Role of the antioxidant defense system during the production of lignocellulolytic enzymes by fungi

Pompeu, Geórgia Bertoni; Pietrobon, Vivian Cristina; Andreote, Cristiane Cipolla Fasanella; Ferreira, Luiz Fernando Romanholo; Aguiar, Mario; Sartori, Sérgio Birello; Cruz, Sandra Helena da; Monteiro, Regina Teresa Rosim

doi:10.1007/s10123-018-00045-1

Cited by 13 publications

(7 citation statements)

References 52 publications

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“…Involvement of laccases in the defense against oxidative stress has been demonstrated in several fungi, as well as bacteria, as ROS scavengers and/or by activation of other antioxidative systems (Jaszek et al ., 2006 a, b; Santhanam et al ., 2011; Strong and Claus, 2011; Yang et al ., 2012; Pompeu et al ., 2019). Here, we found the BbLac2 was specifically expressed in B. bassiana when proliferated in the insect haemocoel, and induced by oxidative stress, which was in line with increased or decreased resistance to oxidative stress for BbLac2 OE or Δ BbLac2 strains, respectively.…”

Section: Discussionmentioning

confidence: 99%

Multifunctional role of a fungal pathogen‐secreted laccase 2 in evasion of insect immune defense

Deng

Wang

et al. 2021

Environmental Microbiology

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Summary Laccases are widely present in bacteria, fungi, plants and invertebrates and involved in a variety of physiological functions. Here, we report that Beauveria bassiana, an economic important entomopathogenic fungus, secretes a laccase 2 (BbLac2) during infection that detoxifies insect immune response‐generated reactive oxygen species (ROS) and interferes with host immune phenoloxidase (PO) activation. BbLac2 is expressed in fungal cells during proliferation in the insect haemocoel and can be found to distribute on the surface of haemolymph‐derived in vivo fungal hyphal bodies or be secreted. Targeted gene‐knockout of BbLac2 increased fungal sensitivity to oxidative stress, decreased virulence to insect, and increased host PO activity. Strains overexpressing BbLac2 showed increased virulence, with reduced host PO activity and lowered ROS levels in infected insects. In vitro assays revealed that BbLac2 could eliminate ROS and oxidize PO substrates (phenols), verifying the enzymatic functioning of the protein in detoxification of cytotoxic ROS and interference with the PO cascade. Moreover, BbLac2 acted as a cell surface protein that masked pathogen associated molecular patterns (PAMPs), enabling the pathogen to evade immune recognition. Our data suggest a multifunctional role for fungal pathogen‐secreted laccase 2 in evasion of insect immune defenses.

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Section: Discussionmentioning

confidence: 99%

Multifunctional role of a fungal pathogen‐secreted laccase 2 in evasion of insect immune defense

Deng

Wang

et al. 2021

Environmental Microbiology

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“…Plants then alleviate the damaging effect of elevated ROS level and maintain cellular redox homeostasis in plants by several means including antioxidant enzymes to neutralize free radicals. 89,90 The antioxidant enzymes-based mechanism of ROS scavenging includes CAT, POD and SOD. 91 Glycoproteins impact was not limited to the fungal arial parts but extended to trigger plant defense responses as well.…”

Section: Discussionmentioning

confidence: 99%

Soybean β‐conglycinin and catfish cutaneous mucous p22 glycoproteins deteriorate sporangial cell walls of Pseudoperonospora cubensis and suppress cucumber downy mildew

