Role of RpoS in stress resistance, biofilm formation and quorum sensing of
            <i>Shewanella baltica</i>

Zhang, C.; Wang, C.; Jatt, A.-N.; Liu, H.; Liu, Y.

doi:10.1111/lam.13424

Cited by 10 publications

(8 citation statements)

References 25 publications

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“…Previous studies indicated that flagella played a crucial role in the adhesion for bacteria, as the flic subunit promoted bacteria move to the attachment site in the early stage of adhesion (Sun et al, 2022). It has been observed in our previous study that RpoS negatively regulated the motility of S. baltica and the diameter of the rpoS mutant increased by 46.6% more than S. baltica wild type on the plate (Zhang et al, 2021). The flagella structure consists of three parts: a body basel, hook, and filament (Chilcott and Hughes, 2010).…”

Section: Flagellar Assembly and Biofilm Formationmentioning

confidence: 52%

“…The number of bacteria increased with storage time, and the growth curves of the two strains were very close, which almost overlapped after 4 days of storage with a viable count of about 8.2 log CFU/g, indicating that RpoS did not affect the growth of S. baltica (Figure 1A). Our previous study has shown that the deletion of rpoS does not affect the growth of S. baltica at neither 30 nor 4°C in LB broth (Zhang et al, 2021). Similarly, the growth of P. fluorescens did not change without rpoS (Liu et al, 2018).…”

Section: Resultsmentioning

confidence: 85%

“…The oxidoreductase activity and cofactor binding were the most represented. The increased motility has been observed in rpoS mutant (Zhang et al, 2021), which was mediated by flagellum. The changes in oxidoreductase indicated that RpoS may be involved in the activation of oxidative stress response of S. baltica.…”

Section: Go Analysismentioning

confidence: 83%

“…The S. baltica X7 was previously isolated from large yellow croaker. The corresponding rpoS mutant was constructed in the previous study (Zhang et al, 2021). Both of these two bacterial species were cultured in LB broth.…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

“…Feng et al (2021) demonstrated that sigma factors RpoS/RpoN regulated the stress response and spoilage activity of S. baltica. Our previous study indicated that RpoS controlled the stress resistance, quorum sensing and biofilm formation in S. baltica (Zhang et al, 2021), however, the detailed genes regulated by RpoS remain unknown.…”

Section: Introductionmentioning

confidence: 99%

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Sigma factor RpoS positively affects the spoilage activity of Shewanella baltica and negatively regulates its adhesion effect

Zhang

Chen²,

Pan³

et al. 2022

Front. Microbiol.

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Shewanella baltica is the dominant bacterium that causes spoilage of seafood. RpoS is an alternative sigma factor regulating stress adaptation in many bacteria. However, the detailed regulatory mechanism of RpoS in S. baltica remains unclear. This study aims to investigate the regulatory function of RpoS on spoilage activity and adhesion ability in S. baltica. Results revealed that RpoS had no effect on the growth of S. baltica, but positively regulated the spoilage potential of S. baltica accompanied by a slower decline of total volatile basic nitrogen, lightness, and the sensory score of fish fillets inoculated with rpoS mutant. RpoS negatively regulated the adhesion ability, which was manifested in that the bacterial number of rpoS mutant adhered to stainless steel coupon was higher than that of the S. baltica in the early stage, and the biofilm formed on glass slide by rpoS mutant was thicker and tighter compared with S. baltica. Transcriptomic analysis showed that a total of 397 differentially expressed genes were regulated by RpoS. These genes were mainly enrichment in flagellar assembly, fatty acid metabolism/degradation, and RNA degradation pathways, which were associated with motility, biofilm formation and cold adaptation. This study demonstrated that RpoS is a primary regulator involved in flagellar assembly mediated biofilm formation and cold adaptation-related spoilage activity of S. baltica. Our research will provide significant insights into the control of microbiological spoilage in seafood.

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Section: Flagellar Assembly and Biofilm Formationmentioning

confidence: 52%

Section: Resultsmentioning

confidence: 85%

Section: Go Analysismentioning

confidence: 83%

Section: Strains and Culture Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sigma factor RpoS positively affects the spoilage activity of Shewanella baltica and negatively regulates its adhesion effect

Zhang

Chen²,

Pan³

et al. 2022

Front. Microbiol.

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Diversity and distribution of culturable fouling bacteria in typical mariculture zones in Daya Bay, South China

Chen

Liu

et al. 2022

Arch Microbiol

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Shewanella spp. from wastewater treatment plant-affected environment: isolation and characterization

Popović

Kazazić

Bilić

et al. 2022

Environ Sci Pollut Res

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Bacteria from the genus Shewanella are inhabitants of marine and freshwater ecosystems, recognized fish spoilage bacteria, but less known as fish disease agents. Shewanella spp. isolated 2 from fish living in waters close to effluents of a wastewater treatment plant (WWTP) were not previously characterized. We have tested Shewanella isolates from WWTP-affected waters and related fish. Genotypic characterization identified most strains as S. baltica and S. oneidensis. In order to investigate the sensibility and accuracy of their MALDI-TOF MS identification, they were grown on two culture media enriched by various NaCl concentrations, incubated at different temperatures and duration. We analyzed their antimicrobial susceptibility on a panel of antimicrobial drugs and capacity for biofilm production. With a view to demonstrate their capacity to produce fatty acids, we assessed the impact of different culture media on their lipid profile. We performed zebrafish embryotoxicity tests to simulate the environmental infection of the earliest life stages in S. baltica-contaminated waters. The best MALDI-TOF MS identification scores were for strains cultivated on TSA for 24 h at 22 °C and with supplementation of 1.5% NaCl. Less than 17% of isolates demonstrated antimicrobial resistance.Most isolates were weak biofilm producers. Strain-to-strain variation of MIC and MBC was low.The major fatty acids were C15:0, C16:0, C16:1, C17:1, and iC15:0. Exposure of Danio rerio to different S. baltica concentrations induced severe effects on zebrafish development: decreased heartbeat rate, locomotor activity, and melanin pigmentation. S. baltica passed through chorionic pores of zebrafish.

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Role of RpoS in stress resistance, biofilm formation and quorum sensing of Shewanella baltica

Cited by 10 publications

References 25 publications

Sigma factor RpoS positively affects the spoilage activity of Shewanella baltica and negatively regulates its adhesion effect

Sigma factor RpoS positively affects the spoilage activity of Shewanella baltica and negatively regulates its adhesion effect

Diversity and distribution of culturable fouling bacteria in typical mariculture zones in Daya Bay, South China

Shewanella spp. from wastewater treatment plant-affected environment: isolation and characterization

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