Role of SERCA and the sarcoplasmic reticulum calcium content on calcium waves propagation in rat ventricular myocytes

Salazar-Cantú, Ayleen; Pérez-Treviño, Perla; Montalvo-Parra, María Dolores; Balderas-Villalobos, Jaime; Gómez-Viquez, Norma Leticia; Garcı́a, Noemı́; Altamirano, Julio

doi:10.1016/j.abb.2016.05.018

Cited by 6 publications

(2 citation statements)

References 83 publications

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“…ISO, as a non‐selective beta agonist, increases heart rate, cardiac contractile force and comfortable tension when it acts on the β1 adrenoceptor. ISO also induces diastole in blood vessels and the trachea by acting on the β2 adrenoceptor 33,34 . Chronic β1 adrenoceptor stimulation further induces myocardial hypertrophy.…”

Section: Discussionmentioning

confidence: 99%

Isosteviol sodium protects the cardiomyocyte response associated with the SIRT1/PGC‐1α pathway

Mei

Liu

et al. 2020

J Cellular Molecular Medi

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Cardiomyocyte dysfunction is attributed to excess oxidative damage, but the molecular pathways involved in this process have not been completely elucidated. Evidence indicates that isosteviol sodium (STVNa) has cardioprotective effects. We therefore aimed to identify the effect of STVNa on cardiomyocytes, as well as the potential mechanisms involved in this process. We established two myocardial hypertrophy models by treating H9c2 cells with high glucose (HG) and isoprenaline (ISO). Our results showed that STVNa reduced H9c2 mitochondrial damage by attenuating oxidative damage and altering the morphology of mitochondria. The results also indicated that STVNa had a positive effect on HG‐ and ISO‐induced damages via mitochondrial biogenesis. The protective effects of STVNa on cardiomyocytes were associated with the regulation of the SIRT1/PGC‐1α signalling pathway. Importantly, the effects of STVNa involved different methods of regulation in the two models, which was confirmed by experiments using an inhibitor and activator of SIRT1. Together, the results provide the basis for using STVNa as a therapy for the prevention of cardiomyocyte dysfunctions.

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Section: Discussionmentioning

confidence: 99%

Isosteviol sodium protects the cardiomyocyte response associated with the SIRT1/PGC‐1α pathway

Mei

Liu

et al. 2020

J Cellular Molecular Medi

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“…In addition to governing contractile events, dysregulation of intracellular Ca 2+ also undergoes derangements that promote arrhythmogenesis through Ca 2+ -dependent and coupled electrophysiological effects. Aberrant Ca 2+ signals can modulate CaMKII activity which in turn regulates the activity of a variety of ion channels and transporters, for examples Nav1.516, RyR2, and SERCA2a1718.…”

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confidence: 99%

Modification of distinct ion channels differentially modulates Ca2+ dynamics in primary cultured rat ventricular cardiomyocytes

Shen

Zhao

et al. 2017

Sci Rep

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Primary cultured cardiomyocytes show spontaneous Ca2+ oscillations (SCOs) which not only govern contractile events, but undergo derangements that promote arrhythmogenesis through Ca2+ -dependent mechanism. We systematically examined influence on SCOs of an array of ion channel modifiers by recording intracellular Ca2+ dynamics in rat ventricular cardiomyocytes using Ca2+ specific fluorescence dye, Fluo-8/AM. Voltage-gated sodium channels (VGSCs) activation elongates SCO duration and reduces SCO frequency while inhibition of VGSCs decreases SCO frequency without affecting amplitude and duration. Inhibition of voltage-gated potassium channel increases SCO duration. Direct activation of L-type Ca2+ channels (LTCCs) induces SCO bursts while suppressing LTCCs decreases SCO amplitude and slightly increases SCO frequency. Activation of ryanodine receptors (RyRs) increases SCO duration and decreases both SCO amplitude and frequency while inhibiting RyRs decreases SCO frequency without affecting amplitude and duration. The potencies of these ion channel modifiers on SCO responses are generally consistent with their affinities in respective targets demonstrating that modification of distinct targets produces different SCO profiles. We further demonstrate that clinically-used drugs that produce Long-QT syndrome including cisapride, dofetilide, sotalol, and quinidine all induce SCO bursts while verapamil has no effect. Therefore, occurrence of SCO bursts may have a translational value to predict cardiotoxicants causing Long-QT syndrome.

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Cell micropatterning reveals the modulatory effect of cell shape on proliferation through intracellular calcium transients

Tong

Wang

et al. 2017

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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The mechanism by which cell shape regulates the function of the cell is one of the most important biological issues, but it remains unclear. Here, we investigated the effect of the regulation of cell shape on proliferation by using a micropatterning approach to confine MC3T3-E1 cells into specific shapes. Our results show that the proliferation rate for rectangle-, triangle-, square- and circle-shaped osteoblasts increased sequentially and was related to the nuclear shape index (NSI) but not the cell shape index (CSI). Interestingly, intracellular calcium transients also displayed different patterns, with the number of Ca peaks increasing with the NSI in shaped cells. Further causal investigation revealed that the gene expression levels of the inositol 1,4,5-triphosphate receptor 1 (IP3R1) and sarco/endoplasmic reticulum Ca-ATPase 2 (SERCA2), two major calcium cycling proteins in the endoplasmic reticulum (ER), were increased with an increase in NSI as a result of nuclear volume changes. Moreover, the down-regulation of IP3R1 and/or SERCA2 using shRNAs in circle-shaped or control osteoblasts resulted in changes in intracellular calcium transient patterns and cell proliferation rates towards that of smaller-NSI-shaped cells. Our results indicate that changes in cell shape changed nuclear morphology and then the gene expression of IP3R1 and SERCA2, which produced different intracellular calcium transient patterns. The patterns of intracellular calcium transients then determined the proliferation rate of the shaped osteoblasts.

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Role of SERCA and the sarcoplasmic reticulum calcium content on calcium waves propagation in rat ventricular myocytes

Cited by 6 publications

References 83 publications

Isosteviol sodium protects the cardiomyocyte response associated with the SIRT1/PGC‐1α pathway

Isosteviol sodium protects the cardiomyocyte response associated with the SIRT1/PGC‐1α pathway

Modification of distinct ion channels differentially modulates Ca2+ dynamics in primary cultured rat ventricular cardiomyocytes

Cell micropatterning reveals the modulatory effect of cell shape on proliferation through intracellular calcium transients

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