Role of Sperm Sulfogalactosylglycerolipid in Mouse Sperm-Zona Pellucida Binding1

White, David; Weerachatyanukul, Wattana; Gadella, Bart M.; Kamolvarin, Nuanthip; Attar, Mayssa; Tanphaichitr, Nongnuj

doi:10.1095/biolreprod63.1.147

Cited by 66 publications

(65 citation statements)

References 53 publications

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“…This is possible, since prosaposin is present in the oviductal fluid (M Schenk, DC Santos and N Tanphaichitr, unpublished observations) and cathepsin D, which is known for its processing activity of prosaposin to saposin B (Hiraiwa et al 1997), is also released as part of the AC (Erickson & Martin 1974). This SGG desulfation may be essential; acrosomal vesicles would still adhere to the ZP due to SGG affinity for the ZP (White et al 2000), thus impeding sperm migration through the ZP layer. On the other hand, galactosylglycerolipid (GG), the desulfated product of SGG, has minimal affinity for the ZP (White et al 2000) and would thus be released into the surrounding.…”

Section: Wtmentioning

confidence: 99%

“…This SGG desulfation may be essential; acrosomal vesicles would still adhere to the ZP due to SGG affinity for the ZP (White et al 2000), thus impeding sperm migration through the ZP layer. On the other hand, galactosylglycerolipid (GG), the desulfated product of SGG, has minimal affinity for the ZP (White et al 2000) and would thus be released into the surrounding. We are currently investigating these possibilities to gain a better understanding of ASA-ZP interaction.…”

Section: Wtmentioning

confidence: 99%

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Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner

Xu¹,

Liu²,

Srakaew³

et al. 2012

Reproduction

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We have shown previously that sperm surface arylsulfatase A (ASA) of mouse, pig, and human is involved in sperm-egg zona pellucida (ZP) binding. By treating capacitated mouse sperm with A23187 to induce the acrosome reaction, we demonstrated by immunoblotting that ASA also existed in the acrosomal content and on the inner acrosomal membrane. Since mZP2 and mZP3 are known as sperm receptors, whereas mZP1 as a cross-linker of mZP2/mZP3, we determined whether purified ASA bound to mZP2 and mZP3 selectively. The three mZP glycoproteins were purified from solubilized ovarian ZP by size exclusion column chromatography. Immuno-dot blot analyses revealed that purified sperm ASA bound to mZP2 at the highest level followed by mZP3, whereas the binding of ASA to mZP1 was minimal. The results confirmed the physiological significance of sperm ASA in the ZP binding process. The binding of ASA to mZP2 and mZP3 was, however, not dependent on the active site pocket amino acids, Cys69, Lys123, and Lys302, which are pertinent to the capturing of an arylsulfate substrate, since ASA mutant with Ala substitution at these three residues still bound to mZP2 and mZP3. The availability of the active site pocket of ASA bound to the ZP suggested that ASA would still retain enzymatic activity, which might be important for subsequent sperm penetration through the ZP.

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Section: Wtmentioning

confidence: 99%

Section: Wtmentioning

confidence: 99%

Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner

Xu¹,

Liu²,

Srakaew³

et al. 2012

Reproduction

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“…In addition, in sea urchin, sperm gangliosides have been shown to bind to sperm-binding proteins localized on the vitelline layer of the egg surface 34 . Furthermore, in mice, sperm sulfogalactosylglycerolipids have been shown to bind to the egg extracellular sulfoglycoprotein matrix, the zona pellucida 35 . These data suggest that sperm glycolipids are involved in fertilization.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Novel Rhamnose-containing Acidic Glycosphingolipid from the Ascidian <i>Haloc</i><i>ynthia aurantium</i>

et al. 2017

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“…As mentioned above, a raft lipid component, SGG, is a ZP adhesion molecule (23,41). Proteomic analyses reveal that a number of proteins known for their affinity for or association with the ZP are also present in isolated sperm lipid rafts, including PH-20 (46,47), basigin (46), spermadhesins (48), proacrosin (48), TESP1 (46), TESP5 sn (49) and N-acetylglucosamine:beta-1,4-galactosyltranferase (47).…”

Section: E Lipid Rafts In Capacitated Sperm As Platforms For Egg Intmentioning

confidence: 99%

“…Besides these biochemical characterizations, we have shown that the functional properties of isolated sperm rafts are partially dependent on SGG. We have demonstrated that detergent resistant lipid rafts of capacitated pig sperm have the ability to bind to the egg zona pellucida glycoproteins (ZP), and this binding ability is partially attributed to SGG affinity for the ZP (23,41). SGG is also present in plasma membrane vesicles of the sperm head anterior, isolated from live sperm by nitrogen cavitation.…”

Section: Cmarkers For Sperm Lipid Raftsmentioning

confidence: 99%

Lipid Rafts and Sulfogalactosylglycerolipid (SGG) in Sperm Functions: Consensus and Controversy

Tanphaichitr

Faull²,

Yaghoubian³

et al. 2007

TIGG

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Lipid rafts are ordered membrane microdomains e n r i c h e d i n c h o l e s t e r o l , g l y c o l i p i d s a n d s a t u r a t e d phospholipids. Lipid rafts also assemble molecules involved in cell adhesion and signaling, and they are thus considered platforms for these phenomena. This has made the lipid raft topic a very active research field. However, a number of issues remain controversial in this research line. Advanced imaging studies have recently indicated that individual lipid/membrane rafts are of nanometer sizes. This suggests that rafts isolated from cells as low-density membrane fractions may rather represent "macrorafts" due to coalescence of individual rafts. Further controversy lies in the use of Triton X-100 in raft isolation, as the detergent may induce lipid microdomain formation. Investigators in the field are moving towards using milder non-ionic detergents or physical forces in raft isolation. Sperm-egg interaction is an ideal system to attest the implication of lipid rafts in cell adhesion/signaling. Recently, we have shown that Triton X-100 resistant membrane rafts of capacitated pig sperm have direct binding to pig zona pellucida (ZP) with a K d value much lower than that of noncapacitated sperm rafts-ZP binding. These results may partially explain the enhanced ZP binding ability of capacitated sperm, relative to non-capacitated sperm. Another possible contribution to the greater ZP binding ability of capacitated sperm is their possession of higher lipid raft levels despite a cholesterol efflux occurring during capacitation. Significantly, GM1 ganglioside, normally used as a raft marker of somatic cells, does not exist in capacitated sperm rafts. Rather, 70% of male germ cell specific sulfogalacotosylglycerolipid (SGG) is present in isolated capacitated sperm rafts, making it an attractive candidate as a sperm raft marker. Nonetheless, since sperm lipid rafts used in these studies were isolated by the Triton X-100 treatment method, work should be repeated using lipid rafts prepared from sperm by a physical force (e.g., nitrogen cavitation). Finally, advanced imaging studies

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Role of Sperm Sulfogalactosylglycerolipid in Mouse Sperm-Zona Pellucida Binding1

Cited by 66 publications

References 53 publications

Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner

Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner

Characterization of a Novel Rhamnose-containing Acidic Glycosphingolipid from the Ascidian <i>Haloc</i><i>ynthia aurantium</i>

Lipid Rafts and Sulfogalactosylglycerolipid (SGG) in Sperm Functions: Consensus and Controversy

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