Role of TGF- s in normal human endometrium and endometriosis

Omwandho, Charles; Konrad, Lutz; Halis, G; Oehmke, Frank; Tinneberg, Hans‐Rudolf

doi:10.1093/humrep/dep382

Cited by 143 publications

(132 citation statements)

References 92 publications

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“…In all of the women, a specimen of endometrium was collected by hysteroscopy and separated into proliferative (days [5][6][7][8][9][10][11][12][13][14] and secretory phases (day 15 onward), according to the last day of menstruation, transvaginal ultrasound scan, and histologic criteria. In the endometriosis group, all patients showed stage III or IV endometriosis according to the American Society for Reproductive Medicine classification (20).…”

Section: Patients and Tissue Collectionmentioning

confidence: 99%

“…Altered E 2 and P receptor activity and impaired local growth factor and cytokine expression induce proliferation of endometrial cells, peritoneal adhesion, and inflammation of endometriotic lesions (3). Among growth factors, an involvement of transforming growth factor b (TGF-b) superfamily in cell proliferation, immune function, and apoptosis in endometriosis has been shown (4)(5)(6). Antim€ ullerian hormone (AMH), also known as m€ ullerian inhibiting substance (MIS), is a member of TGF-b superfamily (7), playing an essential role in sexual differentiation.…”

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confidence: 99%

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Increased expression of antimüllerian hormone and its receptor in endometriosis

Carrarelli

Rocha

Belmonte

et al. 2014

Fertility and Sterility

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Objective: To evaluate antim€ ullerian hormone (AMH) and AMH receptor II (AMHRII) mRNA and protein expression in endometrium and in ovarian or deep lesions of women with endometriosis. Design: Prospective study. Setting: University hospitals in Italy and Brazil. Patients: Patients with endometriosis (n ¼ 55) and healthy women (n ¼ 45). Interventions: Specimens of endometrium obtained by hysteroscopy from patients with endometriosis and from healthy control subjects; specimens of ovarian endometriosis (n ¼ 29) or of deep endometriosis (n ¼ 26) were collected by laparoscopy. Serum samples were collected in some endometriotic patients (n ¼ 23) and healthy control subjects (n ¼ 20). Main Outcome Measure(s): AMH and AMHRII mRNA levels were evaluated by quantitative reverse-transcription polymerase chain reaction and protein localization by immunohistochemistry. AMH levels in tissue homogenates and in serum were assessed by ELISA. Result(s): Endometrium from women with endometriosis showed higher AMH and AMHRII mRNA levels than control women, with no significant differences between proliferative and secretory phases. Specimens collected from ovarian or deep endometriosis showed the highest AMH and AMHRII mRNA expression. Immunolocalization study confirmed the high AMH and AMHRII protein expression in endometriotic lesions. No difference of serum AMH levels between the groups was found. Conclusion(s):The increased AMH and AMHRII mRNA and protein expression in endometrium and in endometriotic lesions suggests a possible involvement of AMH in endometriosis.

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Section: Patients and Tissue Collectionmentioning

confidence: 99%

mentioning

confidence: 99%

Increased expression of antimüllerian hormone and its receptor in endometriosis

Carrarelli

Rocha

Belmonte

et al. 2014

Fertility and Sterility

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“…Według Omwandho i wsp. (46), transformujące czynniki wzrostu β nie tylko uczestniczą w inicjacji menstruacji u kobiet poprzez skurcz naczyń krwionośnych, ale również w procesach naprawczych tkanek. Co więcej, czynniki te syntetyzowane są przez komórki endometrium oraz makrofagi, przenikając do płynu macicznego mogą oddziaływać na zarodek w okresie okołoimplantacyjnym (24,30,58).…”

Section: Artykuł Przeglądowy Reviewunclassified

“…Co więcej, czynniki te syntetyzowane są przez komórki endometrium oraz makrofagi, przenikając do płynu macicznego mogą oddziaływać na zarodek w okresie okołoimplantacyjnym (24,30,58). Identyfikowane w płynie otrzewnowym kobiet cierpiących na endometriozę, kandydują do miana kluczowych czynników uczestniczących w rozwoju bądź utrzymaniu tej patologii (46). Dla fibroblastów czynniki należące do TGF-β stanowią chemotaksyny, które indukują syntezę kolagenu oraz fibronektyny.…”

