Role of spermine in mammalian sperm capacitation and acrosome reaction

Rubinstein, Sara; Breitbart, Haim

doi:10.1042/bj2780025

Cited by 42 publications

(29 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polyamines (putrescine, spermidine, and spermine) are arginine degradation products and are small cationic molecules with proliferative effects, required to regulate cell growth and inflammation and homeostasis. Lower spermine levels are associated with defects in the neck and body of sperm cells (midpiece defects) (Rubinstein & Breitbart, ). The supplementation of polyamine cursor of S‐adenosylmethionine was shown to increase sperm motility in patients with oligozoospermia (Vanella et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Metabolomics analysis of seminal plasma in patients with idiopathic Oligoasthenoteratozoospermia using high‐resolution NMR spectroscopy

et al. 2019

View full text Add to dashboard Cite

Background Male infertility is a global health issue caused by a combination of different factors. Specialists generally rely on semen analysis to diagnose male infertility. However, it is known that diagnostic semen analysis fails to identify about 50% of male infertility disorders. Recently, metabolomics has been proven to be a powerful technique for the diagnosis of different diseases. Objective To determine whether metabolites could be used as potential biomarkers for the diagnosis of male factor infertility through comparing seminal plasma samples from infertile men with oligoasthenoteratozospermia (OAT) and samples from normozoospermic controls. Materials and methods This study utilized high‐resolution 1H NMR spectroscopy to reveal whether the metabolomic changes of seminal plasma obtained from 31 patients with oligoasthenoteratozospermia (OAT) are different from the ones obtained from 28 normozoospermic controls. Results Multivariate statistical analysis of NMR data concluded that the metabolomic profile of samples from patients with OAT exhibits statistically significant differences when compared to the controls. The differences were based on the metabolites lactate, citrate, lysine, arginine, valine, glutamine, creatinine, α‐ketoglutaric acid, spermine, putrescine, and tyrosine. Except the tyrosine, levels of the above metabolites were significantly decreased in patients with OAT compared to the controls. The levels of citrate, choline, spermine, putrescine, α‐ketoglutaric acid, valine, and tyrosine were significantly different (p < 5 × 10−4) between two groups. On the other hand, levels of lactate, creatinine, lysine, arginine, and glutamine were also statistically significant (0.001 < p < 0.05). However, considering the p‐values, the physiological relevance of these metabolites may be lower when compared to the others. A PLS‐DA model built on the NMR data achieved 89.29% sensitivity and 93.55% specificity results in a leave‐one‐out cross‐validation process. Discussion and conclusion 1H NMR spectroscopy‐based metabolomic analysis could be used as a diagnostic tool for the diagnosis of oligoasthenoteratozospermia.

show abstract

Section: Discussionmentioning

confidence: 97%

Metabolomics analysis of seminal plasma in patients with idiopathic Oligoasthenoteratozoospermia using high‐resolution NMR spectroscopy

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Spermidine/spermine N1-acetyl transferase was implicated in spermine metabolism by catalysing the acetylation of spermidine and spermine (Della Ragione et al, 1983). It has been reported that spermine can inhibit the acrosome reaction, thereby preventing premature capacitation (Rubinstein and Breitbart, 1991), but its role in uterus has not been reported before. In the present study, the upregulation of spermidine/spermine N1-acetyl transferase expression at oestrus may adjust the ratio of acetylized spermine in the uterine cavity, thereby exerting a regulatory effect on sperm state.…”

Section: Discussionmentioning

confidence: 99%

Global gene profiling analysis of mouse uterus during the oestrous cycle

Li²,

Piao³

et al. 2003

Reproduction

View full text Add to dashboard Cite

Many genes related to the cyclic changes of the uterus during the oestrous cycle have been identified using a oneby-one approach. In the present study, cDNA microarray technology was applied to investigate the global profile of gene expression of mouse uterus at the oestrous and dioestrous stages. At a certain stage of the oestrous cycle, the uteri of mature CD-1 mice (n = 10) were removed, pooled and snap-frozen in liquid nitrogen. Total RNA was extracted to synthesize cDNA probes for microarray assay. By screening 8192 mouse genes and expressed sequence tags (ESTs), 51 upregulated and 51 downregulated genes were identified in oestrous uterus, of which 62 are well characterized and 40 are ESTs. The known genes were assigned to various gene categories according to their main function. The microarray was performed three times with three independent sets of uterine tissue pools. The results of northern blot analysis for small proline-rich protein 2 (Sprr2), 17␤-hydroxysteroid dehydrogenase type 2 (17␤HSD-2), high mobility group 2 (Hmg2), mitotic checkpoint component 2 (Mad2) and an EST AW555366 mRNA were consistent with that of microarray analysis. In situ hybridization was performed to localize the transcript of the EST AW555366. Most of the upregulated genes encode secreted immunerelated proteins, proteinases and their inhibitors, indicating their potential involvement in sperm viability as well as capacitation. The downregulated genes mainly encode cell cycle-related factors, implying the active proliferation of uterus at dioestrus.

show abstract

“…Spermine is present in seminal plasma in millimolar concentrations. Rubinstein and Breitbart have reported that at this concentration it inhibits the Ca'+-induced acrosomal reaction in vitro [44], possibly by blocking phosphoiipase C hydrolysis of phosphinositide. We have recently re-examined the effects of spermine on the acrosomal reaction and found that, at micromolar concentrations, spermine induces the acrosomal reaction in a Ca'+-independent manner (our unpublished results).…”

Section: Fees Leltersmentioning

confidence: 99%

A cell free system reveals that capacitation is a prerequisite for membrane fusion during the acrosome reaction

et al. 1992

View full text Add to dashboard Cite

Plasma and oukr acrosomal membranes were extracted from bovine spermatozoa and used in an in vitro fusion assay. Fusion was revealed by monitoring the merging of lipids using Ihe chlorophyll &V,N'-dioctadecyloxacarbocyanine-ptolucne sulfonate (DCY) method [(1984) Biochim. Biophys. Acta 769. 531-5421. The requirement for capacitation, as well as the effects of pi-i, calcium and @ermine, on membrane fusion in our cell-free system were similar to those observed in vivo on the acrosomal reaction. This demonstrates for the first time that capacitation and alterations in in~raccllular pH and calcium concentration, which must precede the acrosomal reaction, arc required for the membrane fusion event.

show abstract

Role of spermine in mammalian sperm capacitation and acrosome reaction

Cited by 42 publications

References 25 publications

Metabolomics analysis of seminal plasma in patients with idiopathic Oligoasthenoteratozoospermia using high‐resolution NMR spectroscopy

Metabolomics analysis of seminal plasma in patients with idiopathic Oligoasthenoteratozoospermia using high‐resolution NMR spectroscopy

Global gene profiling analysis of mouse uterus during the oestrous cycle

A cell free system reveals that capacitation is a prerequisite for membrane fusion during the acrosome reaction

Contact Info

Product

Resources

About