Atallah

Osman

Ali

et al. 2021

Pest Management Science

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BACKGROUND: Cucumber plants suffer from a serious threatening disease, downy mildew, throughout the growing seasons irrespective of the weather temperature. The causal agent, Pseudoperonospora cubensis, tends to evolve rapidly upon sequential applications of chemical fungicides and generate new progeny possessing tolerance to such fungicides. Glycoproteins represent an environmentally safe alternative for chemically synthetized fungicides and do not trigger fungicide resistance. We studied the antifungal activity of four glycoproteins namely soybean ⊎-conglycinin, chickpea vicilin, duck egg ovomucin and catfish p22 against P. cubensis. Ten commercial fungicides of different chemical groups were used as positive controls of glycoprotein treatments. RESULTS:The results revealed that soybean ⊎-conglycinin and catfish p22 glycoproteins possess significant antifungal activity against P. cubensis. The amount of disease suppression caused by ⊎-conglycinin and p22 was comparable to the highly efficient chemical fungicides containing copper oxychloride, cymoxanil and fosetyl Al as active ingredients. Vicilin and ovomucin were less efficient biocides as they gave moderate suppression of disease severity. However, all tested glycoproteins provided full protection for the newly emerged cucumber leaves. Microscopic examination of glycoprotein-treated leaves inferred the ability of catfish p22 and soybean ⊎-conglycinin to disrupt the integrity of sporangial cell walls of P. cubensis rendering them nonviable compared to untreated ones. Expression levels of total phenolic compounds and the antioxidant enzymes catalase, superoxide dismutase and peroxidase were elevated upon glycoproteins application, which infers their involvement in disease suppression.CONCLUSION: This report emphasizes the direct and indirect roles of glycoproteins in safe management of cucumber downy mildew disease.

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“…Similarly, the tolerance toward extracellular H 2 O 2 may be due to the presence of robust antioxidant enzymes such as catalase, glutathione reductase, superoxide dismutase, and high glutathione content to reduce oxidative stress damage [113]. The transcriptome of the fungus Colletotrichum lupini showed upregulation of catalase and peroxidase genes in response to plant infection [114].…”

Section: Discussionmentioning

confidence: 99%

A Lignocellulolytic Colletotrichum sp. OH with Broad-Spectrum Tolerance to Lignocellulosic Pretreatment Compounds and Derivatives and the Efficiency to Produce Hydrogen Peroxide and 5-Hydroxymethylfurfural Tolerant Cellulases

Chanda

Mozumder

Chorei

et al. 2021

JoF

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Fungal endophytes are an emerging source of novel traits and biomolecules suitable for lignocellulosic biomass treatment. This work documents the toxicity tolerance of Colletotrichum sp. OH toward various lignocellulosic pretreatment-derived inhibitors. The effects of aldehydes (vanillin, p-hydroxybenzaldehyde, furfural, 5-hydroxymethylfurfural; HMF), acids (gallic, formic, levulinic, and p-hydroxybenzoic acid), phenolics (hydroquinone, p-coumaric acid), and two pretreatment chemicals (hydrogen peroxide and ionic liquid), on the mycelium growth, biomass accumulation, and lignocellulolytic enzyme activities, were tested. The reported Colletotrichum sp. OH was naturally tolerant to high concentrations of single inhibitors like HMF (IC50; 17.5 mM), levulinic acid (IC50; 29.7 mM), hydroquinone (IC50; 10.76 mM), and H2O2 (IC50; 50 mM). The lignocellulolytic enzymes displayed a wide range of single and mixed inhibitor tolerance profiles. The enzymes β-glucosidase and endoglucanase showed H2O2- and HMF-dependent activity enhancements. The enzyme β-glucosidase activity was 34% higher in 75 mM and retained 20% activity in 125 mM H2O2. Further, β-glucosidase activity increased to 24 and 32% in the presence of 17.76 and 8.8 mM HMF. This research suggests that the Colletotrichum sp. OH, or its enzymes, can be used to pretreat plant biomass, hydrolyze it, and remove inhibitory by-products.

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Role of the antioxidant defense system during the production of lignocellulolytic enzymes by fungi

Cited by 13 publications

References 52 publications

Multifunctional role of a fungal pathogen‐secreted laccase 2 in evasion of insect immune defense

Multifunctional role of a fungal pathogen‐secreted laccase 2 in evasion of insect immune defense

Soybean β‐conglycinin and catfish cutaneous mucous p22 glycoproteins deteriorate sporangial cell walls of Pseudoperonospora cubensis and suppress cucumber downy mildew

A Lignocellulolytic Colletotrichum sp. OH with Broad-Spectrum Tolerance to Lignocellulosic Pretreatment Compounds and Derivatives and the Efficiency to Produce Hydrogen Peroxide and 5-Hydroxymethylfurfural Tolerant Cellulases

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