Role of siRNAs and miRNAs in the processes of RNA-mediated gene silencing during viral infections

Krulko, I.; Ustyanenko, D.; Polischuk, V. P.

doi:10.3103/s0095452709010113

Cited by 2 publications

(1 citation statement)

References 99 publications

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“…RNA silencing plays an important antiviral role in plants and animals [6][7][8][9]. In plants, ds replicative intermediates of RNA viruses or highly structured single-stranded (ss) viral RNAs may be processed by DCLs and virus specific sRNAs incorporated into RISC target genomic (g) and subgenomic (sg) RNAs for cleavage, thus reducing the virus level in infected cells.…”

Section: Introductionmentioning

confidence: 99%

The Citrus leaf blotch virus movement protein acts as silencing suppressor

et al. 2011

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To counteract plant antiviral defense based on RNA silencing, many viruses express proteins that inhibit this mechanism at different levels. The genome of Citrus leaf blotch virus (CLBV) encodes a 227-kDa protein involved in replication, a 40-kDa movement protein (MP), and a 41-kDa coat protein (CP). To determine if any of these proteins might have RNA silencing suppressor activities, we have used Agrobacterium-mediated transient assays in the green fluorescent protein (GFP)-expressing Nicotiana benthamiana line 16c. Only CLBV MP was able to suppress intracellular GFP silencing induced by expression of either single- or double-stranded (ds) GFP RNA, but not cell-to-cell or long distance spread of the silencing signal. The MP suppressor activity was weak compared to other characterized viral suppressor proteins. Overall our data indicate that MP acts as a suppressor of local silencing probably by interfering in the silencing pathway downstream of the steps of dsRNA and small RNAs generation.

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Section: Introductionmentioning

confidence: 99%