Role of STAT3 signaling pathway in repair of liver injury

Chen, Xin-Li

doi:10.11569/wcjd.v22.i8.1051

Cited by 2 publications

(2 citation statements)

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“…The JAK-STAT and activator of transcription signal transduction pathway is an important pathway that mediates the signal transduction of a variety of cytokines, and is involved in many aspects of the body's inflammatory response, immune response and immune cell differentiation. The STAT3 signaling pathway has been shown in recent studies to play a vital role in the recovery from liver injury, which can promote hepatocyte proliferation, regulate carbohydrate and lipid metabolism, and activate the expression of hepatoprotective acute-phase proteins [16] .…”

Section: Resultsmentioning

confidence: 99%

The Role and Mechanism of M2c Macrophages in Lipopolysaccharide/D-Galactosamine Induced Acute Hepatitis

Li¹,

Huang²,

Zheng³

et al. 2022

IJPS

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The study was based on the evaluation of the positive effect of M2c macrophages on liver tissue inflammation, pathological changes and tissue damage in lipopolysaccharide/D-galactosamine induced acute hepatitis. Meanwhile, whether M2c macrophages induced anti-inflammatory effects through Janus kinase/signal transducer and activator of transcription signaling pathway was also investigated. Forty institute of cancer research female mice in the same batch with no significant difference in body weight were distributed randomly into 5 different groups namely the control group, solvent control group, lipopolysaccharide/D-galactosamine, lipopolysaccharide/D-galactosamine+M2c and lipopolysaccharide/ D-galactosamine+M2c+ruxolitinib, 8 in each group. 6 h after the last treatment of mice in each group, serum was collected to detect the content of alanine aminotransferase and aspartate transferase. The pathology of liver tissue was determined using hematoxylineosin staining. The determination of the levels of expression of anti-inflammatory factors, pro-inflammatory factors, related apoptosis proteins and Janus kinase/signal transducer and activator of transcription signaling pathway related proteins in liver tissue were carried out by Western blot. After 6 h of induction with lipopolysaccharide/D-galactosamine, the liver function (alanine aminotransferase, aspartate aminotransferase), pathological score of liver slices, liver pro-inflammatory factors and anti-inflammatory factors (interleukin-6, tumor necrosis factor-alpha, interleukin-1beta and interleukin-10) were significantly improved in the mice blood. Comparative to the normal control group, apoptosis-related proteins (B-cell lymphoma-extra-large, B-cell lymphoma 2, Bcl-2associated X protein) and other indicators were higher with a significant difference (p<0.05) under the acute hepatitis model induced lipopolysaccharide/D-galactosamine, after infusion of M2c macrophages from the tail vein of mice. The above indexes of mice were improved and exhibited a statistically significant difference (p<0.05). After adding ruxolitinib to intervene Janus kinase/signal transducer and activator of transcription signaling pathway on the basis of lipopolysaccharide/D-galactosamine+M2c group, the liver function, liver slice pathological score, pro and anti-inflammatory factor and apoptosis-related protein expressions were significantly higher as compared to the lipopolysaccharide/D-galactosamine+M2c group with significant difference (p<0.05), while the observed differences were not significant (p>0.05) in the lipopolysaccharide/ D-galactosamine group. Lipopolysaccharide/D-galactosamine induces the occurrence of acute hepatitis, which can well mimic the virus the pathogenic process of hepatitis. M2c macrophages exhibited a critical anti-inflammatory role in acute hepatitis via activating the Janus kinase/signal transducer and activator of transcription signaling pathway.

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Section: Resultsmentioning

confidence: 99%

The Role and Mechanism of M2c Macrophages in Lipopolysaccharide/D-Galactosamine Induced Acute Hepatitis

Li¹,

Huang²,

Zheng³

et al. 2022

IJPS

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“…When liver cells are damaged, the expression of STAT3 increases following acute stress, the binding force to DNA strengthens, and the expression of Cyclin D1 is activated directly or indirectly. Cell mitosis then accelerates and stimulates liver cell proliferation ( 29 – 36 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of modified Xiaochaihu decoction-containing serum on HepG2.2.15 cells via the JAK2/STAT3 signaling pathway

Chen

Wang

Shichuan

et al. 2017

Molecular Medicine Reports

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The present study aimed to investigate the possible mechanisms underlying the effect of modified Xiaochaihu decoction (mXCHD) in the treatment of chronic hepatitis B (CHB). Patients with CHB, in addition to liver stagnation and spleen deficiency syndrome were randomly assigned to receive either Chinese (mXCHD) or western (entecavir) treatment, with 30 cases in each group. Serum was collected following treatment with mXCHD or entecavir for 7 days. A healthy group of 30 individuals was also included. HepG2.2.15 cells were cultured in vitro and randomly divided into four groups: Healthy; entecavir-treated; 10% mXCHD-treated; and 20% mXCHD-treated. The HepG2.2.15 cells in the four groups were treated with either serum from the healthy volunteers, entecavir-containing serum, or mXCHD-containing serum at different concentrations (10 or 20%, respectively). Following treatment with the corresponding serum, cell proliferation was examined using an MTT assay, and the expression of hepatitis B surface antigen (HBsAg) in the cell supernatant was detected using an enzyme-linked immunosorbent assay. The mRNA and protein expression levels of Janus kinase (JAK)2 and signal transducer and activator of transcription (STAT)3 were measured using reverse transcription-quantitative polymerase chain reaction and western blot analyses, respectively. The results indicated that the most effective treatment for the promotion of HepG2.2.15 cell proliferation was a 20% concentration of mXCHD serum. The expression of HBsAg was significantly decreased in the groups treated with 10 and 20% mXCHD 48 h following intervention (P<0.01). The mRNA and protein expression levels of STAT3 in the 20% mXCHD serum group were significantly increased, compared with those in the healthy group (P<0.01 and P<0.05, respectively), whereas no significant difference was observed in the expression of JAK2 among the four groups. These results indicated that mXCHD suppressed the hepatitis B virus, and treatment of the cells with mXCHD-containing serum promoted HepG2.2.15 cell proliferation via modulating the expression of STAT3, which may contribute to the clinical efficacy of mXCHD against CHB.

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Role of STAT3 signaling pathway in repair of liver injury

Cited by 2 publications

References 0 publications

The Role and Mechanism of M2c Macrophages in Lipopolysaccharide/D-Galactosamine Induced Acute Hepatitis

The Role and Mechanism of M2c Macrophages in Lipopolysaccharide/D-Galactosamine Induced Acute Hepatitis

Effect of modified Xiaochaihu decoction-containing serum on HepG2.2.15 cells via the JAK2/STAT3 signaling pathway

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