Role of Store-Operated Calcium Entry During Meiotic Progression and Fertilization of Mammalian Oocytes

Expression of ORAI1, a Plasma Membrane Resident Subunit of the CRAC Channel, in Rodent and Non-rodent Species

“…Of particular importance was the female reproductive system, because we were able to obtain ovaries from young mice, which allowed us to see clear expression in the follicles, consistent with previous observations of the expression of protein in ovum from multiple species (Fig. 6D) (reviewed in (Martín-Romero et al 2012)). In addition, we were able to detect expression in the mouse salivary gland, which had been previously reported (Fig.…”

“…There have been previous reports of ORAI1 expression in mouse, pig, and human ova, and these observations could be confirmed in further-focused studies using appropriate female reproductive tissues (reviewed in (Martín-Romero et al 2012)). …”

Calcium signaling and cell proliferation

“…relation was demonstrated between Ca 2+ oscillation and STIM1 expression and activity, other studies showed a link between cell proliferation/apoptosis, PLC, NF-κB, STIM1, Orai1 and Ca 2+ oscillations [43,[88][89][90][91][92].…”

“…The studies imply that primary function of a large Ca 2+ oscillatory signals might be ensure sufficient store depletion by robust activation of CRAC channels through STIM1, activating downstream signal transduction [43,[88][89][90][91][92]. Additionally, two different transcription factors, c-fos and NFAT were shown to promote local Ca 2+ elevations near open CRAC channels and activate gene expression [42,93].…”

Phospho-STIM1 is a downstream effector that mediates the signaling triggered by IGF-1 in HEK293 cells

“…The participation of STIM1 in cell signaling is a topic under current investigation and recent evidence support for a role in cell adhesion, migration, differentiation, and the control of gene expression [38][39][40][41][42][43][44]. The intense work in this area has focused mainly on the interaction of STIM1 with Ca 2+ channels, and on the phenotypic effect of knocking down STIM1 in cells in vitro.…”