Role of siderophores in cystic fibrosis pathogenesis: Foes or friends?

Mossialos, Dimitris; Amoutzias, Grigoris D.

doi:10.1016/j.ijmm.2008.06.008

Cited by 25 publications

(26 citation statements)

References 99 publications

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“…We and others have reported that P. aeruginosa can affect the growth and also the expression of some virulence factors of Burkholderia spp. (Costello et al 2014;Tomlin et al 2001;Mossialos and Amoutzias 2009;Bakkal et al 2010;Tashiro et al 2010;McKenney et al 1995). Ornibactin production was significantly increased by B. cenocepacia J2315 in the presence of P. aeruginosa supernatant, with a concomitant inhibition of growth and subsequently PVD, the predominant siderophore produced by P. aeruginosa, was shown to be a significant inducer of this siderophore response.…”

Section: Discussionmentioning

confidence: 92%

“…Cystic fibrosis (CF) is an autosomal genetic disorder and is the most common lethal genetic disease among Caucasians affecting approximately 1:2500 people worldwide (Mossialos and Amoutzias 2009;Jacquot et al 2008). A defect in the cystic fibrosis transmembrane conductance regulator (CFTR) gene causes an increase in mucus viscosity in the CF lung which impairs mucociliary clearance of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the multifaceted iron acquisition strategies of Burkholderia cenocepacia

Tyrrell¹,

Whelan²,

Wright³

et al. 2015

Biometals

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Burkholderia cenocepacia is a bacterial pathogen which causes severe respiratory infections in cystic fibrosis (CF). These studies were aimed at gaining an insight into the iron acquisition strategies of B. cenocepacia. In iron restricted conditions, genes associated with the synthesis and utilisation of ornibactin (pvdA, orbA, orb F) were significantly upregulated compared to the expression of pyochelin associated genes (pchD, fptA). In the absence of alternative iron sources, B. cenocepacia J2315 and 715j utilised ferritin and haemin, but not transferrin or lactoferrin for growth. Significantly, mutants unable to produce ornibactin, (715j-orbI) or ornibactin and pyochelin, (715j-pobA), utilised haemin and ferritin more efficiently than the wild-type. Moreover, both mutants were also able to utilise lactoferrin for growth (P ≤ 0.01) and additionally 715j-pobA utilised transferrin (P ≤ 0.01), potentially facilitating adaptation to the host environment. Furthermore, B. cenocepacia increased ornibactin gene expression in response to pyoverdine from Pseudomonas aeruginosa (P ≤ 0.01), demonstrating the capacity to compete for iron in co-colonised niches. Pyoverdine also significantly diminished the growth of B. cenocepacia (P < 0.001) which was related to its iron chelating activity. In a study of three B. cenocepacia sequential clonal isolates obtained from a CF patient over a 3.5 year period, ornibactin upregulation in response to pyoverdine was less pronounced in the last isolate compared to the earlier isolates, as was growth in the presence of haemin and ferritin, indicating alternative iron acquisition mechanism(s) may dominate as chronic infection progresses. These data demonstrate the multifaceted iron acquisition strategies of B. cenocepacia and their capacity to be differentially activated in the presence of P. aeruginosa and during chronic infection.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Investigation of the multifaceted iron acquisition strategies of Burkholderia cenocepacia

Tyrrell¹,

Whelan²,

Wright³

et al. 2015

Biometals

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“…However, these infection models are quite different from our study, as cyanide toxicity was evaluated after needle pricking of Drosophila , and not oral infection. Similarly, siderophores are often important for survival inside the host (Schryvers and Stojiljkovic, 1999; Ratledge, 2007; Mossialos and Amoutzias, 2009), but none of the two siderophores produced by P. entomophila (Matthijs et al ., 2009) is required for its virulence (data not shown). However, we mostly looked at the overall pathogenicity of the secondary metabolite mutants; hence we can imagine that a closer look at intestinal damages might reveal subtler phenotypes.…”

Section: Discussionmentioning

confidence: 99%

A secondary metabolite acting as a signalling molecule controls Pseudomonas entomophila virulence

Vallet-Gély

Opota

Boniface

et al. 2010

Cellular Microbiology

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SummaryPseudomonas entomophila is an entomopathogenic bacterium that is lethal to Drosophila melanogaster within 1-2 days of ingestion of high doses. Flies orally infected with P. entomophila rapidly succumb despite the induction of both local and systemic immune responses. Recent studies suggest that its virulence relies on its ability to cause irreversible damages to the intestinal epithelium, in contrast to what is observed with milder pathogenic bacteria such as Erwinia carotovora carotovora Ecc15 or Pseudomonas aeruginosa PA14. The GacS/GacA two-component system plays a key role in P. entomophila pathogenicity. Here, we report the identification of the pvf genes, whose products are involved in production of a secondary metabolite involved in P. entomophila virulence. A pvf mutant is impaired in its ability to persist within the gut, to trigger the fly immune responses and to inflict gut damages. The expression of several genes is affected in a pvf mutant, independently of the Gac system. Moreover, growing a pvf mutant in medium supplemented with supernatant extracts from either the wild-type strain or a gacA mutant restore its pathogenicity. Collectively, our results indicate that we identified genes involved in the synthesis of a signalling molecule that controls P. entomophila virulence independently from the Gac system.

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“…Clonal relationship. Bacterial typing was conducted by REP-PCR with primers REP1I and REP2I for repetitive extragenic palindromic sequences (Moura et al 2007). One bacterial colony was suspended in 25 μl of sterile distilled water and heated at 98°C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of The Pathogenic Potential of Insecticidal Serratia marcescens Strains to Humans

Konecka

Mokracka

Krzymińska

et al. 2019

Polish Journal of Microbiology

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We observed the death of insect caterpillars of Spodoptera exigua in the laboratory culture line and identified Serratia marcescens as the bacterial causative agent of the insect death. We confirmed that S. marcescens had insecticidal activity against S. exigua and caused high mortality of larvae. The LC50 values of S. marcescens CFU per 1cm2 of insect diet surface were similar for all isolates. Our research reports novel strains with high pesticidal activity as candidates for future research on a new bioinsecticide. As bioinsecticides cannot be harmful to non-target organisms, we determined the pathogenic properties of S. marcescens to humans. We proved the ability of S. marcescens todamage mammalian epithelial cells. All strains had cytopathic effects to Vero cells with a cytotoxic index ranging from 51.2% ± 3.8%to 79.2% ± 4.1%. We found that all of the strains excreted catecholate siderophore – enterobactin. All isolates were resistant to sulfamethoxazole, tobramycin, gentamicin, cefepime, and aztreonam. We did not observe the ESBL phenotype and the integrons’ integrase genes. Resistance to sulfamethoxazole was due to the presence of the sul1 or sul2 gene. The use of resistant S. marcescens strains that are pathogenic to humans in plant protection may cause infections difficult to cure and lead to the spread of resistance genes. The results of our study emphasize the necessity of determination of the safety to vertebrates of the bacteria that are proposed to serve as biocontrol agents. The novelty of our study lies in the demonstration of the indispensability of the bacteria verification towards the lack of hazardous properties to humans.

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Role of siderophores in cystic fibrosis pathogenesis: Foes or friends?

Cited by 25 publications

References 99 publications

Investigation of the multifaceted iron acquisition strategies of Burkholderia cenocepacia

Investigation of the multifaceted iron acquisition strategies of Burkholderia cenocepacia

A secondary metabolite acting as a signalling molecule controls Pseudomonas entomophila virulence

Evaluation of The Pathogenic Potential of Insecticidal Serratia marcescens Strains to Humans

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