Role of stilbenes in the resistance of grapevine to powdery mildew

Schnée, Sylvain; Viret, Olivier; Gindro, Katia

doi:10.1016/j.pmpp.2008.07.002

Cited by 132 publications

(120 citation statements)

References 21 publications

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“…A vast range of abiotic stress treatments lead to stilbene biosynthesis, including mechanical damage (Chiron et al, 2000;Pezet et al, 2003), exposure to UV-C light (Bais et al, 2000), treatment with chemicals, such as aluminum ions, cyclodextrins, and ozone (Rosemann et al, 1991;Adrian et al, 1996;Zamboni et al, 2009), and the application of plant hormones like ethylene and jasmonates (Belhadj et al, 2008a(Belhadj et al, , 2008bD'Onofrio et al, 2009). In terms of biotic stresses, the synthesis of stilbenes is particularly well documented in grapevine, where it has been shown to be induced upon infection with various pathogens, including Plasmopara viticola (Langcake and Pryce, 1976), Erisyphe necator (Fung et al, 2008;Schnee et al, 2008), and Botrytis cinerea . The biosynthetic pathway leading to the production of stilbenes is a side branch of the general PP pathway and can be considered as an extension of the flavonoid pathway (Vannozzi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera

et al. 2013

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Plant stilbenes are phytoalexins that accumulate in a small number of plant species, including grapevine (Vitis vinifera), in response to biotic and abiotic stresses and have been implicated in many beneficial effects on human health. In particular, resveratrol, the basic unit of all other complex stilbenes, has received widespread attention because of its cardio-protective, anticarcinogenic, and antioxidant properties. Although stilbene synthases (STSs), the key enzymes responsible for resveratrol biosynthesis, have been isolated and characterized from several plant species, the transcriptional regulation underlying stilbene biosynthesis is unknown. Here, we report the identification and functional characterization of two R2R3-MYB-type transcription factors (TFs) from grapevine, which regulate the stilbene biosynthetic pathway. These TFs, designated MYB14 and MYB15, strongly coexpress with STS genes, both in leaf tissues under biotic and abiotic stress and in the skin and seed of healthy developing berries during maturation. In transient gene reporter assays, MYB14 and MYB15 were demonstrated to specifically activate the promoters of STS genes, and the ectopic expression of MYB15 in grapevine hairy roots resulted in increased STS expression and in the accumulation of glycosylated stilbenes in planta. These results demonstrate the involvement of MYB14 and MYB15 in the transcriptional regulation of stilbene biosynthesis in grapevine.

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Section: Introductionmentioning

confidence: 99%

The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera

et al. 2013

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“…Chemical barriers in the form of phenolic antifungal phytoalexins accumulate in and around the infected areas (Schnee et al 2008). The grapevine phenolic compounds are secondary metabolites, of which synthesis involves the coordinated regulation of the phenylpropanoid pathway and its flavonoid (anthocyanin), stilbene (phytoalexins) and lignin branch pathways (Supplementary data Fig.…”

Section: Introductionmentioning

confidence: 99%

Response of Vitis vinifera cell cultures to Eutypa lata and Trichoderma atroviride culture filtrates: expression of defence-related genes and phenotypes

et al. 2016

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Cell suspension cultures of Vitis vinifera cv. Dauphine berries were used to study the response to the vascular pathogen, Eutypa lata, in comparison with a biological control agent, Trichoderma atroviride, that was previously shown to be effective in pruning wound protection. The expression of genes coding for enzymes of the phenylpropanoid pathway and pathogenesis-related (PR) proteins was profiled over a 48-h period using quantitative reverse transcriptase PCR. The cell cultures responded to elicitors of both fungi with a hypersensitive-like response that lead to a decrease in cell viability. Similar genes were triggered by both the pathogen and biocontrol agent, but the timing patterns and magnitude of expression was dependent on the specific fungal elicitor. Culture filtrates of both fungi caused upregulation of phenylalanine ammonia-lyase (PAL), 4-coumaroyl Co-A ligase (CCo-A) and stilbene synthase (STS), and a downregulation of chalcone synthase (CHS) genes. The pathogen filtrate caused a biphasic pattern in the upregulation of PAL and STS genes which was not observed in cells treated with filtrates of the biocontrol agent. Analytical assays showed significantly higher total phenolic content and chitinolytic enzyme activity in the cell cultures treated with the T. atroviride filtrate compared to the pathogen filtrate. These results corresponded well to the higher expression of PAL and chitinase class IV genes. The response of the cell cultures to T. atroviride filtrate provides support for the notion that the wound protection by the biocontrol agent at least partially relies on the induction of grapevine resistance mechanisms.

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“…MSI allowed the identification and mapping of resveratrol and pterostilbene but the biosynthesis of some other stilbenes is also expected to be induced in grapevine leaves and even berries in response to pathogen infection or UV irradiation [19][20][21]. Besides the glycosylation of stilbenes (e.g., piceids), oxidative oligomerization of resveratrol catalyzed by plant peroxidases may occur and several of them have been identified in stressed grapevine leaves [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves

et al. 2014

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Abstract:To investigate the in-situ response to a stress, grapevine leaves have been subjected to mass spectrometry imaging (MSI) experiments. The Matrix Assisted Laser Desorption/Ionisation (MALDI) approach using different matrices has been evaluated. Among all the tested matrices, the 2,5-dihydroxybenzoic acid (DHB) was found to be the most efficient matrix allowing a broader range of detected stilbene phytoalexins. Resveratrol, but also more toxic compounds against fungi such as pterostilbene and viniferins, were identified and mapped. Their spatial distributions on grapevine leaves irradiated by UV show their specific colocation around the veins. Moreover, MALDI MSI reveals that resveratrol (and piceids) and viniferins are not specifically located on the same area when leaves are infected by Plasmopara viticola. Results obtained by MALDI mass spectrometry imaging demonstrate that this technique would be essential to improve the level of knowledge concerning the role of the stilbene phytoalexins involved in a stress event.

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Role of stilbenes in the resistance of grapevine to powdery mildew

Cited by 132 publications

References 21 publications

The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera

The R2R3-MYB Transcription Factors MYB14 and MYB15 Regulate Stilbene Biosynthesis in Vitis vinifera

Response of Vitis vinifera cell cultures to Eutypa lata and Trichoderma atroviride culture filtrates: expression of defence-related genes and phenotypes

MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves

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