Role of residual cytoplasm on oxidative status during sperm maturation in dogs

Angrimani, Daniel de Souza Ramos; Losano, João Diego de Agostini; Lúcio, Cristina de Fátima; Veiga, Giselle Almeida Lima da; Pereda, M. C. V.; Nichi, Marcílio; Vannucchi, Camila Infantosi

doi:10.1016/j.anireprosci.2014.10.023

Cited by 18 publications

(24 citation statements)

References 43 publications

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“…While the fertilizing capacity of sperm collected from the epididymis has been confirmed, epididymis storage temperature may be a key variable for this technique to be successful (Kaabi et al, 2003). There is a consensus that temperatures between 0 and 5°C are adequate for storing the epididymis of several species until sperm collection and processing, such as mice (An, Wada, Edashige, Sakurai, & Kasai, 1999), ram (Lone, Islam, Khan, & Sofi, 2011), red deer (Soler, Garcia, Fernándes-Santos, Esteso, & Garde, 2003), dogs (Angrimani et al, 2014;Yu & Leibo, 2002) and bulls (Nichi et al, 2007;Papa et al, 2015). Kaabi et al (2003) concluded that ram epididymal sperm samples collected from testicles stored below 5°C had higher fertilizing capacity compared with samples stored at room temperature.…”

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confidence: 99%

Evaluation of epididymis storage temperature and cryopreservation conditions for improved mitochondrial membrane potential, membrane integrity, sperm motility and in vitro fertilization in bovine epididymal sperm

Nichi

Rijsselaere

Losano

et al. 2016

Reprod Domestic Animals

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The maintaining of the epididymis at lower temperatures during storage and transport improves sperm quality. Our study aimed to test whether epididymis storage temperature (post-mortem) and sperm cryopreservation affect sperm kinetics, membrane integrity, mitochondrial potential and fertility capacity. Thirty-six epididymides were collected from 18 bulls after slaughter and divided into two groups: at 4 or 34°C for 2-3 hr. The sperm was collected from the epididymis cauda. The evaluation consisted of computer-assisted sperm analysis (CASA), SYBR14/PI/JC1 to evaluate membrane integrity, mitochondrial membrane potential (MMP) and measurement of lipid peroxidation (TBARS). The sperm was then frozen using an automatic device. After thawing, sperm samples were evaluated by the same variables and further in vitro fertilization rates. Cryopreservation negatively affected sperm motility in samples stored at 4 and 34°C. Nevertheless, the 4°C samples yielded higher rates of blastocyst formation. Pre-freeze sperm motility, progressive motility and velocity were higher in sperm from epididymis stored at 4°C while post-thaw sperm motility, progressive motility and velocity remained the same among samples from epididymis stored at 4 or 34°C. However, with regard to the kinetic patterns, samples collected from epididymis stored at 34°C had lower values when compared to those stored at 4°C prior the cryopreservation process. Our results indicate that epididymis handling conditions after cryopreservation may affect sperm quality after thawing, especially due to compromised MMP in sperm collected from epididymis stored at higher temperatures.

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mentioning

confidence: 99%

Evaluation of epididymis storage temperature and cryopreservation conditions for improved mitochondrial membrane potential, membrane integrity, sperm motility and in vitro fertilization in bovine epididymal sperm

Nichi

Rijsselaere

Losano

et al. 2016

Reprod Domestic Animals

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“…The positive correlation was shown between the percentage of motile spermatozoa and the percentage of morphologically normal spermatozoa (Agarwal et al, ; Ellington, Scarlett, Meyers‐Wallen, Mohammed, & Surman, ). The highest mitochondrial membrane potential and the maximum sperm motility occur only in the epididymal cauda in dogs (Angrimani, Losano, et al, ; Angrimani et al, ).…”

Section: Sperm Morphology Alterationsmentioning

confidence: 99%

Canine spermatozoa—What do we know about their morphology and physiology? An overview

Chłopik

Wysokińska

2019

Reprod Domestic Animals

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Spermatozoa are unique cells because of their morphological and physiological characteristics. They are produced during the process called spermatogenesis. Spermatogenesis consists of three phases: spermatocytogenesis, spermiogenesis and spermiation, during which spermatozoa undergo several changes. Spermatogenesis takes place within the seminiferous tubules containing two types of cells—the germ cells and the Sertoli cells—that alongside the Leydig cells, which play an important role when it comes to normal fertility. Everything is regulated by the hypothalamic–pituitary–gonadal axis and specific hormones due to multi‐hormonal feedback systems. Spermatozoa possess morphological and physiological features, which are sometimes completely different from what is observed in various somatic cells. What is more, canine spermatozoa have specific characteristics making them special compared to the spermatozoa of other mammalian species. The metabolic energy production, which is crucial for the appropriate functioning of spermatozoa, can be fuelled by different metabolic pathways utilizing different chemical substrates. Inseparable from the oxidative phosphorylation process is the production of reactive oxygen species, which are both essential and toxic to spermatozoa. Furthermore, epididymis is a very important structure, responsible for the transport and maturation of spermatozoa, which are then stored in the last segment of epididymis—the epididymal cauda. Moreover, the retrieval of spermatozoa from the epididymides is crucial for the development of assisted reproduction techniques and sperm cryopreservation methods. The information gained from the research on domestic dogs might be transferred to their wild relatives, especially those species categorized as endangered.

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“…Therefore, spermatozoa are extremely dependent upon the antioxidant protection provided by the reproductive tract environment (ANGRIMANI et al, 2014a). The most important antioxidant enzyme of the mammalian semen is superoxide dismutase (SOD, CASSANI et al, 2005).…”

Section: Proteins Of the Canine Spmentioning

confidence: 99%

“…The presence of GPx in the three segments of the canine epididymis might suggest that this enzyme plays an important role in the overall process of sperm maturation (ANGRIMANI et al, 2014a). GPx has an enzymatic activity in the canine SP of 3.98±0.29U ml -1 (STRZEZEK et al, 2009); it is negatively correlated with the percentage of spermatozoa with linear motility (n=20, r=-0.679, P<0.05) (NEAGU et al, 2011) and positively correlated with percentage of spermatozoa with an intact acrosome membrane (r=0.49, P=0.02) (ANGRIMANI et al, 2014a).…”

Section: Proteins Of the Canine Spmentioning

confidence: 99%