Role of serotonin in zebrafish (Danio rerio) anxiety: Relationship with serotonin levels and effect of buspirone, WAY 100635, SB 224289, fluoxetine and para-chlorophenylalanine (pCPA) in two behavioral models

Maximino, Caio; Puty, Bruna; Benzecry, Rancés; Araújo, Juliana Baldan Costa Neves; Lima, Mônica Gomes; Batista, Evander de Jesus Oliveira; Oliveira, Karen Renata de Matos; Crespo-López, María Elena; Herculano, Anderson Manoel

doi:10.1016/j.neuropharm.2013.03.006

Cited by 173 publications

(154 citation statements)

References 83 publications

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“…For example, anxiolytics cause zebrafish to switch their preference from the dark to the light zone [43]. In our study, we used the selective GABA A antagonist gabazine as an anxiety-generating (anxiogenic) compound, which caused control rockfish to prefer the dark zone, thus establishing that, for rockfish, dark preference in the light/dark test is indicative of increased anxiety.…”

Section: Discussionmentioning

confidence: 96%

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

2014

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The average surface pH of the ocean is dropping at a rapid rate due to the dissolution of anthropogenic CO 2 , raising concerns for marine life. Additionally, some coastal areas periodically experience upwelling of CO 2 -enriched water with reduced pH. Previous research has demonstrated ocean acidification (OA)-induced changes in behavioural and sensory systems including olfaction, which is due to altered function of neural gamma-aminobutyric acid type A (GABA A ) receptors. Here, we used a camera-based tracking software system to examine whether OA-dependent changes in GABA A receptors affect anxiety in juvenile Californian rockfish (Sebastes diploproa). Anxiety was estimated using behavioural tests that measure light/dark preference (scototaxis) and proximity to an object. After one week in OA conditions projected for the next century in the California shore (1125 + 100 matm, pH 7.75), anxiety was significantly increased relative to controls (483 + 40 matm CO 2 , pH 8.1). The GABA A -receptor agonist muscimol, but not the antagonist gabazine, caused a significant increase in anxiety consistent with altered Cl 2 flux in OA-exposed fish. OA-exposed fish remained more anxious even after 7 days back in control seawater; however, they resumed their normal behaviour by day 12. These results show that OA could severely alter rockfish behaviour; however, this effect is reversible.

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Section: Discussionmentioning

confidence: 96%

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

2014

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“…Consistent with that hypothesis, drugs which increase 5-HT levels in the zebrafish brain increase defensive behaviour in the light/dark test and decrease it in the novel tank test (Egan et al, 2009;Sackerman et al, 2010;Maximino et al, 2011Maximino et al, , 2013bMaximino et al, , 2014aIturriaga-Vásquez et al, 2012;Kyzar et al, 2013;Stewart et al, 2013;Herculano & Maximino, 2014;Cheng et al, 2016). Moreover, there is a correlation between 5-HT levels in the extracellular fluid ex vivo and behaviour in the light/dark test, with higher levels associated with more defensive behaviour in the light/dark test and less defensive behaviour in the novel tank test (Maximino et al, 2013b).…”

Section: Serotonin and The Aversive Behaviour Network Of Zebrafishmentioning

confidence: 80%

“…In both the light/dark and novel tank tests, the 5-HT1A receptor antagonist WAY 100,635 decreases defensive behaviour (Maximino et al, 2013b); however, the antagonist p-MPPF increases anxiety-like behaviour in the novel tank test (Nowicki et al, 2014). Antagonists at 5-HT2 and 5-HT3 receptors were shown to increase defensive behaviour in the novel tank test (Nowicki et al, 2014), while 5-HT1B receptor antagonists decrease it (Maximino et al, 2013b;Nowicki et al, 2014).…”

Section: Serotonin and The Aversive Behaviour Network Of Zebrafishmentioning

confidence: 94%

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

Soares

Maximino

2018

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Animal-focused research has been crucial for scientific advancement however, in this matter, rodents are still taking a starring role. Coming out from merely being supportive of evidence found in rodents, the use of fish models has slowly taken a more central role and expanded its overall contributions in areas such as social sciences, evolution, physiology, and recently in translational medical research. In neurosciences, zebrafish has been widely adopted, contributing to our understanding of the genetic control of brain processes, and the effects of pharmacological manipulations. However, discussion continues regarding the paradox of function versus structure, when fish and mammals are compared, and on the potentially evolutionarily conserved nature of behaviour across fish species. From the behavioural stand point we explored aversive/stress and social behaviour in selected fish models, and refer to the extensive contributions of stress and monoaminergic systems. We suggest that, in spite of marked neuroanatomical differences between fish and mammals, stress and sociality are conserved at the behavioural and molecular levels. We also suggest that stress and sociality are mediated by monoamines in predictable and non-trivial ways, and that monoamines could "bridge" the relationship between stress and social behaviour. To reconcile the level of divergence with the level of similarity, we need neuroanatomical, pharmacological, behavioural, and ecological studies conducted in the laboratory and in nature.These areas need to add to each other to enhance our understanding of fish behaviour and ultimately how this all may translate to better model systems for translational studies.

