Role of scaffold internal structure on in vivo bone formation in macroporous calcium phosphate bioceramics

Mastrogiacomo, Maddalena; Scaglione, Silvia; Martinetti, Roberta; Dolcini, L.; Beltrame, Francesco; Cancedda, Ranieri; Quarto, Rodolfo

doi:10.1016/j.biomaterials.2006.01.031

Cited by 455 publications

(345 citation statements)

References 48 publications

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“…respectively human expanded chondrocytes [17] and sheep bone marrow stromal cells (BMSC) [18]" The reference n. 16 …”

Section: ) Human Cells Were Used For the Cartilage Layer Bmscs Werementioning

confidence: 99%

“…respectively human expanded chondrocytes [17] and sheep bone marrow stromal cells (BMSC) [18]. pyruvate, 100 mM HEPES buffer, 100 U/mL penicillin, 100 µg/mL streptomycin, and 0.29 mg/ml L-glutamine (complete medium).…”

Section: Cell Isolation and Expansionmentioning

confidence: 99%

“…BMSC were cultured as described [18]. Mononuclear cells were plated at 5x10 6 in 100mm dishes in Coons modified Ham's F-12 medium supplemented with 10% FCS and 1ng/ml human recombinant FGF-2 (Austral Biologicals, San Ramon, CA).…”

Section: Cell Isolation and Expansionmentioning

confidence: 99%

“…BMSC/scaffold composites, in agreement and with the approval of the competent ethical committee and legal authorities, were subcutaneously implanted in immuno-deficient (ID) (CD-1 nu/nu) mice by using an established model of ectopic bone formation [18]. Recipient ID mice of 1 month of age, purchased from Charles River Italia, were kept in a controlled environment and given free access to food and water.…”

Section: Generation and Implantation Of Bmsc-scaffold Constructsmentioning

confidence: 99%

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Design of graded biomimetic osteochondral composite scaffolds

et al. 2008

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With the ultimate goal to generate suitable materials for the repair of osteochondral defects, in this work we aimed at developing composite osteochondral scaffolds organized in different integrated layers, with features which are biomimetic for articular cartilage and subchondral bone and can differentially support formation of such tissues.A biologically inspired mineralization process was first developed to nucleate Mg-doped hydroxyapatite crystals on type I collagen fibers during their self assembling. The resulting mineral phase was non-stoichiometric and amorphous, resembling chemico-physical features of newly deposited, natural bone matrix. A graded material was then generated, consisting of (i) a lower layer of the developed biomineralized collagen, corresponding to the subchondral bone, (ii) an upper layer of hyaluronic acid-charged collagen, mimicking the cartilaginous region, and (iii) an intermediate layer of the same nature as the biomineralized collagen, but with a lower extent of mineral, resembling the tidemark. The layers were stacked and freeze-dried, to obtain an integrated, monolithic composite. Culture of the material for 2 weeks after loading with articular chondrocytes yielded cartilaginous tissue formation selectively in the upper layer. Conversely, ectopic implantation in nude mice of the material after loading with bone marrow stromal cells resulted in bone formation which remained confined within the lower layer.In conclusion, we developed a composite material with cues which are biomimetic of an osteochondral tissue and with the capacity to differentially support cartilage and bone tissue generation. The results warrant test of the material as a substitute for the repair of osteochondral lesions in orthotopic animal models. Response to Reviewer #1:1) results and discussion should be separated We would prefer to maintain Results and Discussion combined, in order to avoid possible redundancies and improve readability. 2) figures should be detailed with arrows and information about specific orientations. Details and arrows have been included in the Figures3) collagen type 1 was used for the creation of the construct -in every layer? Usually collagen type 1 does not play a role in articular cartilage. This should be addressed in the discussion. According to the Reviewer's comment, a rationale for the use of type I collagen has been included in the text, as follows: "Type I collagen was selected for the synthesis of the composite layers, including the cartilaginous one, due to (i) its good physico-chemical stability and processability and (ii) high safety and biocompatibility profile, related to the removal of all Reply Letter to Editor and referee's comments telopeptides, which are potentially responsible for immunological reactions." (Section 2.1, Page 2, 1 st paragraph). Indeed, type II collagen is normally extracted porcine derivative, and is still not available either in bulk for processing or for clinical use. Nowadays there are only few papers on experimental studies desc...

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“…respectively human expanded chondrocytes [17] and sheep bone marrow stromal cells (BMSC) [18]" The reference n. 16 …”

Section: ) Human Cells Were Used For the Cartilage Layer Bmscs Werementioning

confidence: 99%

Section: Cell Isolation and Expansionmentioning

confidence: 99%

Section: Cell Isolation and Expansionmentioning

confidence: 99%

Section: Generation and Implantation Of Bmsc-scaffold Constructsmentioning

confidence: 99%

See 2 more Smart Citations

Design of graded biomimetic osteochondral composite scaffolds

et al. 2008

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“…It involves migration of several cell types [1], proliferation [2], differentiation [3], angiogenesis [4,5], and mineralization [6], as well as the interaction of numerous cytokines and growth factors [7]. Bone formation also depends on the architecture of the scaffold [8], its surface properties [9], the mechanical stimulation [10,11], the implantation site [12], and the boneescaffold interface [13]. Probing the effects of each parameter and their interaction has proven challenging.…”

Section: Introductionmentioning

confidence: 99%

Prediction of spatio-temporal bone formation in scaffold by diffusion equation

et al. 2011

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a b s t r a c tDeveloping a successful bone tissue engineering strategy entails translation of experimental findings to clinical needs. A major leap forward toward this goal is developing a quantitative tool to predict spatial and temporal bone formation in scaffold. We hypothesized that bone formation in scaffold follows diffusion phenomenon. Subsequently, we developed an analytical formulation for bone formation, which had only three unknown parameters: C, the final bone volume fraction, a, the so-called scaffold osteoconduction coefficient, and h, the so-called peri-scaffold osteoinduction coefficient. The three parameters were estimated by identifying the model within vivo data of polymeric scaffolds implanted in the femoral condyle of rats. In vivo data were obtained by longitudinal micro-CT scanning of the animals. Having identified the three parameters, we used the model to predict the course of bone formation in two previously published in vivo studies. We found the predicted values to be consistent with the experimental ones. Bone formation into a scaffold can then adequately be described through diffusion phenomenon. This model allowed us to spatially and temporally predict the outcome of tissue engineering scaffolds with only 3 physically relevant parameters.

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Calcium Phosphates and Nanocrystalline Apatites for Medical Applications

Victor

Sharma

2012

Integrated Biomaterials for Biomedical Technology

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Role of scaffold internal structure on in vivo bone formation in macroporous calcium phosphate bioceramics

Cited by 455 publications

References 48 publications

Design of graded biomimetic osteochondral composite scaffolds

Design of graded biomimetic osteochondral composite scaffolds

Prediction of spatio-temporal bone formation in scaffold by diffusion equation

Calcium Phosphates and Nanocrystalline Apatites for Medical Applications

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