Role of sensitization routes in the development of type I hypersensitivity to natural rubber latex in mice

Woolhiser, Michael R.; Munson, Albert E.; Meade, B. Jean

doi:10.1002/(sici)1097-0274(199909)36:1+<139::aid-ajim49>3.0.co;2-i

Cited by 8 publications

(6 citation statements)

References 13 publications

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“…2,4-dinitrofluorobenzene (DNFB) was used as a positive control for irritancy. Irritancy measurements were performed as previously described [ 13 ]. The thickness of the right and left ear pinnae of each mouse was measured using a modified engineer's micrometer (Mitutoyo Co.) before the first chemical administration and 24 hours following the final exposure.…”

Section: Methodsmentioning

confidence: 99%

Allergic Potential and Immunotoxicity Induced by Topical Application of 1-Chloro-4-(Trifluoromethyl)Benzene (PCBTF) in a Murine Model

et al. 2011

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The purpose of the studies in this paper was to evaluate the allergic potential, immunotoxicity, and irritancy of the occupationally relevant chemical, 1-chloro-4-(trifluoromethyl)benzene, also known as parachlorobenzotrifluoride (PCBTF), following dermal exposure in a murine model. Evaluation of the sensitization potential, conducted using the local lymph node assay (LLNA) at concentrations ranging from 50% to 100%, identified a dose-dependent increase in lymphocyte proliferation with a calculated EC3 value of 53.1%. While no elevations in total or specific IgE were observed after exposure to any concentration of the chemical, significant increases in IFN-γ protein production by stimulated draining lymphoid cells were observed, indicating a T-cell-mediated response. Dermal exposure to PCBTF was not found to alter the immune response to a T-cell-dependant antigen. These results demonstrate that PCBTF has the potential to induce allergic sensitization following dermal exposure and based on LLNA results would be classified as a weak sensitizer.

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Section: Methodsmentioning

confidence: 99%

Allergic Potential and Immunotoxicity Induced by Topical Application of 1-Chloro-4-(Trifluoromethyl)Benzene (PCBTF) in a Murine Model

et al. 2011

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“…Murine models have been used to assess allergenisity of latex proteins by various routes of administration. Different routes of latex exposure lead to different immunological responses in mouse (Woolhiser et al 1999 & 2000). Previous studies have induced sensitization via respiratory, subcutaneous, intraperitoneal and dermal exposures (Meade & Woolhiser 2002).…”

Section: Discussionmentioning

confidence: 99%

A Latex‐Induced Allergic Airway Inflammation Model in Mice

Farzaneh

Hassan

Pourpak

et al. 2006

Basic Clin Pharma Tox

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Latex allergy is important due to serious health impacts and widespread use of its products. Latex allergic reactions can be induced in skin and mucosal surfaces including the respiratory tract. The development of murine models of allergic airway inflammation has provided a framework to dissect out the cellular and molecular mechanisms of allergic respiratory inflammation. In this study we have developed a new mouse model of latex allergic airway inflammation using aerosol inhalation. The allergic inflammatory responses were characterized in this model. Mice were injected intraperitoneally with 0, 10, 50, or 200 mg of latex extract and their serum anti-latex IgE titers were determined. In the second stage, a standard protocol of inhalation was designed and three doses of latex extract solutions including 1%, 0.1%, and 0.01% were used to induce allergic airway inflammation. Bronchoalveolar lavage cytokines (IL-5 and IL-13) and serum antilatex IgE and IgG 1 titers were determined by ELISA. Eosinophil levels in lung, peripheral blood, bronchoalveolar lavage and bone marrow were also evaluated. Histological analysis of lung tissue was also performed after latex inhalation. The aerosol inhalation of 1% latex allergens solution and presensitization with 50 mg of latex in this study resulted in the development of allergic airway inflammation characterized by elevated allergen specific IgE and IgG 1 , peripheral blood, bronchoalveolar lavage and bone marrow eosinophilia. Histological analysis of the lung revealed an inflammatory response characterized by eosinophil accumulation. Elevated levels of Th2 cytokines IL-5 and IL-13 also were shown in bronchoalveolar lavage samples. These studies demonstrate that sensitization and subsequent aerosol inhalational challenge of latex allergen extract promotes allergic airway inflammation characterized by elevated IL-5 and IL-13 and eosinophils.

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“…TDI (2.5%) was used as a positive control for the irritancy portion of the experiment. Irritancy measurements were performed as previously described (Woolhiser et al, 1999). The thickness of the right and left ear pinnae of each mouse was measured using a modified engineer's micrometer (Mitutoyo Co., Japan) before the first chemical administration and 24 h following the final exposure.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Furfuryl Alcohol Sensitization Potential Following Dermal and Pulmonary Exposure: Enhancement of Airway Responsiveness

Franko

Jackson

Hubbs

et al. 2011

Toxicological Sciences

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Furfuryl alcohol is considered by the U.S. Environmental Protection Agency to be a high volume production chemical, with over 1 million pounds produced annually. Due to its high production volume and its numerous industrial and consumer uses, there is considerable potential for work-related exposure, as well as exposure to the general population, through pulmonary, oral, and dermal routes of exposure. Human exposure data report a high incidence of asthma in foundry mold workers exposed to furan resins, suggesting potential immunologic effects. Although furfuryl alcohol was nominated and evaluated for its carcinogenic potential by the National Toxicology Program, studies evaluating its immunotoxicity are lacking. The studies presented here evaluated the immunotoxic potential of furfuryl alcohol following exposure by the dermal and pulmonary routes using a murine model. When tested in a combined irritancy local lymph node assay, furfuryl alcohol was identified to be an irritant and mild sensitizer (EC3 = 25.6%). Pulmonary exposure to 2% furfuryl alcohol resulted in enhanced airway hyperreactivity, eosinophilic infiltration into the lungs, and enhanced cytokine production (IL-4, IL-5, and interferon-γ) by ex vivo stimulated lung-associated draining lymphoid cells. Airway hyperreactivity and eosinophilic lung infiltration were augmented by prior dermal exposure to furfuryl alcohol. These results suggest that furfuryl alcohol may play a role in the development of allergic airway disease and encourage the need for additional investigation.

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Role of sensitization routes in the development of type I hypersensitivity to natural rubber latex in mice

Cited by 8 publications

References 13 publications

Allergic Potential and Immunotoxicity Induced by Topical Application of 1-Chloro-4-(Trifluoromethyl)Benzene (PCBTF) in a Murine Model

Allergic Potential and Immunotoxicity Induced by Topical Application of 1-Chloro-4-(Trifluoromethyl)Benzene (PCBTF) in a Murine Model

A Latex‐Induced Allergic Airway Inflammation Model in Mice

Evaluation of Furfuryl Alcohol Sensitization Potential Following Dermal and Pulmonary Exposure: Enhancement of Airway Responsiveness

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