Role of RNA m6A modification in titanium dioxide nanoparticle-induced acute pulmonary injury: An in vitro and in vivo study

In situ bioorthogonal-modulation of m6A RNA methylation in macrophages for efficient eradication of intracellular bacteria

“…Research demonstrated that reactive oxygen species (ROS) could signicantly induce m 6 A levels. [50][51][52] To determine the ability of CIDM to generate ROS, we observed the change of absorption at 652 nm in the presence of the substrate 3,3 0 ,5,5 0 -tetramethylbenzidine (TMB). The absorption increased aer the addition of CIDM, proving that CIDM possessed the activity to produce ROS (Fig.…”

“…Interestingly, the constructed CIDM displayed OXD-mimic activity, which could facilitate the modulation of m 6 A methylation. [50][51][52] As a proof of concept, we applied this strategy to reprogram macrophage polarization into the bactericidal M1 phenotype for efficient bacterial ablation. 15,16,53,54 Such a bioorthogonal chemistrycontrolled epigenetics regulatory strategy could achieve a maximized treatment outcome and a minimized drug side effect, which provided an important insight into the development of in situ controllable m 6 A methylation.…”