Role of ROCK signaling in formation of the trophectoderm of the bovine preimplantation embryo

Negrón‐Pérez, Verónica M.; Rodrigues, L. T.; Mingoti, Gisele Zoccal; Hansen, Peter J.

doi:10.1002/mrd.22976

Cited by 20 publications

(17 citation statements)

References 9 publications

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“…However, the reverse effect was reported in bovines, which suggests a certain degree of variability among species (Negrón-Pérez et al 2018). Despite this difference, alterations in YAP or AMOT expression affect bovine blastocyst formation and the number of CDX2-positive cells is negatively correlated with TEAD4 expression level (Sakurai et al 2016, Negrón-Pérez et al 2018. While the functional link between cell polarization, activity of the Hippo signaling pathway, and CDX2 expression is conserved between mice and pigs and, by extension, other mammals, the molecular kinetic for TB lineage determination differs among species from that described in the mouse (Liu et al 2018).…”

Section: Blastocyst Development In Ungulates R153mentioning

confidence: 88%

“…As in the mouse, ROCK inhibition in pig embryos promotes Hippo signaling activity, suppressing CDX2 (Caudal type Homeobox 2) expression, a key transcription factor for TB specification and maintenance (Kono et al 2014, Liu et al 2018. However, the reverse effect was reported in bovines, which suggests a certain degree of variability among species (Negrón-Pérez et al 2018). Despite this difference, alterations in YAP or AMOT expression affect bovine blastocyst formation and the number of CDX2-positive cells is negatively correlated with TEAD4 expression level (Sakurai et al 2016, Negrón-Pérez et al 2018.…”

Section: Blastocyst Development In Ungulates R153mentioning

confidence: 99%

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Preimplantation development in ungulates: a ‘ménage à quatre’ scenario

2020

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In ungulates, early embryonic development differs dramatically from that of mice and humans and is characterized by an extended period of pre- and peri-implantation development in utero. After hatching from the zona pellucida, the ungulate blastocyst will stay free in the uterus for many days before implanting within the uterine wall. During this protracted peri-implantation period, an intimate dialog between the embryo and the uterus is established through a complex series of paracrine signals. The blastocyst elongates, leading to extreme growth of extra-embryonic tissues, and at the same time, the inner cell mass moves up into the trophoblast and evolves into the embryonic disc, which is directly exposed to molecules present in the uterine fluids. In the peri-implantation period, uterine glands secrete a wide range of molecules, including enzymes, growth factors, adhesion proteins, cytokines, hormones, and nutrients like amino and fatty acids, which are collectively referred to as histotroph. The identification, role, and effects of these secretions on the biology of the conceptus are still being described; however, the studies that have been conducted to date have demonstrated that histotroph is essential for embryonic development and serves a critical function during the pre- and peri implantation periods. Here, we present an overview of current knowledge on the molecular dialogue among embryonic, extraembryonic, and maternal tissues prior to implantation. Taken together, the body of work described here demonstrates the extent to which this dialog enables the coordination of the development of the conceptus with respect to the establishment of embryonic and extra-embryonic tissues as well as in preparation for implantation.

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Section: Blastocyst Development In Ungulates R153mentioning

confidence: 88%

Section: Blastocyst Development In Ungulates R153mentioning

confidence: 99%

Preimplantation development in ungulates: a ‘ménage à quatre’ scenario

2020

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“…Although the localization of specific transcription factors within ICM/TE and EPI/PE/TE lineages have been well studied during mammalian development, the upstream regulation of these critical factors is not fully understood (Paul & Knott 2014, Marcho et al 2015. Among the multiple signaling networks involved in early mammalian lineage specification, Hippo signaling has been shown to be involved in both TE (Strumpf et al 2005, Yagi et al 2007 and ICM (Wicklow et al 2014) specification, while roles for Notch (Rayon et al 2014) and ROCK signaling in TE fate acquisition have also been well supported (Kono et al 2014, Negron-Perez et al 2018. In addition, multiple factors are known to contribute to cell lineage segregation.…”

