Role of shear stress on biofilm formation of Candida krusei in a rotating disk system

Brugnoni, Lorena Inés; Cubitto, María Amelia; Lozano, Jorge

doi:10.1016/j.jfoodeng.2010.08.029

Cited by 16 publications

(13 citation statements)

References 38 publications

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“…Flocs that are important in successful wastewater treatment were removed and then a mixed supernatant, comprising planktonic bacteria, was cultured for 24 h before it was inoculated into the high-shear bioreactor. The clustering patterns observed were also observed in previous studies [73][74][75], but the positive and strong correlation between clustering and shear is novel and the level of turbulence in the flow developed to induce such high shear flows is unique in biofilm colonisation studies. The mechanisms suggested to be important for the clustering patterns are co-adhesion [76], which occurs on the substratum and describes the propensity for bacteria to attach in proximity to those that have already attached, and coaggregation [77], which is the coming-together of bacteria in clumps in the bulk water prior to adhesion onto the surface.…”

Section: Discussionsupporting

confidence: 78%

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

Tsagkari

Keating

Couto

et al. 2017

Water

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Abstract:The structure of biofilms in drinking water systems is influenced by the interplay between biological and physical processes. Bacterial aggregates in bulk fluid are important in seeding biofilm formation on surfaces. In simple pure and co-cultures, certain bacteria, including Methylobacterium, are implicated in the formation of aggregates. However, it is unclear whether they help to form aggregates in complex mixed bacterial communities. Furthermore, different flow regimes could affect the formation and destination of aggregates. In this study, real drinking water mixed microbial communities were inoculated with the Methylobacterium strain DSM 18358. The propensity of Methylobacterium to promote aggregation was monitored under both stagnant and flow conditions. Under stagnant conditions, Methylobacterium enhanced bacterial aggregation even when it was inoculated in drinking water at 1% relative abundance. Laminar and turbulent flows were developed in a rotating annular reactor. Methylobacterium was found to promote a higher degree of aggregation in turbulent than laminar flow. Finally, fluorescence in situ hybridisation images revealed that Methylobacterium aggregates had distinct spatial structures under the different flow conditions. Overall, Methylobacterium was found to be a key strain in the formation of aggregates in bulk water and subsequently in the formation of biofilms on surfaces.

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Section: Discussionsupporting

confidence: 78%

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

Tsagkari

Keating

Couto

et al. 2017

Water

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“…Many studies report the biofilm-forming potential of Candida spp. in food processing lines, e.g., for C. krusei, C. albicans and C. tropicalis (Brugnoni, Cubitto, & Lozano, 2012;Brugnoni et al, 2011;Kumamoto, 2002). As a viable alternative, natural antimicrobial compounds have received attention, mainly due the increase in microorganism resistance and tolerance to several food processing and preservation methods (Gyawali & Ibrahim, 2014).…”

Section: Antifungal Activitymentioning

confidence: 99%

“…In a recent review, Negi (2012) describes several species with antimicrobial potential for use in food safety applications, ascribing the effects to specific chemical classes (e.g., essential oils, flavonoids, tannins, alkaloids). Biofilm formation in food processing lines has become problematic and is a frequent source of infections (Brugnoni, Cubitto, & Lozano, 2011;Srey, Jahid, & Ha, 2013). This problem is studied with a focus on food equipment maintenance, the understanding of biofilm formation and preventive actions (Brugnoni, Lozano, & Cubitto, 2007;Stoodley, Cargo, Rupp, Wilson, & Klapper, 2002).…”

Section: Introductionmentioning

confidence: 99%

Ecdysteroids in Sida tuberculata R.E. Fries (Malvaceae): Chemical composition by LC–ESI-MS and selective anti-Candida krusei activity

Rosa

Camargo

et al. 2015

Food Chemistry

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“…The Rotating Disk System (RDS) used in the present work was described in Brugnoni et al (2011b) (Figure 1). A 0.2 m diameter, 5 mm thickness acrylic disk was rotated in a SS container (30 cm in diameter and 19 cm in height).…”

Section: Biofilm Formation In Turbulent Flowmentioning

confidence: 99%

“…One of these yeasts, a strain of Candida krusei, was capable of colonizing stainless steel and forming biofilms under laminar, transitional, and turbulent flow (Brugnoni et al 2011a(Brugnoni et al , 2011b2012). To the authors' knowledge, there are no published studies on the rheology of yeast biofilms and how it is influenced by the growth environment.…”

Section: Introductionmentioning

confidence: 99%

In siturheology of yeast biofilms

et al. 2014

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The aim of the present work was to investigate the in situ rheological behavior of yeast biofilms growing on stainless steel under static and turbulent flow. The species used (Rhodototula mucilaginosa, Candida krusei, Candida kefyr and Candida tropicalis) were isolated from a clarified apple juice industry. The flow conditions impacted biofilm composition over time, with a predominance of C. krusei under static and turbulent flow. Likewise, structural variations occurred, with a tighter appearance under dynamic flow. Under turbulent flow there was an increase of 112 μm in biofilm thickness at 11 weeks (p < 0.001) and cell morphology was governed by hyphal structures and rounded cells. Using the in situ growth method introduced here, yeast biofilms were determined to be viscoelastic materials with a predominantly solid-like behavior, and neither this nor the G'0 values were significantly affected by the flow conditions or the growth time, and at large deformations their weak structure collapsed beyond a critical strain of about 1.5-5%. The present work could represent a starting point for developing in situ measurements of yeast rheology and contribute to a thin body of knowledge about fungal biofilm formation.

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Role of shear stress on biofilm formation of Candida krusei in a rotating disk system

Cited by 16 publications

References 38 publications

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

Ecdysteroids in Sida tuberculata R.E. Fries (Malvaceae): Chemical composition by LC–ESI-MS and selective anti-Candida krusei activity

In siturheology of yeast biofilms

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