Role of RNA‑binding protein 5 in the diagnosis and chemotherapeutic response of lung cancer (Review)

Xu, Yanling; Su, Zheng; Li, Junyao; Wang, Qi; Meng, Guangping; Yang, Wen; Zhang, Jie; Gao, Peng

doi:10.3892/ol.2018.9818

Cited by 9 publications

(9 citation statements)

References 81 publications

(104 reference statements)

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“…The function of RBM5 in neurogenesis is poorly understood (Jackson & Kochanek 2020), although its roles in apoptosis and tumour suppression (Xu et al 2019; Zhang et al 2019; Sutherland et al 2010; Bechara et al 2013) and spermatogenesis (O’Bryan et al 2013) are well documented. We show a clear switch from a truncated, non-coding RBM5 isoform ENST00000474470 to the full-length coding isoform ENST00000347869 during differentiation (Fig.…”

Section: Resultsmentioning

confidence: 99%

Long read sequencing reveals novel isoforms and insights into splicing regulation during cell state changes

Wright

Hall

Irish

et al. 2021

Preprint

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Alternative splicing (AS) is a key mechanism underlying cellular differentiation and a driver of complexity in mammalian neuronal tissues. However, understanding of which isoforms are differentially used or expressed and how this affects cellular differentiation remains unclear. Long read sequencing allows full-length transcript recovery and quantification, enabling transcript-level analysis of AS processes and how these change with cell state. Here, we utilise Oxford Nanopore Technologies sequencing to produce a custom annotation of a well-studied human neuroblastoma cell line and to characterise isoform expression and usage across differentiation. We identify many previously unannotated features, including a novel transcript of the voltage-gated calcium channel subunit gene, CACNA2D2. We show differential expression and usage of transcripts during differentiation, and identify a putative molecular regulator underlying this state change. Our work highlights the potential of long read sequencing to uncover previously unknown transcript diversity and mechanisms influencing alternative splicing.

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Section: Resultsmentioning

confidence: 99%

Long read sequencing reveals novel isoforms and insights into splicing regulation during cell state changes

Wright

Hall

Irish

et al. 2021

Preprint

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“…It has also been reported that eNOS expression is downregulated in PE (29). These changes result in insufficient placental VEGF and eNOS expression, and endothelial dysfunction, thereby resulting in the initiation and development of PE (26)(27)(28). Amaral et al (30) demonstrated that iNOS expression is upregulated in PE rats, and that inhibition of iNOS significantly decreases RUPP-induced increase of plasma 8-isoprostane.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have reported that VEGF plays a key role in vasculogenesis and angiogenesis, both of which are important in the development of the placenta (24,25). Increasing evidence suggests that VEGF expression is downregulated (26,27), and the placenta produces elevated levels of VEGF receptor (Flt-1), which captures free VEGF (28). It has also been reported that eNOS expression is downregulated in PE (29).…”

Section: Discussionmentioning

confidence: 99%

Effects of S1PR2 antagonist on blood pressure and angiogenesis imbalance in preeclampsia rats

et al. 2021

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Preeclampsia (PE), a hypertensive multisystem disorder, can lead to increased maternal and fetal mortality and morbidity. Sphingosine-1-phosphate (S1P) plays various roles, depending on the cell type, by binding to S1P receptors (S1PR). The present study evaluated the changes of S1PRs and investigated the potential role of S1PRs in pregnancy-induced hypertension. PE rats were established by reduced uterine perfusion pressure. The involvement of S1PR2 was evaluated using JTE-013, a specific S1PR2 antagonist, in PE rats. After the treatment, inflammatory cytokines were evaluated using enzyme linked immunosorbent assay, and the expression of vascular endothelial growth factor (VEGF), inducible nitric oxide synthase (iNOS) activation and endothelial nitric oxide synthase (eNOS) were evaluated by reverse transcription-quantitative PCR and western blotting. Results showed that S1PR2, but not S1PR1 and S1PR3, was significantly increased in the serum and placenta tissues of PE rats. Notably, JTE-013 significantly decreased blood pressure, attenuated infiltration of inflammatory cells and decreased inflammation, as indicated by the decreased expression of inflammatory cytokines, including tumor necrosis factor-α, interleukin-1β (IL-1β) and IL-6, in placental tissues. Mechanistic studies demonstrated that JTE-013 significantly increased the expression of VEGF and decreased the expression of fms-like tyrosine kinase 1 in placental tissue. Furthermore, JTE-013 prevented iNOS activation and increased eNOS in placental tissue. In summary, the present study demonstrated that S1PR2 contributed to hypertension and angiogenesis imbalance in PE rats.

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“…Despite the lack of a direct link between RBM28 and the core network, these proteins appear to play a role in a variety of cellular mechanisms, including cell cycle arrest and apoptosis induction via pre-mRNA splicing of multiple target genes, including TP53. They appear to be critical for cancer cell transformation and progression prevention in a variety of cancers, including lung cancer [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

An in silico pipeline approach uncovers a potentially intricate network involving spike SARS-CoV-2 RNA, RNA vaccines, host RNA-binding proteins (RBPs), and host miRNAs at the cellular level

Chetta

Tarsitano

Oro

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background In the last 2 years, we have been fighting against SARS-CoV-2 viral infection, which continues to claim victims all over the world. The entire scientific community has been mobilized in an attempt to stop and eradicate the infection. A well-known feature of RNA viruses is their high mutational rate, particularly in specific gene regions. The SARS-CoV-2 S protein is also affected by these changes, allowing viruses to adapt and spread more easily. The vaccines developed using mRNA coding protein S undoubtedly contributed to the “fight” against the COVID-19 pandemic even though the presence of new variants in the spike protein could result in protein conformational changes, which could affect vaccine immunogenicity and thus vaccine effectiveness. Results The study presents the findings of an in silico analysis using various bioinformatics tools finding conserved sequences inside SARS-CoV-2 S protein (encoding mRNA) same as in the vaccine RNA sequences that could be targeted by specific host RNA-binding proteins (RBPs). According to the results an interesting scenario emerges involving host RBPs competition and subtraction. The presence of viral RNA in cytoplasm could be a new tool in the virus’s armory, allowing it to improve its chances of survival by altering cell gene expression and thus interfering with host cell processes. In silico analysis was used also to evaluate the presence of similar human miRNA sequences within RBPs motifs that can modulate human RNA expression. Increased cytoplasmic availability of exogenous RNA fragments derived from RNA physiological degradation could potentially mimic the effect of host human miRNAs within the cell, causing modulation of the host cell network. Conclusions Our in silico analysis could aid in shedding light on the potential effects of exogenous RNA (i.e. viruses and vaccines), thereby improving our understanding of the cellular interactions between virus and host biomolecules. Finally, using the computational approach, it is possible to obtain a safety assessment of RNA-based vaccines as well as indications for use in specific clinical conditions.

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Role of RNA‑binding protein 5 in the diagnosis and chemotherapeutic response of lung cancer (Review)

Cited by 9 publications

References 81 publications

Long read sequencing reveals novel isoforms and insights into splicing regulation during cell state changes

Long read sequencing reveals novel isoforms and insights into splicing regulation during cell state changes

Effects of S1PR2 antagonist on blood pressure and angiogenesis imbalance in preeclampsia rats

An in silico pipeline approach uncovers a potentially intricate network involving spike SARS-CoV-2 RNA, RNA vaccines, host RNA-binding proteins (RBPs), and host miRNAs at the cellular level

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