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“…Consistent with that hypothesis, drugs which increase 5-HT levels in the zebrafish brain increase anxiety-like behaviour in the light/dark test and decrease it in the novel tank test (Egan et al, 2009;Sackerman et al, 2010;Maximino et al, 2011Maximino et al, , 2013bMaximino et al, , 2014aIturriaga-Vásquez et al, 2012;Kyzar et al, 2013;Stewart et al, 2013;Herculano & Maximino, 2014;Cheng et al, 2016). Moreover, there is a correlation between 5-HT levels in the extracellular fluid ex vivo and behaviour in the light/dark test, with higher levels associated with more anxiety-like behaviour in the light/dark test and lower anxiety-like behaviour in the novel tank test (Maximino et al, 2013b).…”

Section: Monoaminergic Innervation In Teleostsmentioning

confidence: 81%

“…In both the light/dark and novel tank tests, the 5-HT 1A receptor antagonist WAY 100,635 decreases anxiety-like behaviour (Maximino et al, 2013b); however, the antagonist p-MPPF increases anxiety-like behaviour in the novel tank test (Nowicki et al, 2014). Antagonists at 5-HT 2 and 5-HT 3 receptors were shown to increase anxietylike behaviour in the novel tank test (Nowicki et al, 2014), while 5-HT 1B receptor antagonists decrease it (Maximino et al, 2013b;Nowicki et al, 2014).…”

Section: Monoaminergic Innervation In Teleostsmentioning

confidence: 94%

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

Soares¹,

Maximino²

2018

Preprint

Self Cite

View full text Add to dashboard Cite

Animal-focused research has been crucial for scientific advancement however, in this matter, rodents are still taking a starring role. Coming out from merely being supportive of evidence found in rodents, the use of fish models has slowly taken a more central role and expanded its overall contributions in areas such as social sciences, evolution, physiology, and recently in translational medical research. In neurosciences, zebrafish has been widely adopted, contributing to our understanding of the genetic control of brain processes, and the effects of pharmacological manipulations. However, discussion continues regarding the paradox of function versus structure, when fish and mammals are compared, and on the potentially evolutionarily conserved nature of behaviour across fish species. From the behavioural stand point we explorted aversive/stress and social behaviour in selected fish models, and refer to the extensive contributions of stress and monoaminergic systems. We suggest that, in spite of marked neuroanatomical differences between fish and mammals, stress and sociality are conserved at the behavioural and molecular levels. We also suggest that stress and sociality are mediated by monoamines in predictable and non-trivial ways, and that monoamines could “bridge” the relationship between stress and social behaviour. To reconcile the level of divergence with the level of similarity, neuroanatomy, pharmacology, behavioural analysis, and ecology studies conducted in the laboratory and in nature need to add to each other and enhance our understanding of fish behaviour and ultimately how this all may translate to better model systems for translational studies.

show abstract

Role of serotonin in zebrafish (Danio rerio) anxiety: Relationship with serotonin levels and effect of buspirone, WAY 100635, SB 224289, fluoxetine and para-chlorophenylalanine (pCPA) in two behavioral models

Cited by 173 publications

References 83 publications

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

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Role of serotonin in zebrafish (Danio rerio) anxiety: Relationship with serotonin levels and effect of buspirone, WAY 100635, SB 224289, fluoxetine and para-chlorophenylalanine (pCPA) in two behavioral models

Cited by 173 publications

References 83 publications

CO2-induced ocean acidification increases anxiety in Rockfish via alteration of GABAAreceptor functioning

CO2-induced ocean acidification increases anxiety in Rockfish via alteration of GABAAreceptor functioning

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

The Integration of Sociality, Monoamines, and Stress Neuroendocrinology in Fish Models: Applications in the Neurosciences

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Product

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CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning

CO₂-induced ocean acidification increases anxiety in Rockfish via alteration of GABA_Areceptor functioning