Role of sonic hedgehog signaling in migration of cell lines established from CD133-positive malignant glioma cells

Uchida, Hiroyuki; Arita, Kazunori; Yunoue, Shunji; Yonezawa, Hajime; Shinsato, Yoshinari; Kawano, Hiroto; Hirano, Hirofumi; Hanaya, Ryosuke; Tokimura, Hiroshi

doi:10.1007/s11060-011-0552-2

Cited by 43 publications

(33 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Использование циклопамина и ингибиторов HH-сигналинга ведёт к уменьшению сферообразования, уменьшению пролиферации клеток и уменьшению уровня GLI1 [50,60,61]. При этом дополнительное добавление лиганда Shh приводило к увеличению сферообразования, экспрессии SMO, GLI1 и GLI2 [60,62].…”

Section: изменения сигнального пути Hedgehog при глиомахunclassified

“…Считается, что такие клетки контролируют развитие опухоли, обладают ограниченной способностью к делению и являются наиболее химиорезистентными клетками [60,62]. Опухолевые стволовые клетки характеризуются наличием определенных маркеров, основным из которых считается CD133.…”

Section: влияние на опухолевые стволовые клеткиunclassified

“…Белок Shh увеличивает, а циклопамин уменьшает образование туморосфер; это свидетельствует в пользу того, что сигнальный путь HH участвует в формировании фенотипа опухолевых стволовых клеток при раке молочной железы. Кроме того, гиперэкспрессия GLI1 или GLI2 увеличивает способность клеток к сферообразованию [60,62,82].…”

Section: влияние на опухолевые стволовые клеткиunclassified

“…Очень интересным феноменом представляется подтвёржденная многими исследователями активация пути HH в стволоподобных опухолевых клетках, образующих туморосферы [60,62,82]. Об этом свидетельствует повышенное содержание Shh и SMO в этих клетках.…”

Section: заключениеunclassified

See 3 more Smart Citations

Hedgehog signaling in the pathogenesis of neuro-oncology diseases

et al. 2015

View full text Add to dashboard Cite

The review summarizes current knowledge on the Hedgehog signaling pathway, its role in normal embryogenesis and/or initiation and progression of neuro-oncological diseases, especially of high-grade gliomas, the most malignant neuroepithelial tumors. The main proteins forming the Hedgehog signaling pathway include Shh, PTCH1, SMO, HHIP, SUFU and GLI1 isoforms. Effects of other signaling pathways on the family of transcription factors GLI and other proteins are described. The review summarizes modern data about the impact of the Hedgehog signaling pathway on proliferation, migration activity and invasiveness, and also on tumor neoangiogenesis and tumor cell chemoresistance. The role of the Hedgehog signaling pathway in origin of cancer stem cells and epithelial-mesenchymal transition is also analyzed. Some prospects for new anticancer drugs acting on components of the Hedgehog signaling pathway inhibitors are demonstrated.

show abstract

Section: изменения сигнального пути Hedgehog при глиомахunclassified

Section: влияние на опухолевые стволовые клеткиunclassified

Section: заключениеunclassified

See 2 more Smart Citations

Hedgehog signaling in the pathogenesis of neuro-oncology diseases

et al. 2015

View full text Add to dashboard Cite

show abstract

“…A study conducted by Uchida et al (10) described that purified CD133 + cells generated neurospheres in culture, and differentiated into neurons and glial cells that were capable of cell differentiation. Other groups have also verified that, contrary to CD133 -cells, CD133 + cells were capable of inducing brain tumors in in vivo models (11,12) .…”

Section: Introductionmentioning

confidence: 99%

Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma

Pavon

Marti

Sibov

et al. 2012

Einstein (São Paulo)

View full text Add to dashboard Cite

Objective: To establish the method of isolation and culture of human glioblastoma neurospheres, and the purification of their stem cells, followed by the process of obtaining tumor subspheres, immunophenotypically characterizing this clonogenic set. Methods: Through the processing of glioblastoma samples (n=3), the following strategy of action was adopted: (i) establish primary culture of glioblastoma; (ii) isolation and culture of tumor neurospheres; (iii) purify cells that initiate tumors (CD133 + ) by magnetic separation system (MACS); (iv) obtain tumor subspheres; (v) study the expression of the markers nestin, CD133, and GFAP. Results: The study successfully described the process of isolation and culture of glioblastoma subspheres, which consist of a number of clonogenic cells immunophenotypically characterized as neural, which are able to initiate tumor formation. Conclusion: These findings may contribute to a better understanding of the process of gliomagenesis.

show abstract

CD133 expression in circulating tumor cells from breast cancer patients: Potential role in resistance to chemotherapy

Nadal

Ortega

Salido

et al. 2013

Intl Journal of Cancer

View full text Add to dashboard Cite

CD133 has been associated with cell properties such as self renewal, migration and vasculogenic mimicry, potentially involved in generation of circulating tumor cells (CTCs). We characterized CD133 expression in CTCs of 98 nometastatic breast cancer (BC) patients. CTCs were isolated by immunomagnetic techniques using magnetic beads labeled with a multicytokeratin(CK)-specific antibody (CK3-11D5) and CTCs and CD133 detection through immunocytochemical methods. CK 1 /CD133 1 CTCs were identified in 65% of patients at baseline and 47.8% after systemic therapy (p 5 0.53). Correlation of CD133 status in CTCs with classical clinicopathological characteristics and response to therapy was performed. Her2 not amplified and low Ki-67 index were positively correlated with presence of CK 1 /CD133 1 CTCs. Before any treatment, CK 1 /CD133 1 CTCs were more frequently isolated in patients with luminal BC subtype. No statistically significant differences were found between proportion of CK 1 /CD133 1 CTCs and BC subtypes after systemic therapy, implying a relative enrichment of CK 1 /CD133 1 CTCs in triple negative and HER2-amplified tumors. While CK 1 /CTCs decreases after chemotherapy when analyzing the whole population, CK 1 / CD133 1 CTCs were enriched in post-treatment samples in nonluminal BC subtypes. These findings suggest the potential role of CD133 as a promising marker of chemoresistance in nonluminal BC patients. Further prospective studies and extensive preclinical modeling will be needed to confirm whether CD133 is a marker of resistance to chemotherapy, and its role as a target for novel anticancer therapies targeting cancer stem cells and tumor vasculature.Breast cancer (BC) is a complex and heterogeneous disease comprising multiple tumor entities associated with distinctive different biological feature, clinical behaviors and response to therapy. The current classification of BC is based on the pattern of expression of the hormone receptors (HR) estrogen receptor and/or progesterone receptor, and human epidermal receptor 2 (Her2) that identify three major BC subtypes: luminal tumors, which are HR positive and Her2 negative; Her2 amplified tumors; and those tumors with lack of expression of the three receptors, referred to as triple negative (TN) BC. 1 Despite advances in BC treatments, primary and acquired resistance to cancer therapies remains a challenge especially in nonmetastatic patients. Cancer stem cells (CSC) have been

show abstract

Role of sonic hedgehog signaling in migration of cell lines established from CD133-positive malignant glioma cells

Cited by 43 publications

References 32 publications

Hedgehog signaling in the pathogenesis of neuro-oncology diseases

Hedgehog signaling in the pathogenesis of neuro-oncology diseases

Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma

CD133 expression in circulating tumor cells from breast cancer patients: Potential role in resistance to chemotherapy

Contact Info

Product

Resources

About