Role of sodium ions for sulfate transport and energy metabolism in Desulfovibrio salexigens

Kreke, Bernd; Cypionka, Heribert

doi:10.1007/bf00248893

Cited by 20 publications

(17 citation statements)

References 23 publications

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“…Uptake of different ions was determined by measuring the changes in light scattering in cell suspensions (Mitchell & Moyle, 1969 ;Varma et al, 1983 ;Kreke & Cypionka, 1994). Cells were suspended at OD %$' 1n0 (light path l 1 cm) in 2 ml 175 mM KCl with 10 mM MOPS buffer (pH 7n0).…”

Section: Methodsmentioning

confidence: 99%

“…Cells (0n725 mg protein ml − ") were incubated on ice in a solution of 320 mM NaCl, 80 mM KCl and 10 mM MgCl # (pH 9n5). Twenty microlitres of 0n1 M HCl (resulting in a pH shift of 2 units), or pulses of NaCl or KCl (both 0n52 M), were then added ; subsamples of 100 µl were analysed for ATP as described by Kreke & Cypionka (1994).…”

Section: Bioenergetics Of D Hydrogenovoransmentioning

confidence: 99%

“…To investigate which ion was translocated by the ATPase, proton or sodium-ion pulses were added (Kreke & Cypionka, 1994). Pulses of Na + (0n52 M NaCl) to resting cells did not result in ATP formation, whereas the ATP level of the cells increased sixfold upon HCl pulses, shifting the pH from 9n2 to 7n0.…”

Section: Dependency Of Atp Synthesis On Protons or Sodium Ionsmentioning

confidence: 99%

“…In neutrophilic sulfate reducers, sodium-dependent transport of sulfate was found in marine, but usually not in freshwater, strains (Stahlmann et al, 1991 ;Cypionka, 1995). Electrogenic Na + -H + antiporters have been detected in freshwater and marine sulfate reducers (Varma et al, 1983 ;Kreke & Cypionka, 1994). The present study was carried out in order to characterize the energy metabolism of Desulfonatronovibrio hydrogenovorans by means of physiological experiments.…”

Section: Introductionmentioning

confidence: 98%

“…Sodium-driven ATP conservation has also been shown for another new alkaliphilic sulfate reducer, Desulfonatronum lacustre (Pikuta et al, 1998 ;Pusheva et al, 1999Pusheva et al, , 2000. In contrast to this species, all neutrophilic sulfate reducers studied so far conserve ATP by proton translocation (Fitz & Cypionka, 1989 ;Kreke & Cypionka, 1994). An inverse pH gradient appears less critical if energy conservation is coupled to sodium cycling.…”

Section: Introductionmentioning

confidence: 99%

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Bioenergetics of the alkaliphilic sulfate-reducing bacterium Desulfonatronovibrio hydrogenovorans

et al. 2002

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Energy metabolism of the alkaliphilic sulfate-reducing bacterium Desulfonatronovibrio hydrogenovorans strain Z-7935 was investigated in continuous culture and in physiological experiments on washed cells. When grown in chemostats with H 2 as electron donor, the cells had extrapolated growth yields [Y max , g dry cell mass (mol electron acceptor) N1 ] of 55 with sulfate and 128 with thiosulfate. The maintenance energy coefficients were 19 and 13 mmol (g dry mass) N1 h N1 , and the minimum doubling times were 27 and 20 h with sulfate and thiosulfate, respectively. Cell suspensions reduced sulfate, thiosulfate, sulfite, elemental sulfur and molecular oxygen in the presence of H 2 . In the absence of H 2 , sulfite, thiosulfate and sulfur were dismutated to sulfide and sulfate. Sulfate and sulfite were only reduced in the presence of sodium ions, whereas sulfur was reduced also in the absence of Na M . Plasmolysis experiments showed that sulfate entered the cells via an electroneutral symport with Na M ions. The presence of an electrogenic Na M -H M antiporter was demonstrated in experiments applying monensin (an artificial electroneutral Na M -H M antiporter) and propylbenzylylcholine mustard.HCl (a specific inhibitor of Na M -H M antiporters). Sulfate reduction was sensitive to uncouplers (protonophores), whereas sulfite reduction was not affected. Changes in pH upon lysis of washed cells with butanol indicated that the intracellular pH was lower than the optimum pH for growth (pH 95). Pulses of NaCl (052 M) to cells incubated in the absence of Na M did not result in ATP formation, whereas HCl pulses (shifting the pH from 92 to 70) did. Small oxygen pulses, which were reduced within a few seconds, caused a transient alkalinization. The results of preliminary experiments with chemiosmotic inhibitors provided further evidence that the alkalinization was caused by sodium-proton antiport following a primary electron-transport-driven sodium ion translocation. It is concluded that energy conservation in D. hydrogenovorans depends on a proton-translocating ATPase, whereas electron transport appears to be coupled to sodium ion translocation.

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Section: Methodsmentioning

confidence: 99%

Section: Bioenergetics Of D Hydrogenovoransmentioning

confidence: 99%

Section: Dependency Of Atp Synthesis On Protons or Sodium Ionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Bioenergetics of the alkaliphilic sulfate-reducing bacterium Desulfonatronovibrio hydrogenovorans

et al. 2002

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Sulfate biosensor for environmental applications

Marzocchi

Revsbech

2022

Limnology & Ocean Methods

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Sulfate is present in all freshwater and marine environments and is reduced to toxic and corrosive hydrogen sulfide by anaerobic bacteria. By measuring depth profiles of sulfate in sediments, it is possible to obtain estimates of sulfate reduction rates (SRRs). A whole‐cell microscale biosensor for sulfate was constructed by placing a mixture of sulfate‐reducing and aerobic bacteria in front of a hydrogen sulfide microsensor. The bacteria were supplied with electron donors from a reservoir behind the bacterial biomass. The aerobic bacteria ensured anaerobic conditions in deeper parts of the 150‐μm‐thick bacterial layer, so that the sulfate reducers could be active. A typical tip diameter of a sulfate biosensor is 50 μm. The calibration curve was close to linear in the 0–2 mM range, with a detection limit of about 10 μM. The 90% response time varied between 90 and 220 s, slowest response at low concentrations. Oxygen and ferrous iron at environmental concentrations gave no interference, but there was a 6% signal difference between stirred and stagnant liquid medium. The sensitivity of the biosensor could be modulated by applying a charge between sensor interior and an external electrode. The lifetime is usually a few weeks. The biosensor was used to measure sulfate profiles in a freshwater sediment.

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Solute Transport and Cell Energetics

Cypionka

1995

Sulfate-Reducing Bacteria

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Role of sodium ions for sulfate transport and energy metabolism in Desulfovibrio salexigens

Cited by 20 publications

References 23 publications

Bioenergetics of the alkaliphilic sulfate-reducing bacterium Desulfonatronovibrio hydrogenovorans

Bioenergetics of the alkaliphilic sulfate-reducing bacterium Desulfonatronovibrio hydrogenovorans

Sulfate biosensor for environmental applications

Solute Transport and Cell Energetics

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