2017
DOI: 10.1016/j.freeradbiomed.2017.04.286
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Role of redox-potential decreasing in the chronic isoproterenol-induced cardiac hypertrophy and its pharmacological correction

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“…Then pellet was resuspended in 0.3 ml cold solution 2 containing: 0.4 mol/L NaCl, 20mmol/L HEPES-buffer, 1.0 mmol/L EGTA and 1.0 mmol/L EDTA, 1.0 mmol/L PMSF and 1.0 mmol/L DTT, pH 7.8, left on ice for 20 min and the pellet of centrifugated at 10,000 g for 30 min was used in the study. The activity of NF-kB(p60) was registrated as described early[29]. The Bradford method (Coomassie brilliant blue G-250 staining) was used to detect protein content.…”
mentioning
confidence: 99%
“…Then pellet was resuspended in 0.3 ml cold solution 2 containing: 0.4 mol/L NaCl, 20mmol/L HEPES-buffer, 1.0 mmol/L EGTA and 1.0 mmol/L EDTA, 1.0 mmol/L PMSF and 1.0 mmol/L DTT, pH 7.8, left on ice for 20 min and the pellet of centrifugated at 10,000 g for 30 min was used in the study. The activity of NF-kB(p60) was registrated as described early[29]. The Bradford method (Coomassie brilliant blue G-250 staining) was used to detect protein content.…”
mentioning
confidence: 99%