also provide an impetus for enhancing the withanolide accumulation in W. somnifera using controlled environment technology.

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Section: Cell Damage and Oxidative Stresssupporting

confidence: 90%

Section: Antioxidant Enzymesmentioning

confidence: 99%

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Mir

Khazir

et al. 2015

Industrial Crops and Products

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“…Increases in ROS generation and resultant peroxidation of membrane lipids in chilled leaves are common to chilling-sensitive species and cultivars. Chilling tolerant plants are likely to have more efficient antioxidant systems than chilling sensitive ones (Walker & McKersie 1993). However, the mechanism of chill-induced ROS generation has yet to be deciphered in detail.…”

Section: Chillingmentioning

confidence: 99%

Polyamines: potent modulators of plant responses to stress

Fariduddin

Varshney

Yusuf

et al. 2013

Journal of Plant Interactions

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Polyamines (PAs) are aliphatic polycations that are widespread in living organisms. In this review, we are focusing the correlation between endogenous PA titers and physiological perturbations and on the protective role of various analogues of PAs against abiotic stresses. PAs are involved in many physiological processes, such as cell growth and development and also respond to diverse abiotic stresses. Polyamines level shifts in different ways depending on several factors, such as plant species, tolerance or sensitivity to stress, and duration of stress. Exogenously supplied PAs protected plants from abiotic stress, whereas transgenic plants overexpressing PA biosynthetic genes exhibited stress tolerance. On the other hand, loss-of-function mutant of PA biosynthetic genes, or decrease of PA titers, resulted to decrease stress tolerance.

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“…Some data relate the loss of the germination capability to the drop in the antioxidant system efficiency (Tommasi et al 2006;Varghese J. Qinand Naithani (2007)). Several studies have demonstrated that freezing tolerance in various species is partly the result of an enhanced antioxidant defense system (Kendall and McKersie 1989;Walker and McKersie 1993;Prasad 1996Prasad , 1997Hodges et al 1997). However, research about the effects of seasonal frozen soil treatment on seeds and the related antioxidant systems is scarce.…”

Section: Introductionmentioning

confidence: 99%

Oxidative metabolism‐related changes during germination of mono maple (Acer mono Maxim.) seeds under seasonal frozen soil

Qin

Liu

2009

Ecological Research

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Oxidative metabolism-related changes during germination of mono maple (Acer mono Maxim.) seeds under seasonal frozen soil Abstract The influence of seasonal frozen soil and buried depth on germination of mono maple (Acer mono Maxim.) seeds was studied in field conditions in winter in a sub-alpine region. Mono maple seeds almost lost their ability to germinate in non-freezing soil, while seasonal frozen soil treatments facilitated the germination accompanied with a progressive accumulation of reactive oxygen species (ROS). The result indicates that ROS may act as a positive signal for seed dormancy. However, exceeding accumulation of ROS led to decrease in germination rate. We suggest that the shift from a signaling to a deleterious role may be related to the accumulation of these ROS above a threshold level that leads to various cellular alterations and damage. The enhanced activities of antioxidant enzymes appear to be more closely related to freezing tolerance, because of their ability to scavenge ROS to avoid deleterious events. Seasonal frozen soil was beneficial in accelerating the germination of mono maple seeds. However, a slight increase of freezing temperature may have also facilitated the germination of mono maple seeds by enhanced activities of antioxidant enzymes. Hence, moderate winter warming may be beneficial to mono maple regeneration due to the improvement of seed germination, but the disappearance of seasonal frozen soil may lead to germination failure of the mono maple seeds.

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Role of the Ascorbate-Glutathione Antioxidant System in Chilling Resistance of Tomato

Cited by 180 publications

References 17 publications

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Polyamines: potent modulators of plant responses to stress

Oxidative metabolism‐related changes during germination of mono maple (Acer mono Maxim.) seeds under seasonal frozen soil

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