Role of shear stress on composition, diversity and dynamics of biofilm bacterial communities

Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system. Water Research, 47 (2). 503 -516. ISSN 0043-1354 https://doi.org/10.1016/j.watres.2012.09.053 eprints@whiterose.ac.uk https://eprints.whiterose.ac.uk/ Reuse Unless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request.

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“…We have detected differences in bacterial community composition within biofilms after response to higher detachment forces (Rochex et al, 2008).…”

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confidence: 88%

Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system

2013

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“…The effect of environmental conditions on biofilm formation, their structure, composition and physical proper-ties have been reported by Cloete et 3 al. (2003), Derlon et al (2008) and Rochex et al (2008).…”

Section: Introductionmentioning

confidence: 99%

The effect of shear stress on the formation and removal of Bacillus cereus biofilms

Lemos

Mergulhão

Melo

et al. 2015

Food and Bioproducts Processing

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A b s t r a c tThe influence of the shear stress (rw) under which biofilms were formed was assessed on their susceptibility to removal when exposed to chemical and mechanical stresses. A rotating cylinder reactor was used to form biofilms, allowing the simulation of rw conditions similar to those found in industrial settings, particularly in areas with low rw like elbows, corners, valves and dead zones. Bacillus cereus was used as a model bacterium for biofilm formation.Biofilms were formed on AISI316 stainless steel cylinders under different rw (estimated at 0.02, 0.12 and 0.17 Pa) for 7 days. Some phenotypic characteristics, including thickness, biomass production, cellular density and extracellular proteins and polysaccharides content were assessed. Biofilm density was found to increase significantly with rw while the thickness decreased. Also, biofilms formed at 0.02 Pa had lowest biomass content, cell density and extracellular polysaccharide content. Those characteristics were not statistically different for the biofilms formed under 0.12 and 0.17 Pa.Ex situ tests were performed by treating the biofilms with the biocide benzyldimethyldodecyl ammonium chloride (BDMDAC), followed by exposure to increasing rw conditions, up to 1.84 Pa (whereas the maximum rw used during growth was 0.17 Pa). The biofilms formed under low rw were more resistant to removal caused by the BDMDAC action alone. Those formed under higher rw were more resistant to the mechanical and the combined chemical and mechanical treatments. The amount of biofilm remaining on the cylinders, after both treatments was statistically similar for biofilms formed under 0.12 and 0.17Pa. The resistance of biofilms to removal by mechanical treatment (alone and combined with BDMDAC) was related to the amount of matrix polysaccharides.However, none of the methods investigated were able to remove all the biofilm from the cylinders. IntroductionThere is a lack of efficient strategies to clean stagnant zones in industrial plants (Brooks and Flint, 2008). Crevices, corners, dead zones, valves or areas where the mixing rate is low are almost inevitable. Stagnation promotes bacterial accumulation, ultimately leading to biofouling (Manuel et al., 2010). Biofouling is a damaging problem, affecting the energetic efficiency of industrial processes, causing corrosion of the surfaces, decreasing product quality and eventually promoting the spread of pathogens and resistant infectious diseases (Costerton et al., 1999;Ludensky, 2003;Srey et al., 2013). In industrial settings, surface disinfection is usually focused on the use of biocides, aiming to inactivate the microorganisms (Cloete et al., 1998;Faille et al., 2013). Since biofilms are complex biological structures adhered to a sur-face, these strategies often fail, as the removal of biomass is neglected. Hence, cleaning the biomass from the surfaces is fundamental for controlling biofilm development (Flemming, 2011).In the biofilm formation process, the hydrodynamic conditions define the transport of the cells, oxyg...

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“…Spinning disk rheometry has been used to load biofilms in shear (Vinogradov et al 2004). With regards to methods using hydrodynamic loading, bacterial biofilms are subjected to a fluid flow such as in flow cells (Stoodley et al 1999a(Stoodley et al , b, 2001a or Couette-Taylor type reactors (Coufort et al 2007;Rochex et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Advances in Biofilm Mechanics

Guélon¹,

Mathias²,

Stoodley

2011

Springer Series on Biofilms

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A knowledge of the mechanical properties of bacterial biofilms is required to more fully understand how a biofilm will physically respond, and adapt, to the physical forces, such as those caused by fluid flow or particle or bubble impingement, acting upon it. This is particularly important since biofilms are problematic in a wide diversity of scenarios and spatial and temporal scales and many control strategies designed to remove biofilms include a mechanical component such as fluid flow, particle or bubble impingement or a physical contact with the surface generated by scraping or brushing. Knowing when, and how, a biofilm might fail (through adhesive or cohesive failure) will allow better prediction of accumulation and biomass detachment, key processes required in the understanding of the structure and function of biofilm systems. However, the measurements of mechanical properties are challenging. Biofilms are living systems and they readily desiccate if removed from the liquid medium, it is not clear how quickly their mechanical properties might change when removed from their indigenous environment into a testing environment. They are also very thin and are inherently attached to a surface. They cannot be formed into standard test coupons such as plastics or solids, and cannot readily be poured or placed into conventional viscometers or rheometers, such as liquids and gels. Measured parameters such as the elastic and shear modulus, adhesive strength or tensile strength are sparse but are increasingly appearing in the literature. There is a large range of reported values for these properties, although there is general agreement that biofilms are viscoelastic. Biofilms have been assessed with various experimental methods depending on the desired characteristic and available equipment. The aforementioned challenges and T.

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Role of shear stress on composition, diversity and dynamics of biofilm bacterial communities

Cited by 192 publications

References 33 publications

Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system

Influence of hydraulic regimes on bacterial community structure and composition in an experimental drinking water distribution system

The effect of shear stress on the formation and removal of Bacillus cereus biofilms

Advances in Biofilm Mechanics

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