Section: Discussionmentioning

confidence: 99%

MCRS1 is essential for epiblast development during early mouse embryogenesis

et al. 2020

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Microspherule protein 1 (MCRS1, also known as MSP58) is an evolutionarily conserved protein that has been implicated in various biological processes. Although a variety of functions have been attributed to MCRS1 in vitro, mammalian MCRS1 has not been studied in vivo. Here we report that MCRS1 is essential during early murine development. Mcrs1 mutant embryos exhibit normal morphology at the blastocyst stage but cannot be recovered at gastrulation, suggesting an implantation failure. Outgrowth (OG) assays reveal that mutant blastocysts do not form a typical inner cell mass (ICM) colony, the source of embryonic stem cells (ESCs). Surprisingly, cell death and histone H4 acetylation analysis reveal that apoptosis and global H4 acetylation are normal in mutant blastocysts. However, analysis of lineage specification reveals that while the trophoblast and primitive endoderm are properly specified, the epiblast lineage is compromised and exhibits a severe reduction in cell number. In summary, our study demonstrates the indispensable role of MCRS1 in epiblast development during early mammalian embryogenesis.

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“…SOX17 is also expressed in ICM cells, however the expression profile of these cells indicates that this marker identifies cells committed to hypoblast in this species [3, 4, 6, 7]. In bovine embryos SOX17 protein expression has never been reported, however SOX17 mRNA was detected in presumptive hypoblast cells isolated from blastocysts that also expressed other well-known hypoblast markers GATA6, FGFR1, HNF4 and PDGFRα [8]. Studies exploring the roles of signalling pathways during lineage segregation showed that ERK modulation affects hypoblast development in human, pig and cattle embryos, although the effects are less pronounced than in mouse embryos [3, 5, 9–11].…”

Section: Introductionmentioning

confidence: 99%

A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos

et al. 2019

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Background The segregation of the hypoblast and the emergence of the pluripotent epiblast mark the final stages of blastocyst formation in mammalian embryos. In bovine embryos the formation of the hypoblast has been partially studied, and evidence shows that MEK signalling plays a limited role in the segregation of this lineage. Here we explored the role of different signalling pathways during lineage segregation in the bovine embryo using immunofluorescence analysis of NANOG and SOX17 as readouts of epiblast and hypoblast, respectively. Results We show that SOX17 starts to be expressed in 16–32-cell stage embryos, whereas NANOG is first detected from 8-cell stage. SOX17 is first co-expressed with NANOG, but these markers become mutually exclusive by the late blastocyst stage. By assessing the expression kinetics of NANOG/SOX17 we show that inhibition of MEK signalling can eliminate SOX17 expression in bovine blastocysts, without altering NANOG expression. Modulation of WNT, PKC and LIF did not affect NANOG expression in the epiblast when used in combination with the ERK inhibitor. Conclusions This study shows that SOX17 can be used as a reliable early marker of hypoblast in the bovine, and based on its expression profile we show that the hypoblast segregates in day 7 blastocysts. Furthermore, SOX17 expression is abolished using 1 μM of PD0325901, without affecting the NANOG population in the epiblast. Modulation of WNT, PKC and LIF are not sufficient to support enhanced NANOG expression in the epiblast when combined with ERK inhibitor, indicating that additional signalling pathways should be examined to determine their potential roles in epiblast expansion. Electronic supplementary material The online version of this article (10.1186/s12861-019-0193-9) contains supplementary material, which is available to authorized users.

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Role of ROCK signaling in formation of the trophectoderm of the bovine preimplantation embryo

Cited by 20 publications

References 9 publications

Preimplantation development in ungulates: a ‘ménage à quatre’ scenario

Preimplantation development in ungulates: a ‘ménage à quatre’ scenario

MCRS1 is essential for epiblast development during early mouse embryogenesis

A dose-dependent response to MEK inhibition determines hypoblast fate in bovine embryos

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