Role of Radical Oxygen Species in Rat Testicular Germ Cell Apoptosis Induced by Heat Stress

Ikeda, Masato; Kodama, Hiroyuki; Fukuda, Jun; Shimizu, Yasushi; Murata, Masanori; Kumagai, Jin; Tanaka, Toshinobu

doi:10.1095/biolreprod61.2.393

Cited by 166 publications

(134 citation statements)

References 44 publications

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“…Number of interstitial cells of Leydig were found to be reduced which is shown in Figure 3. Similar was reported by Ikeda M. et al [22]. As observed in the histological section, use of Vitamin E against heat stress (group IV) showed somewhat protective effect but was not much effective.…”

Section: 64±021supporting

confidence: 88%

Effect of Vitamin E Against Heat Stress Induced Testicular Damage in Wistar Albino Rats

Yadav¹,

Awasthi²,

Pandey³

et al. 2017

IJAR

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Background: Heat stress is one of the most important stressors especially in hot regions of the world. Spermatogenesis and normal testicular function are both temperature dependent. There is compelling evidence that there is a correlation between male infertility and testicular heat stress. Since oxidative stress is the major source of damage after testicular heat stress, it seems logical that antioxidant can prevent germ cell apoptosis and sperm damage. Therefore, the aim of the study was to observe the effect of vitamin E against heat stress induced testicular damage.

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Section: 64±021supporting

confidence: 88%

Effect of Vitamin E Against Heat Stress Induced Testicular Damage in Wistar Albino Rats

Yadav¹,

Awasthi²,

Pandey³

et al. 2017

IJAR

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“…It has been shown that raised temperature can lead to oxidative stress within the rat testis; this in turn can trigger apoptosis (Ikeda et al 1999). The effects of oxidative stress on the genomic integrity of spermatozoa have been studied by inducing oxidative stress in the testis and in mature spermatozoa (Aitken et al 1989, Lucesoli & Fraga 1995.…”

Section: Discussionmentioning

confidence: 99%

Impact of a mild scrotal heat stress on DNA integrity in murine spermatozoa

Banks¹,

King²,

Irvine³

et al. 2005

Reproduction

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An increase in scrotal temperature can lead to the production of poor quality spermatozoa and infertility. In the present study we have used mice to examine the impact of mild, scrotal heat stress (42 8C for 30 min) on numbers of spermatozoa as well as on the integrity of their DNA. Spermatozoa recovered from the epididymides hours (1 to 24) or days (7 to 32) after treatment were analysed using COMET and sperm chromatin structure (SCSA) assays. The treatment induced a stress response in both the testis and the epididymis that was associated with reduced expression of the cold inducible RNA binding protein (Cirp) and an increase in germ cell apoptosis (Apotag positive cells). Although spermatozoa present in the epididymis at the time of heating contained correctly packaged DNA, its integrity was compromised by heat stress. In addition, although some germ cells, which were present within the testis at the time of heat stress, were removed by apoptosis, many germ cells completed their development and were recovered as motile spermatozoa with damaged DNA. In conclusion, these data demonstrate that scrotal heat stress can compromise the DNA integrity of spermatozoa and this may have clinical implications for patients undergoing IVF and intra-cytoplasmic sperm injection (ICSI).

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“…Heat shock is also known to induce radical oxygen species, including superoxide anion (O 2 . Å ) and hydrogen peroxide (H 2 O 2 ) (Ikeda et al, 1999), and these radicals may provide an alternative pathway to ATM activation.…”

Section: Discussionmentioning

confidence: 99%

Activation of ATM and phosphorylation of p53 by heat shock

et al. 2002

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p53 protein is phosphorylated in response to various stresses. Here we examined phosphorylation of p53 protein in normal human diploid cells after heat shock at 438C for 2 h. We found that heat shock stimulates phosphorylation of p53 at Ser15 but not at Ser20, while X-irradiation at 4 Gy and 10 J/m 2 of UV induces phosphorylation of p53 at Ser15 and less signi®cantly at Ser20. Increased phosphorylation of Ser15 was also observed in heat shocked GM638, the SV40-transformed human ®broblast cell line. Although X-ray irradiation induced phosphorylation of Ser6, 9, 20, and 37 in GM638 cells, heat shock did not aect the phosphorylation level of these serines. We observed little or no phosphorylation of p53 at Ser15 in two primary ataxia telangiectasia ®broblast cells, that are defective in ATM. Using an in vitro kinase assay, we con®rmed that immunoprecipitated ATM from both heat-shocked and X-irradiated normal human diploid cells can phosphorylate p53 at Ser15 to a similar extent. These results indicate that heat shock induces phosphorylation of p53, especially at Ser15, and its phosphorylation is mediated by ATM kinase.

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Role of Radical Oxygen Species in Rat Testicular Germ Cell Apoptosis Induced by Heat Stress

Cited by 166 publications

References 44 publications

Effect of Vitamin E Against Heat Stress Induced Testicular Damage in Wistar Albino Rats

Effect of Vitamin E Against Heat Stress Induced Testicular Damage in Wistar Albino Rats

Impact of a mild scrotal heat stress on DNA integrity in murine spermatozoa

Activation of ATM and phosphorylation of p53 by heat shock

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