Role of RNA Splicing in Regulation of Cancer Stem Cell

Tripathi, Greesham; Tripathi, Avantika; Johnson, Joel M.; Kashyap, Manoj Kumar

doi:10.2174/1574888x16666211207103628

Cited by 5 publications

(2 citation statements)

References 34 publications

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“…CSCs are capable of undergoing cell division and, therefore, give rise to heterogeneity in the tumor, playing a crucial role in tumorigenesis. Characterization of CSCs properties can provide important information regarding tumor metastasis and drug resistance [ 109 , 110 ]. Chen et al [ 9 ] enriched metastatic breast cancer cells with microfluidics, and identified differentially expressed genes in metastatic cells via SCS.…”

Section: Scs and Tumor Metastasismentioning

confidence: 99%

Single-cell sequencing: a promising approach for uncovering the mechanisms of tumor metastasis

et al. 2022

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Single-cell sequencing (SCS) is an emerging high-throughput technology that can be used to study the genomics, transcriptomics, and epigenetics at a single cell level. SCS is widely used in the diagnosis and treatment of various diseases, including cancer. Over the years, SCS has gradually become an effective clinical tool for the exploration of tumor metastasis mechanisms and the development of treatment strategies. Currently, SCS can be used not only to analyze metastasis-related malignant biological characteristics, such as tumor heterogeneity, drug resistance, and microenvironment, but also to construct metastasis-related cell maps for predicting and monitoring the dynamics of metastasis. SCS is also used to identify therapeutic targets related to metastasis as it provides insights into the distribution of tumor cell subsets and gene expression differences between primary and metastatic tumors. Additionally, SCS techniques in combination with artificial intelligence (AI) are used in liquid biopsy to identify circulating tumor cells (CTCs), thereby providing a novel strategy for treating tumor metastasis. In this review, we summarize the potential applications of SCS in the field of tumor metastasis and discuss the prospects and limitations of SCS to provide a theoretical basis for finding therapeutic targets and mechanisms of metastasis.

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Section: Scs and Tumor Metastasismentioning

confidence: 99%

Single-cell sequencing: a promising approach for uncovering the mechanisms of tumor metastasis

et al. 2022

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“…RNA splicing and the protein machinery that guides this process, the spliceosome, constitute a very relevant biological target for cancer therapy [ 1 , 2 ]. Others and we have discovered small molecule leads that modulate the spliceosome and induce apoptosis preferentially in cancer cells [ 1 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Modulation of RNA splicing associated with Wnt signaling pathway using FD-895 and pladienolide B

Kumar

Kashyap

et al. 2022

Aging

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Alterations in RNA splicing are associated with different malignancies, including leukemia, lymphoma, and solid tumors. The RNA splicing modulators such as FD-895 and pladienolide B have been investigated in different malignancies to target/modulate spliceosome for therapeutic purpose. Different cell lines were screened using an RNA splicing modulator to test in vitro cytotoxicity and the ability to modulate RNA splicing capability via induction of intron retention (using RT-PCR and qPCR). The Cignal Finder Reporter Array evaluated [pathways affected by the splice modulators in HeLa cells. Further, the candidates associated with the pathways were validated at protein level using western blot assay, and gene-gene interaction studies were carried out using GeneMANIA. We show that FD-895 and pladienolide B induces higher apoptosis levels than conventional chemotherapy in different solid tumors. In addition, both agents modulate Wnt signaling pathways and mRNA splicing. Specifically, FD-895 and pladienolide B significantly downregulates Wnt signaling pathway-associated transcripts (GSK3β and LRP5) and both transcript and proteins including LEF1, CCND1, LRP6, and pLRP6 at the transcript, total protein, and protein phosphorylation's levels. These results indicate FD-895 and pladienolide B inhibit Wnt signaling by decreasing LRP6 phosphorylation and modulating mRNA splicing through induction of intron retention in solid tumors.

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RNA splicing: a dual-edged sword for hepatocellular carcinoma

Kashyap

Tripathi

et al. 2022

Med Oncol

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Role of RNA Splicing in Regulation of Cancer Stem Cell

Cited by 5 publications

References 34 publications

Single-cell sequencing: a promising approach for uncovering the mechanisms of tumor metastasis

Single-cell sequencing: a promising approach for uncovering the mechanisms of tumor metastasis

Modulation of RNA splicing associated with Wnt signaling pathway using FD-895 and pladienolide B

RNA splicing: a dual-edged sword for hepatocellular carcinoma

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