Role of the 5′‐terminal sequence in the RNA binding site of yeast 5.8 S rRNA

The nucleotide sequence of the intergenic region between the 5.8S and 26S rRNA genes of the yeast ribosomal RNA operon. Possible implications for the interaction between 5.8S and 26S rRNA and the processing of the primary transcript

“…Homology between the 5'-terminal regions of X. laevis 28S rRNA [17], yeast 26S rRNA and E. coli 23S rRNA [18]. Homologies between yeast and [25][26][27][28]. No information as to the sites on 26S rRNA participating in this association was available until recently, when Kelly and Cox postulated a binding site for 5.8S rRNA near the 3' end of 26S rRNA of Neurospora crassa [29].…”

Nucleotide sequences of Acanthamoeba castellanii 5S and 5.8S ribosomal ribonucleic acids: phylogenetic and comparative structural analyses

“…Helix a can also be criticized on the grounds that the 5'-and 3'-terminal regions of 5.8S rRNA are considered to be involved in specific basepairing with sequences in 26S rRNA [37][38][39][40]. The extensive mis-matching and looping-out of nucleotides required to form helix a in A. castellanii and other 5.8S rRNAs may indicate that this helix is formed only in free molecules in solution [41,42] and has little significance in the ribosome.…”

Sequence analysis of 28S ribosomal DNA from the amphibianXenopus laevis

“…However, unlike the intervening sequence class of insertions, the processing cuts are not followed by a splicing ligation. Perhaps it is not necessary for 5.8S RNA to be covalently joined to 28S rRNA, because both of the 5.8S RNA termini are already hydrogen bonded to 28S rRNA (58,59).…”