Section: Artykuł Przeglądowy Reviewunclassified

Relevant aspects of the influence of chosen growth factors on the activity of the female reproductive system

Woźna¹,

Rybska²,

Swierczewska³

et al. 2017

Medycyna Weterynaryjna

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*) Projekt został sfinansowany ze środków Narodowego Centrum Nauki przyznanych na podstawie decyzji DEC-2013/09/N/NZ5/0183. Woźna M., Rybska M., Świerczewska M., Jaśkowski J. M.Relevant aspects of the influence of chosen growth factors on the activity of the female reproductive system Summary The endometrium of the uterus is a highly dynamic structure in terms of its changes during the various stages of the sexual cycle. These changes are the result of cyclical fluctuations in the concentrations of steroid hormones and local factors of an auto -and paracrine -nature. This condition indicates that the causes of degenerative processes of the uterus must be sought not only in disorders of the hormonal profile and bacterial infections but also disorders at the molecular level. Factors that may play a key role in the formation and development of various pathologies of the female reproductive system include growth factors and their receptors (growth factors -GFs). Discussing these growth factors in the work may provide useful molecular markers that identify pathological conditions of the endometrium. Subject to expression in the endometrium, they are involved in the regulation of cell proliferation, migration and secretion of the glandular epithelium, they also regulate physiological and pathological angiogenesis, revealing strong pro-inflammatory effects. In this research, the authors present an overview of current scientific reports indicating that changes in the expression of studied factors, and thus disturbances in their effects, may constitute one of the causes of pathogenesis within the uterus in many animal species.

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“…These histological events are controlled by ovarian steroids. In addition, many of these processes are considered to be regulated by cytokines and growth factors, several of which are members of the transforming growth factor-β (TGF-β) superfamily (Liu et al, 2004;Omwandho et al, 2010;Bukowska et al, 2011;Tanwar and McFarlane, 2011;Argañaraz et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Expression of bone morphogenetic protein 2, 4, and related components of the BMP signaling pathway in the mouse uterus during the estrous cycle

Yan

Wei

Jin

et al. 2014

J. Zhejiang Univ. Sci. B

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Abstract:The objective was to investigate the expression of bone morphogenetic protein (BMP) family members in the mouse uterus during the estrous cycle by real-time polymerase chain reaction (PCR) and immunohistochemistry. Uterine samples from Swiss ICR mice were collected and dissected free of surrounding tissue. One uterine horn was snap frozen in liquid nitrogen immediately after collection and stored at −80 °C for RNA extraction, and the other was fixed in 40 mg/ml paraformaldehyde at room temperature for immunolocalization of BMP2 protein. Real-time PCR analysis showed that the expression level of Bmp2 was significantly higher at proestrus than at estrus and metestrus (P<0.05). The relative abundance of Bmp4 exhibited significant fluctuations, but there were no statistically significant differences between the expression levels of Bmp2 and Bmp4 (P>0.05). The expression levels of Bmpr1a and Bmpr2 remained unchanged during estrous cycles. However, the level of Bmpr1b mRNA decreased significantly at estrus (P<0.05), increasing subsequently at metestrus. Furthermore, the level of Bmpr1b mRNA was significantly lower than those of Bmpr1a and Bmpr2 mRNA at the corresponding stages (P<0.05). All three receptor-regulated Smads (R-Smads) detected were differentially expressed in the mouse uterus and the expression levels of Smad1 and Smad5 were significantly higher than that of Smad8 (P<0.05). In addition, the expression level of Smad4 did not change substantially throughout the estrous cycle. Immunohistochemical experiments revealed that BMP2 protein was differentially expressed and localized mainly in the uterine luminal and glandular epithelial cells throughout the estrous cycle. In conclusion, our results provide information about the variation in the mRNA levels of Bmp2 and Bmp4 and related components of the BMP signaling pathway. The data provide quantitative and useful information about the roles of endometrial BMP proposed and demonstrated by others, such as the degradation and remodeling of the endometrium.

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Role of TGF- s in normal human endometrium and endometriosis

Cited by 143 publications

References 92 publications

Increased expression of antimüllerian hormone and its receptor in endometriosis

Increased expression of antimüllerian hormone and its receptor in endometriosis

Relevant aspects of the influence of chosen growth factors on the activity of the female reproductive system

Expression of bone morphogenetic protein 2, 4, and related components of the BMP signaling pathway in the mouse uterus during the estrous cycle

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