Role of Pyridine Nucleotides and Hexose Monophosphate Pathway in Butter Yellow (DAB) Carcinogenesis

Kotnis, L. B.; Narurkar, M. V.; Sahasrabudhe, M. B.

doi:10.1038/bjc.1962.63

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…with four values greater than the average for the control group. Our resuLlts show rather larger falls in the concentration of the nucleotides for both azo-dyes than those given in the literature (Jedeikin et al 1956;Kotnis, Narurkar & Sahasrabudhe, 1962; see Reid, 1962). Activities of NMN adenylyltran8fera8e and of NAD+ kinase.…”

Section: Precancerous Livers Produced By Azo-dyescontrasting

confidence: 46%

The concentration and biosynthesis of nicotinamide nucleotides in the livers of rats treated with carcinogens

Clark¹,

Greenbaum²,

McLean³

1966

Biochemical Journal

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1. The oxidoreduction state and concentration of both NAD and NADP as well as the maximum potential activities of NMN adenylyltransferase and NAD(+) kinase have been measured in the livers of rats treated for 14-28 days with 4-dimethylamino-3'-methylazobenzene, 4-dimethylamino-4'-fluoroazobenzene, alpha-naphthyl isothiocyanate or ethionine and in primary hepatomas induced by 4-dimethylamino-3'-methylazobenzene. 2. The total NAD and total NADP both decreased in the livers of rats treated with either azo-dyes or alpha-naphthyl isothiocyanate but not in those treated with ethionine. The activities of NMN adenylyltransferase and NAD(+) kinase did not alter appreciably after such treatments. 3. In the primary hepatomas the concentrations of both NAD and NADP fell drastically and the activities of NMN adenylyltransferase and NAD(+) kinase fell to about 50% of the control activities. 4. No correlation could be established between the concentrations of the nucleotides and the activities of the enzymes synthesizing them. It appears, however, that a relationship exists between the NAD content of the tissue and the amount of NADP present. 5. The results are discussed with respect to the control of NAD and NADP synthesis by ATP. At the concentrations of NAD normally present in the cell it is suggested that NAD may be a rate-limiting substrate in NADP synthesis.

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Section: Precancerous Livers Produced By Azo-dyescontrasting

confidence: 46%

The concentration and biosynthesis of nicotinamide nucleotides in the livers of rats treated with carcinogens

Clark¹,

Greenbaum²,

McLean³

1966

Biochemical Journal

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References

1965

Biochemical Approaches to Cancer

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Immediate Effects of Carcinogens and Related Compounds on Pyridine Nucleotides

Kotnis¹,

Narurkar²,

Sahasrabudhe³

1962

Br J Cancer

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TUMOUR growth is usually associated with low levels of pyridine nucleotides (PN)* (Glock and McLean, 1957; Jedeikin and Weinhouse, 1955). In addition, it was earlier shown from this laboratory that the levels and synthesis of pyridine nucleotides are also lowered in the host tissues of tumour bearing animals (Narurkar, Kumta and Sahasrabudhe, 1957 Narurkar and Sahasrabudhe, 1959). In all these instances, the host tissues showed a lowering of levels and synthesis of pyridine nucleotides. In view of the low levels of PN and the role PN play in energy production, a feed-back mechanism for regulation of normal growth was postulated (Sahasrabudhe, 1958). It was suggested that rapid cellular proliferation regulated its own growth through a self imposed curtailment in PN levels and therefore (though indirectly) of energy production. In malignancy, however, tumour continues to grow unrestrained in spite of the low levels of pyridine nucleotides, thus suggesting an existence of an alternative way of obtaining energy and a breakdown in the feed-back mechanism for regulation of normal growth. Since the diminished levels of PN are evident in almost every type of tumour, it was necessary to investigate whether the lowering in the pyridine nucleotides was in any way related to the factors responsible for the causation of malignant transformation. Alternatively the low PN levels may be the effect of malignant transformation, particularly since there is rapid nucleic acid synthesis. If the former possibility (i.e., the low PN levels as the causative factor of malignant transformation) be true, carcinogens should be able to produce some effects on PN levels long before malignancy sets in. This was investigated by studying the immediate or short term effects of some of the carcinogens and related compounds. MATERIALS AND METHODSWistar rats bred in our colony on stock diet were used in these experiments. The effect of the following substances was investigated: (1) p-dimethylaminoazobenzene (DAB or butter yellow), (2) 20-methylcholanthrene, (3) isoniazide, (4) semicarbazide, (5) urethane, (6) metatoluidine, (7) dimetheyl-p-phenylene-* The term " pyridine nucleotides" or " PN " denotes the sum total of DPN, DPNH TPN and TPNH.

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Role of Pyridine Nucleotides and Hexose Monophosphate Pathway in Butter Yellow (DAB) Carcinogenesis

Cited by 4 publications

References 9 publications

The concentration and biosynthesis of nicotinamide nucleotides in the livers of rats treated with carcinogens

The concentration and biosynthesis of nicotinamide nucleotides in the livers of rats treated with carcinogens

References

Immediate Effects of Carcinogens and Related Compounds on Pyridine Nucleotides

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